Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Galactosylated Chitosan (GC)-graft-Poly(vinyl pyrrolidone) (PVP) as Hepatocyte-Targeting DNA Carrier: In Vitro Transfection

  • Park, In-Kyu (School of Agricultural Biotechnology, Seoul National University) ;
  • Jiang, Hu-Lin (School of Agricultural Biotechnology, Seoul National University) ;
  • Cook, Seung-Eun (School of Agricultural Biotechnology, Seoul National University) ;
  • Cho, Myung-Haing (School of Agricultural Biotechnology, College of Veterinary Medicine, Seoul National University) ;
  • Kim, Su-Il (School of Agricultural Biotechnology, Seoul National University) ;
  • Jeong, Hwan-Jeong (Department of Nuclear Medicine, Wonkwang University School of Medicine) ;
  • Akaike, Toshihiro (Department of Biomolecular Engineering, Tokyo Institute of Technology) ;
  • Cho , Chong-Su (School of Agricultural Biotechnology, Seoul National University)
  • Published : 2004.01.01
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Abstract

Galactosylated chitosan-graft-poly(vinyl pyrrolidone) (GCPVP) was synthesized and characterized for hepatocyte-targeting gene carrier. GCPVP itself as well as GCPVP/DNA complex had negligible cytotoxicity regardless of the concentration of GCPVP and the charge ratio, but GCPVP/DNA complex had slightly cytotoxic effect on HepG2 cells only in the case of the higher charge ratio and 20 mM of $Ca^{2+}$ concentration used. Through the confocal laser scanning microscopy, it is shown that the endocytosis by interaction between galactose ligands of GCPVP and ASGPR of the hepatocytes was the major route of transfection of GCPVP/F-plasmid complexes.

Keywords

References

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