Environmental Analysis Health and Toxicology

The Korean Society of Environmental Health and Toxicology (환경독성보건학회)
권호 표지

Evaluation of the Genetic Toxicity of Synthetic Chemicals(XIV) -In vivo Bone Marrow Micronucleus Assay of 11 Synthetic Chemicals in Mice-

합성화학물질들의 유전독성평가(XIV)-마우스의 골수세포를 이용한 11종 합성화학물질들의 생체내 소핵시험-

  • 류재천 (한국과학기술연구원, 독성연구실) ;
  • 김연정 (한국과학기술연구원, 독성연구실)
  • Published : 2004.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

합성화학물질들이 환경으로의 유입은 인체에는 물론 환경생태계에 많은 영향을 미치므로 이들의 유해성 검증은 매우 중요한 일이라 할 수 있다. 실제 산업체에서 사용되는 수많은 화학물질들의 유전적 손상 유발유무는 유해성검증에서 무엇보다 중요한 일이라 할 수 있다. 이에 산업체 공정과정에서 널리 사용되는 것으로 알려진 11종의 합성화학물질에 대해 마우스의 골수 세포를 이용한 in vivo소핵시험을 수행하여, 소핵형성 유발유무를 관찰하였다. 양성대조군으로 사용된 mitomycin C는 음성대조군과 비교시 유의하게 소핵을 유발하는 반면, 비교적 마우스에서 높은 50%치사량을 보이는 thiourea, 2,4-dichlorophenol 및 2,4-toluene diisocyanate 등의 합성물질들을 비롯한 나머지 8종의 물질들은 본 실험결과 통계적으로 유의하게 소핵을 유발하지 않는 것을 관찰 할 수 있었다.

