Environmental Analysis Health and Toxicology

환경독성보건학회 (The Korean Society of Environmental Health and Toxicology)
권호 표지

디젤분진의 수용성 추출물에 의한 메탈로치오닌 유전자 발현

Expression of Metallothionein mRNA in Diesel Exhaust Particles Treated A549 Cell

  • 발행 : 2004.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Metallothionein gene expression of diesel exhaust particles (DEP) was investigated in human lung epithelial cell line. DEP was collected from diesel motor bus and soluble fraction in water was obtained. Cells, grown to near confluence, were exposed to 5-50 ppm DEP for 6 hours. Regarding the metallothionein gene expressions, MT-1 and MT-2 were induced in the DEP-treated cell by using RT-PCR and real-time PCR. However, MT-3 which is known to be brain specific, and another isoform MT-4 were not expressed in cadmium-treated groups as well as control group. Heavy metal of DEP was also analyzed and Zn was found as the major component of heavy metals in DEP used in this study.

키워드

참고문헌

  1. 박광식, 구자민. 인간폐포세포 유래 A549세포주에서의Cadmium 처리에 의한 메탈로치오닌 유전자 발현, 환경독성학회지 2003; 18(1): 15-19
  2. Amakawa K, Terashima T, Matsuzaki T, Matsumaru A,Sagai M and Yamaguchi K. Suppressive effects of diesel exhaust particles on cytokine release from human and murine alveolar macrophages. Exp. Lung Res. 2003; 29(3): 149-64 https://doi.org/10.1080/01902140303770
  3. Bunger J, Muller MM, Krahl J, Baum K, Weigel A, Hallier E and Schulz TG. Mutagenecity of diesel exhaust particles from two fossil and two plant oil fuels. Mutagenesis 2000; 15(5): 391-397 https://doi.org/10.1093/mutage/15.5.391
  4. Carero A, Hoet PH, Cerschaee L, Schoeters G and Nemery B. Genotoxic effects of carbon black particles, diesel exhaust particles , and urban air particulates and their extracts on a human alveolar epithelial cell line (A549) and a human monocytic cell line (THP-1). Environ Mol. Mutagen, 2001; 37(2): 155-63 https://doi.org/10.1002/em.1023
  5. Coyle P, Philcox JC, Carey LC and Rofe AM. Metalloth-ionein: The multipurpose protein. Cellular and Mole-cular Life Science 2002; 59: 627-647 https://doi.org/10.1007/s00018-002-8454-2
  6. Hiura TS, Li N, Kaplan R, Horwitz M, Seagrave JC and Nel AE. The role of a mitochondrial pathway in the induc-tion of apoptosis by chemicals extracted from diesel exhaust particles. J. Immunol. 2000; 165(5): 2703-2711 https://doi.org/10.4049/jimmunol.165.5.2703
  7. Hiura TS, Kaszubowski MP, Li N and Nel AE. Chemicals in diesel exhaust particles generate reactive oxygen radi-cals and induce apoptosis in macrophages. J. Immunol. 1999; 163(10): 5582-5591
  8. Ishihara Y and Kagawa J. Dose-response assessment and effect of particles in guinea pigs exposed chronically to diesel exhaust; analysis of various biological markers in pulmonary alveolar lavage fluid and circulating blood. Inhal. Toxicol. 2002; 14(10): 1049-1067 https://doi.org/10.1080/08958370290084773
  9. Jiang SS, Chen TC, Yang JY, Hsiung CA, Su IJ, Liu YL, Chen PC and Juang JL. Sensitive and quantitative detec-tion of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction. Clin. Infect. Dis. 2004; 38(2): 293-296 https://doi.org/10.1086/380841
  10. Kagawa J. Health effects of diesel exhaust emissions; a mix-ture of air pollutants of worldwide concern. Toxicology 2003; 181-182, 349-353
