Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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$H_2$ $O_2$ Resistance of Escherichia coli That Expresses Acetyl Xylan Esterase of Streptomyces coelicolor A3(2)

Streptomyces coelicolor A3(2)의 Acetyl Xylan Esterase를 발현하는 Escherichia coli의 과산화수소 저항성

  • 김재헌 (단국대학교 미생물학과) ;
  • 최원일 (단국대학교 미생물학과) ;
  • 윤석원 (단국대학교 의학레이저연구센터) ;
  • 정상운 (단국대학교 의학레이저연구센터, 국립환경연구원 환경위해성연구부 위해성평가과) ;
  • 오충훈 (단국대학교 의학레이저연구센터)
  • Published : 2004.09.01
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Abstract

We investigated hydrogen peroxide resistance of Escherichia coli possessing acetyl xylan esterase(AxeA) of Streptomyces coelicolor A3(2). The induction of AxeA production by isopropyl-$\beta$-thiogalactoside was confirmed by SDS-polyacrylamide gel electrophoresis. The differences in growth between induced and non-induced E. coli were determined by the changes in optical density of cultures after hydrogen peroxide treatment The lethal effect of hydrogen peroxide was observed for non-induced cultures at all concentrations tested in this study (lmM, 2.5mM and 5mM). However, cultures induced for AxeA production resisted the lethal effect, except at 5mM where cells were killed irrespective of the AxeA production. The axeA induction increased survival against 1.5mM hydrogen peroxide from 59% to 74%. In addition, AxeA producing E. coli showed increased survival at $45^{\circ}C$, near maximum growth temperature. Therefore, it was concluded that AxeA conferred a cross-resistance upon the bacterium against both oxidative- and heat stress.

Streptomyces coelicolor A3(2)의 acetyl xylan esterase (AxeA)가 Escherichia coli의 과산화수소 저항성에 미치는 영향을 알아보고자 하였다. AxeA 발현은 isopropyl-$\beta$-thiogalactoside로 유도되었고 생산된 AxeA는 SDS-polyacrylamide gel electrophoresis방법으로 확인하였다. AxeA 발현에 따른 과산화수소 저항성의 변화를 E. coli의 생장곡선과 생존율을 통하여 조사하였다. AxeA가 발현되지 않으면 모든 처리 농도 (1 mM, 2.5mM, 5mM)에서 균의 사멸이 일어났다. AxeA가 발현되는 조건에서는 5mM을 제외한 과산화수소 1mM와 2.5mM에서 E. coli의 사멸이 저지되었다. 또한 1.5mM의 과산화수소에 대한생존율이 59%에서 74%로 높다졌다. 동시에 E. coli의 최고생장온도에서에 근접한 $45^{\circ}C$에서의 생존율도 증가되는 결과를 얻었다. 그러므로 AxeA 단백질은 산화적 스트레스와 온도스트레스에 대해 교차 저항성을 나타내는 역할을 한다고 결론지었다.

Keywords

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