Keywords

References

  1. Ames BN, McCann J and Yamasaki E. Method for detect-ing carcinogens and mutagens with Salmonella/mammalian-microsome mutagenicity test, Mut. Res. 1975;31: 347-364 https://doi.org/10.1016/0165-1161(75)90046-1
  2. Becker P, Scherthan H and Zankl H. Use of a centromere-specific DNA probe (P82H) in nonisotopic in situ hybridization for classification of micronuclei, Genes Chromosomes Cancer 1990; 2: 59-62 https://doi.org/10.1002/gcc.2870020111
  3. Clive D, Bolcsfoldi G, Clements J, Cole J, Homna M, Majeska J, Moore M, Muller L, Myhr B, Oberly T, Oudelhkim M-C, Rudd C, Shimada H, Sofuni T, Thy-baud V and Wilcox P. Consensus agreement regarding protocol issues discussed during the mouse lymphoma workshop, Environ. Mol. Mutagenesis 1995; 25: 165-168 https://doi.org/10.1002/em.2850250211
  4. Garriott ML, Casciano DA, Schechtman LM and Probst GS. International workshop on mouse lymphoma assay testing practices and data interpretations, Environ. Mol. Mutagenesis 1995; 25: 162-164 https://doi.org/10.1002/em.2850250210
  5. Hayashi M, Morita T, Kodama Y, Sofuni T and Ishidate M Jr. The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides, Mut. Res. 1990; 245: 245-249 https://doi.org/10.1016/0165-7992(90)90153-B
  6. Hayashi M, Kodama Y, Awog T, Suzuki T, Asita AO and Sofuni T. The micronucleus assay using peripheral blood reticulocytes from mitomycin C-and cyclophos-phamide-treated rats, Mut. Res. 1992; 278: 209-213 https://doi.org/10.1016/0165-1218(92)90236-S
  7. Hayashi M, Maki-Paakkanen J, Tanabe H, Honma M, Suzuki T, Matsuoka A, Mizusawa H and Sofuni T. Isolation of micronuclei from mouse blood and fluo-rescence in situ hybridization with a mouse centromeric DNA probe, Mut. Res. 1994; 307: 245-251 https://doi.org/10.1016/0027-5107(94)90298-4
  8. Hayashi M, Ueda T, Uyeno K, Wada K, Kinae N, Saotome K, Tanaka N, akai A, Sasaki YF, Asano N, Sofuni T and Ojima Y. Development of genotoxicity assay systems that use aquatic organisms, Mut. Res. 1998; 399: 125-133 https://doi.org/10.1016/S0027-5107(97)00251-0
  9. Heddle JA. A rapid in vivo test for chromosomal damage, Mut.Res. 1973; 18: 187-190 https://doi.org/10.1016/0027-5107(73)90035-3
  10. Heo M-Y, Kim J-H and Ryu J-C. Anticlastogenicity of beta-carotene and galangin using in vivo supravital staining micronucleus test, Environ. Mutagens & Car-cinogens 1997; 17: 92-96
  11. IARC (International Agency for Research on Cancer). IARC monographs on the evaluation of carcinogenic risks to humans, Supplement 7, Overall Evaluation of Carcino-genicity: An updating of IARC monographs from Vols. 1 to 42, IARC, Lyon, 1987
  12. Ishidate M and Odashima S. Chromosome test with 134 compounds on chinese hamster cells in vitro-A screen-ing for chemical carcinogens. Mut. Res. 1977; 48: 337-354 https://doi.org/10.1016/0027-5107(77)90177-4
  13. Komae N, Hibino Y and Sugano N. Analysis of micronuclei induced under hyperthermic conditions in human lym-phocyte culture by fluorescence in situ hybridization(FISH) and spectral karyotyping (SKY) methods, Yakugaku Zasshi 1999; 119: 763-772
  14. Lorke D. A new approach to practical acute toxicity testing, Arch. Toxicol. 1983; 54: 275-287 https://doi.org/10.1007/BF01234480
  15. Maron DM and Ames BN. Revised methods for the Salmonella mutagenicity test, Mut. Res. 1983; 113: 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  16. MacGregor JT, Wehr CM, Henika PR and Shelby MD. The in vivo erythrocyte micronucleus test: Measurement at steady state increases assay efficiency and permits integration with toxicity studies, Fundam. Appl. Toxicol. 1990, 14(3): 513-522 https://doi.org/10.1016/0272-0590(90)90255-I
  17. McCann J, Choi E, Yamasaki E and Ames BN. Detection of carcinogens as mutagens in the Salmonella/microsome test : Assay of 300 chemicals, Proc. Natl. Acad. Sci. USA 1975; 72: 5135-5139 https://doi.org/10.1073/pnas.72.12.5135
  18. Meselson M and Russell K. Comparison of carcinogenesis and mutagenesis potency. Origin of Human Cancer edited by Hiatt HH, Watson JD and Winstend JA. Cold Spring Harbor Laboratory, New York, 1991; pp. 1473-1481
  19. Miller BM, Zitzelsberger HF, Weier H-Ul G and Adler I-D. Classification of micronuclei in murine erythrocytes: Immunofluorescent staining using CREST antibodies compared to in situ hybridization with biotinylated gamma satellite DNA, Mutagenesis 1991; 6: 297-302 https://doi.org/10.1093/mutage/6.4.297
