Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Characteristics and Frequencies of Alternative Initiation Codon(AIC) of mtDNA ND2 in Five Pig Breeds

돼지 5품종에 있어서 mtDNA ND2 유전자의 선택적 개시코돈의 특성과 빈도

  • Han, S.H. (National Institute of Subtropical Agriculture, R.D.A) ;
  • Cho, I.C (National Institute of Subtropical Agriculture, R.D.A) ;
  • Choi, Y.L. (National Institute of Subtropical Agriculture, R.D.A) ;
  • Lee, C.E. (National Institute of Subtropical Agriculture, R.D.A) ;
  • Ko, M,S. (National Institute of Subtropical Agriculture, R.D.A) ;
  • Kim, J.H. (Division of Applied Life Science, Gyeongsang National University) ;
  • Seo, B.Y. (Division of Applied Life Science, Gyeongsang National University) ;
  • Lee, J.G. (Division of Applied Life Science, Gyeongsang National University) ;
  • Jeon, J.T. (Division of Applied Life Science, Gyeongsang National University)
  • 한상현 (농촌진흥청 난지농엽연구소) ;
  • 조인철 (농촌진흥청 난지농엽연구소) ;
  • 최유림 (농촌진흥청 난지농엽연구소) ;
  • 이종언 (농촌진흥청 난지농엽연구소) ;
  • 고문석 (농촌진흥청 난지농엽연구소) ;
  • 김재환 (경상대학교 응용생명과학부) ;
  • 서보영 (경상대학교 응용생명과학부) ;
  • 이정규 (경상대학교 응용생명과학부) ;
  • 전진태 (경상대학교 응용생명과학부)
  • Published : 2004.12.31
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Abstract

Alternative initiation codon(AlC) has been reported in the mitochondrial genes in various mammalian species. We investigated the AlC of mitochondrial NADH dehydrogenase 2 gene(rntDNA ND2) in five pig breeds. Two kinds of initiation codons(ATA/ATf) showing different frequencies among tested pig breeds were used. While all Large White pigs had ATA as an initiator methionine codon, all Landrace pigs had ATf. The other breeds(Berkshire, Duroc and Hampshire) had both initiation codons with the ATA frequencies, 91.9, 21.3 and 60.00/0, respectively. In the previous reports, all Chinese indigenous pig breeds were identified to have unique initiation codon ATA. Although the effect of Ale on the translation of mtDNA ND2 has not been studied in this study, AlC patterns in mtDNA ND2 will contribute to the maternity test using molecular markers in pig breeding.

다양한 포유동물의 미토콘드리아 유전자들에서 선택적 개시코돈(AIC)들이 보고되었다. 본 연구는 돼지 5 품종에서 mtDNA ND2 유전자의 AIC 양상을 분석하였다. 두 종류의 AIC 서열들이 발견되었고, 품종 집단간에서 각기 다른 출현빈도를 보였다. ND2의 methionine codon으로 Large White와 Landrace는 각각 전 개체에서 ATA와 ATT 서열로 확인되었다. 다른 3 품종(Berkshire, Duroc, Hampshire)의 경우는 두 서열이 모두 발생하였으며, 91.9, 21.3, 60.0%의 빈도로 ATA를 보였다. 기존의 연구들에 의하면 중국재래돼지들이 모두 개시코돈 ATA를 갖는다고 보고되어 있다. 미토콘드리아 ND2 단백질의 합성과정에서 AIC가 어떤 영향을 미치는지는 설명할 수 없으나, 본 연구에서 나타난 AIC 다형성과 품종특이적인 분포양상은 돼지의 육종에 있어 모계추적을 위한 분자적 표지인자로서 이용될 수 있을 것을 사료된다.

Keywords

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