Archives of Pharmacal Research

The Pharmaceutical Society of Korea (대한약학회)
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Apoptosis Inducing Effects of 6-Methoxydihydrosanguinarine in HT29 Colon Carcinoma Cells

  • Lee, Yong-Jin (College of Pharmacy and MeRRI, Wonkwang University) ;
  • Yin, Hu-Quan (College of Pharmacy and MeRRI, Wonkwang University) ;
  • Kim, Young-Ho (College of Pharmacy, Chungnam National University) ;
  • Li, Guang-Yong (College of Pharmacy and MeRRI, Wonkwang University) ;
  • Lee , Byung-Hoon (College of Pharmacy and MeRRI, Wonkwang University)
  • Published : 2004.01.01
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Abstract

6-Methoxydihydrosanguinarine (6ME), a benzophenanthridine alkaloid derived from the methanol extracts of Hylomecon hylomeconoides, showed a dose-dependent effect at 1-10 ${\mu}M$ on causing apoptotic cell death in HT29 colon carcinoma cells $(IC_{50} = 5.0{\pm}0.2 {\mu}M)$. Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.

Keywords

References

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