  20. Morita T, Asano N, Awogi T, Sasaki YF, Sato S, Shimada H, Sutou S, Suzuki T, Wakata A, Sofuni T and Hayashi M. Evaluation of the rodent micronucleus assay in the screening of IARC carcinogens (groups 1, 2A and 2B): The summary report of the 6th collaborative study by CSGMT/JEMS.MMS, Mut. Res. 1997; 389: 3-122 https://doi.org/10.1016/S1383-5718(96)00070-8
  21. Peace BE and Succop P. Spontaneous micronucleus frequency and age: What are normal values? Mut. Res. 1999; 425: 225-230 https://doi.org/10.1016/S0027-5107(99)00030-5
  22. Ryu J-C, Lee S, Kim K-R and Park J. Evaluation of the genetic toxicity of synthetic chemicals (I). Chromo-somal aberration test on chinese hamster lung cells in vitro, Environ. Mutagens & Carcinogens 1994; 14: 138-144
  23. Ryu J-C, Kim K-R, Ryu E-K, Kim H-J, Kwon O-S, Song C-E, Mar W and Chang I-M. Chromosomal aberration assay of taxol and 10-deacetyl baccatin III in chinese hamster lung cells in vitro, Environ. Mutagens & Carcinogens 1996a; 16: 6-12
  24. Ryu J-C, Kim K-R, Kim H-J, Ryu E-K, Lee S-Y, Jung S-O, Youn J-Y, Kim M-H and Kwon O-S. Evaluat-ion of the genetic toxicity of synthetic chemicals (II). A pyrethroid insecticide, fenpropathrin, Arch. Pharm. Res. 1996b; 19:251-257
  25. Ryu J-C, Kim K-R, Kim H-J, Myung S-W, Kim G-H, Lee M-J and Chang I-M. Genotoxicity Study of Bojungchisup-tang, an oriental herbal decoction-in vitro chromosome aberration assay in chinese hamster lung cells and in vitro supravital-staining micronucleus assay with mouse peripheral reticulocytes, Arch. Pharm. Res. 1998; 21(4): 391-397
  26. Ryu J-C, Kim K-R and Choi Y-J. in vitro mouse lymp-homa thymidine kinase (tk+/-) gene forward mutation assay in mammalian cells, Environ. Mutagens & Carci-nogens 1999; 19(1): 7-13
  27. Ryu J-C, Kim K-R, Lee S and Park J. Evaluation of the genetic toxicity of synthetic chemicals (III), Chro-mosomal aberration assay with 28 chemicals in chinese hamster lung cells in vitro, Environ, Mutagens & Carcinogens 2001a; 21(1): 14-22
  28. Ryu J-C and Park KY. Anticlastogenic effect of Baechu(Chinese cabbage) Kimchi and Buchu (leek) Kimchi in supravital staining micronucleus assay using peripheral reticulocytes of mouse, Environ. Mutagens & Car-cinogens 2001b; 21(1): 51-56
  29. Ryu J-C, Kim KR and Kim YJ. Evaluation of the Genetic Toxicity of Synthetic Chemicals (IV)-in vitro chromo-somal aberration assay with 18 chemicals in Chinese hamster lung cells-, Environ. Mutagens & Carcinogens 2002a; 22(3): 149-156
  30. Ryu J-C, Kim KR, Kim YJ and Choi HY. Evaluation of the Genetic Toxicity of Synthetic Chemicals (V)-in vitro chromosomal aberration assay with 17 chemicals in Chinese hamster lung cells-, Environ. Mutagens & Carcinogens 2002b; 22(4): 215-222
  31. Ryu J-C, Kim KR, Kim YJ and Jeon HK. Evaluation of the genetic toxicity of synthetic chemicals (VI)-in vitro chromosomal aberration assay with 17 chemicals in Chinese hamster lung cells-, J. Environ. Toxicol. 2003a; 18(2): 111-120
  32. Ryu J-C and Kim KR. Evaluation of the genetic toxicity of synthetic chemicals (VII)-a synthetic selective herbi-cide, Pendimethalin-, J. Environ. Toxicol. 2003b; 18(2): 121-129
  33. Ryu J-C, Kim KR and Kim YJ. Evaluation of the genetic toxicity of synthetic chemicals (VIII)-in vivo bone marrow micronucleus assay of 8 synthetic chemicals in mice, J. Environ. Toxicol. 2003c; 18(2): 137-143
  34. Ryu J-C and Kim YJ. Evaluation of the genetic toxicity of synthetic chemicals (IX)-a synthetic selective herbicide, Pretilachlor-, J. Environ. Toxicol. 2004; 19(1): 93-100
  35. Saotome K, Sofuni T and Hayashi M. A micronucleus assay in sea urchin embryos, Mut. Res. 1999; 446: 121-127 https://doi.org/10.1016/S1383-5718(99)00155-2
  36. Schmid W. The micronucleus test, Mut. Res. 1975; 31: 9-15 https://doi.org/10.1016/0165-1161(75)90058-8
  37. Tanisho T, Yagi T, Iwasaki K, Shimoi K, Kinae N, Hayashi M and Sofuni T. Monitoring of coastal water contam-inated with mutagens and/or carcinogens using micronu-cleus test in fish, Environ. Mutagen Res. 1998; 20: 1 -9
  38. Tice RR, Erexson GL, Hilliard CJ, Huston JL, Boehm RM, Gulati D and Shelby MD. Effect of treatment protocol and sample time on the frequencies of micronucleated polychromatic erythrocytes in mouse bone marrow and peripheral blood, Mutagenesis 1990; 5(4): 313-321 https://doi.org/10.1093/mutage/5.4.313
  39. Yamamoto KI and Kikuchi Y. A comparison of diameters of micronuclei induced by clastogens and by spindle poisons, Mut. Res. 1980; 71: 127-131 https://doi.org/10.1016/0027-5107(80)90012-3