  11. Kodavanti UP, Moyer CF, Ledbetter AD, Schladweiler MC, Costa DL, Hauser R, Christiani DC and Nyska A. Inhal-ed environmental combustion particles cause myocardial injury in the Wistar Kyoto rat. Toxicol. Sci. 2003; 71(2):237-245 https://doi.org/10.1093/toxsci/71.2.237
  12. Lambert E, Kille P and Swaminathan R. Cloning and sequ-encing a novel metallothionein I isoform expressed in human reticulocytes. FEBS Letters 1996; 389: 210-212 https://doi.org/10.1016/0014-5793(96)00587-X
  13. Lee C, Jeon SH, Na JG and Park K. Sequence analysis of choriogenin H gene of medaka (Oryzias latipes) and mRNA expression. Environ. Toxicol. Chem. 2002; 21 (8): 1709-1714 https://doi.org/10.1897/1551-5028(2002)021<1709:SAOCHG>2.0.CO;2
  14. Madden MC, Dailey LA, Stonehuerner JG and Harris BD Responses of cultured human airway epithelial cells treated with diesel exhaust extracts will vary with the engine load. J. Toxicol. Environ. Health A 2003; 66(24): 2282-2297
  15. Marano F, Boland S, Bonvallot V, Baulig A and Baeza-Squiban A. Human airway epithelial cells in culture for studying the molecular mechanisms of the inflammatory response triggered by diesel exhaust particles. Cell Biol. Toxicol. 2002; 18(5): 315-320 https://doi.org/10.1023/A:1019548517877
  16. Nemmar A, Hoet PH, Dinsdale D, Vermylen J, Hoylaerts MF and Memery B. Diesel exhaust particles in lung acutely enhance experimental peripheral thrombosis. Circulation. 2003; 107(8): 1202-1208 https://doi.org/10.1161/01.CIR.0000053568.13058.67
  17. Palmiter RD, Findley SD, Whitmore TE and Dumam DM MT-III, a brainspecific member of the metallothionein gene family. Proc. Natl. Acad. Sci. USA 1992; 89: 6333-6337 https://doi.org/10.1073/pnas.89.14.6333
  18. Pfaffl MW, Gerstmayer B, Bosio A and Windisch W. Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats; monitoring gene expres-sion using cDNA microarrays combined with real-time RT-PCR. J. Nutr. Biochem. 2003; 14(12): 691-702 https://doi.org/10.1016/j.jnutbio.2003.08.007
  19. Pohjola SK, Lappi M, Honkanen M and Savela K, Compari-son of mutagenicity and calf thymus DNA adducts formed by the paniculate and semivolatile fractions of vehicle exhausts. Environ. Mol. Mutagen. 2003; 42(1): 26-36 https://doi.org/10.1002/em.10172
  20. Salvi S, Blomberg A, Rudell B, Kelly F, Sandstrom T, Holgate ST and Frew A. Acute inflammatory responses in the airways and peripheral blood after short-term exposure to diesel exhaust in healthy human volunteers. Am. J. Respir. Crit. Care Med, 1999; 153(3): 702-709
  21. Scheepers PT and Bos RP. Combustion of diesel fuel from a toxicological perspective II. Toxicity. Int. Arch. Occup. Environ. Health. 1992; 64(3): 163-177 https://doi.org/10.1007/BF00380905
  22. Schmidt CJ, Jubier MF and Harmer DH. Structure and expression of two human metallothionein-I isoform genes and a related pseudogene. J. Biol. Chem. 1985; 260(12): 7731-7737
  23. Wang HW, Chen FW, and Ueng TH. Induction of cytochro-mes P-450 1A1 and 1B1 by motorcycle exhaust parti-culate in human breast cancer MCF-7 cells. J. Toxicol. Environ. Health A 2002; 65(19): 1401-1417 https://doi.org/10.1080/00984100290071478
  24. Watanabe N and Oonuki Y, Inhalation of diesel engine exhaust affects spermatogenesis in growing male rats. Environ. Health Perspect. 1999; 107(7): 539-544 https://doi.org/10.2307/3434395