Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Immunopotentiating Activities of Cellular Components of Lactobacillus brevis FSB - 1

Lactobacillus brevis FSB - 1의 균체성분에 의한 면역증진 활성

  • Kim, Seong-Yeong (Dept. of Fodd Science and Biotechnology, Kyonggi University) ;
  • Shin, Kwang-Soon (Dept. of Fodd Science and Biotechnology, Kyonggi University) ;
  • Lee, Ho (Dept. of Fodd Science and Biotechnology, Kyonggi University)
  • 김성영 (경기대학교 식품생물공학과) ;
  • 신광순 (경기대학교 식품생물공학과) ;
  • 이호 (경기대학교 식품생물공학과)
  • Published : 2004.11.01
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Abstract

In order to evaluate the potential utilization value as a novel probiotic strain, the immunopotentiating activities of the cellular components from Lactobacillus brevis FSB-1 were examined. L. brevis FSB-1 isolated from kimchi were fractionated into the whole cell, cell wall, cytosol and extracellular preparation, and each fraction was examined on intestinal immune system modulating activity in vitro. The cell wall and cytosol preparation showed the relatively high bone marrow cell proliferating activity through Peyer's patch cell in a dose-dependent manner. But these preparations did not directly stimulate the bone marrow cell proliferation. The whole cell, cell wall and cytosol preparation also induced considerable levels of macrophage activation and mitogenicity of murine splenocytes in vitro. The anti-complementary activity (ITCH_(50)) of the cytosol fraction of L. brevis FSB-1 was the most potent in the cellular components, and the activity showed dose dependency. The complement activation by the cytosol fraction of L. brevis FSB-1 occurs via both alternative and classical pathways, which confirmed by the crossed immunoelectrophoresis using anti-human C3.

새로운 probiotic 유산균으로써의 잠재적 이용가능성을 평가할 목적으로, 김치로부터 분리한 Lactobacillus brevis FSB-1을 대상으로 각종 면역증진활성의 특성이 조사되었다. L. brevis FSB-1을 전균체, 세포벽, 세포질 및 균체외 획분으로 각각 분리하고 장관면역 활성을 측정한 결과, Peyer's patch 세포를 매개로 한 골수세포 증식활성의 경우, 세포벽 및 세포질 획분에서 상대적으로 높은 활성을 농도 의존적으로 보인 반면, 직접적인 골수세포 증식활성은 나타내지 않았다. 마크로파지의 활성화능은 전균체, 세포벽 및 세포질 획분에서 상대적으로 높은 활성을 보였으며, splenocyte mitogen 활성의 경우, 이들 획분에서 공히 대조군의 약 200%이상의 활성 증가가 관찰되었다. 그러나 양성대조군인 LPS의 활성에는 다소 미치지 못하였다. 한편 보체계 활성화능을 검토한 결과, 균체외 획분을 제외한 모든 획분에서 높은 활성을 보였으며, 특히 세포질 획분에서 농도 의존적으로 매우 강력한 활성을 나타냈다. 또한 세포질 획분에 의한 보체계 활성화는 anti-human C3를 이용한 2차원 면역전기영동에 의해 classical 및 alternative pathway 양 경로를 경유함을 확인할 수 있었다.

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