A Molecular Phylogenetic Study on Korean Alexandrium catenella and A. tamarense Isolates (Dinophyceae) Based on the Partial LSD rDNA Sequence Data

  • 발행 : 2004.09.01

초록

Sequences of the large subunit ribosomal (LSD) rDNA D1-D2 region of Alexandrium catenella(=A. sp. cf. catenella) and A. tamarense isolates, which were collected along the Korea coasts, were analyzed to understand their phylogenetic relationships and geographical distributions. All A. catenella and A. tamarense isolates belonged to the A. tamarense/catenella/fundyense complex and were grouped with the North American and temperate Asian ribotypes, respectively, regardless of the presence or absence of a ventral pore in the first apical plate. A consistent and peculiar characteristic that differentiated the Alexandrium isolates was amplification of a second PCR product with a lower molecular weight in addition to the predicted one; ten A. catenella isolates belonging to the temperate Asian ribotype yielded this additional PCR product. Sequence alignment revealed that the shorter PCR product resulted from an unusual large deletion of 87 bp in the LSD rDNA D1 domain. The North American and temperate Asian ribotypes were prevalent along the Korean coasts without geographical separation. Given the high genetic homogeneity among widely distributed Alexandrium populations, each ribotype appeared to be pandemic rather than to constitute a distinct regional population.

키워드

참고문헌

  1. Adachi, M., Y. Sako, Y., Ishida, D.M. Anderson and B. Reguera, 1993. Cross-reactivity of five monoclonal antibodies to vari- ous isolates of Atexandrium as determined by an indirectimmunofluorescence method. Nippon Suisan Gakkaishi, 59: 1807
  2. Balech, E., 1995. The Genus Alexandrium Halim (Dinflagellata). Sherkin Island Marine Station, Co. Cork, 151 pp
  3. Cavalier-Smith, T, 1993. Kingdom Protozoa and its 18 phyla. MicrobioI. Rev. 57: 953-994
  4. Cembella, A.D. and F.J.R. Taylor, 1986. Electrophoretic variabil-ity within the Protogonyautax tamarensis/cateneIta species complex: pyridine linked dehydrogenases. Biochem. Syst. Ecot., 14:311-323
  5. Cembella, A.D., J.J. Sulli'van, G.L. Boyer, F.J.R. Taylor and R.J.Andersen, 1987. Variation in paralytic shellfish toxin compo- sition within the Protoeonyaulax tamarensis/catenetIa spe- cies complex: red tide dinoflagellates. Biochem. Syst. EcoL, 15:171-186 https://doi.org/10.1016/0305-1978(87)90018-4
  6. Cembella, A.D., F.J.R. Taylor and J.C. Therriault, 1988. Cladistic analysis of electrophoretic variants within the toxic dinoflagellate genus Protogonyaulax.Bot. Mar., 31: 39-51
  7. Chang, D.S., I.S. Shin, J.H. Pyeun and Y.H. Park, 1987. A study on paralytic shellfish poison of sea mussel, MytiIus edutis,Korea. Butt. Kor. Fish. Soc. 20: 293-299 (in Korean)
  8. Cho, C.H., 1978. On the Gonyautax red tide in Jinhae Bay. Bull. Kor. Fish. Soc., 11: 111-114 (in Korean)
  9. Darling, K.R, C.M. Wade, I.A. Stewart, D. Kroon, R. Dingle andA.J.L. Brown, 2000. Molecular evidence for genetic mixingof Arctic and Antarctic subpolar populations of planktonic foraminifers. Nature, 405: 43-47 https://doi.org/10.1038/35011002
  10. Daugbjerg, N., G. Hansen, J. Larsen and $\phi$. Moestrup, 2000. Phy-logeny of some of the major genera of dinoflagellates based on ultrastructure and partial LSU rDNA sequence data,including the erection of three new genera of unarmored dinoflaeellates. Phycotogia, 39: 302-317
  11. de Vargas, C., R. Norris, L. Zaninetd, S.W. Gibb and J. Pawlowski, 1999. Molecular evidence of cryptic speciation in planktonic fora-minifers and their relation to oceanic province. Proc. Natt. Acad. Sci. USA, 96: 2864-2868
  12. Delgado, M., E. Garces, M. Vila and J. CamP, 1997. Morphologi-cal variability in three populations of the dinoflagellate Atex andrium taylori. J. Ptankton Res.. 19: 749-757
  13. Felsenstein, J., 1985. Confidence limits on phylogenies: an approachusing the bootstrap. Evotution, 38: 16-24
  14. Fukuyo, Y., 1985. Morphology of Protogonyautax tamarensis(Lebour) Taylor and Protogonyaulax catenella (Whedon andKofoid) Taylor from Japanese coastal waters. Butt. Mar. Sci., 37:529-537
  15. Gunderson, J.H., M.L. Sogin, G. Wollett, M. Hollingdale, V.F. de la Cruz and T.F. McCutchan, 1987. Structurally distinct, stage-specific ribosomes occur in PIasmodium. Science, 238: 933-937
  16. Hallegraeff, G.M., 1993. A review of harmful algal blooms andtheir apparent global increase. Phycotogia, 32: 79-99
  17. Hansen, G., N. Daugbjerg and J.M. Franco, 2003. Morphology, toxin composition and LSU rDNA phytogeny of Atexandriumminutum (Dinophyceae) from Denmark, with some morpho-logical observations on other European strains. HarmfutAtgae, 2: 317-335
  18. Hayhome, B.A., D.M. Anderson, D.M. Kulis and D.J. Whitten, 1989. Variation among congeneric dinoflagellates from the northeastern United States and Canada. I. Enzyme electro-phoresis. Mar. BioL, 101: 427-435
  19. Higman, W.A., D.M. Stone and J.M. Lewis, 2001. Sequence com-parisons of toxic an non-toxic Atexandrium tamarense (Dino phyceae) isolates from UK waters. Phycotogia, 40: 256-262
  20. Hong, Y.-K., S.-D. Kim, M. Polne-Fuller and A. Gibor, 1995.DNA extraction conditions from Porphyra perforata usingLiCl. J. Appt. PhycoI., 7: 101-107
  21. Kim, K.-Y., M. Yoshida, Y. Fukuyo and C.-H. Kim, 2002. Mor-phological observation of AIexandrium tamarense (Lebour)Balech, A. catenetta (Whedon and Kofoid) Balech and relatedone morphotype (Dinophyceae) in Korea. Atgae, 17: 11-19
  22. Lee, J.S., I.S. Shin, Y.M. Kim and D.S. Chang, 1997. Paralyticshellfish toxins in the mussel, MytiIus edutis, caused theshellfish poisoning accident at Geoje, Korea, in 1996. J. Kor.Fish. Soc., 30: 158-160 (in Korean)
  23. Lee, S.Y., H.W. Hee, J.G. Park, J.A. Lee and Y.S. Park, 1998. Molecular phylogeny of phytoplankton isolated from red tides in southern coast of Korea. Bada. 3: 90-93 (in Korean)
  24. Medlin, L.K., M. Lange, U. Wellbrock, G. Donner, M. Elbr$\"{a}$hter, C. Hummert and B. Luckas, 1998. Sequence comparisons link toxic European isolates of Atexandrium tamarense from the Orkney isolates to toxic North American stocks. Eur. J. Protistol, 34: 329-335
  25. Mylvaganam, S. and P.P. Dennis, 1992. Sequence heterogeneity between the two genes encoding 16S rRNA from the halophilic archaebacterium Haloarcula marismortui. Genetics, 130: 399-410
  26. N$\"{u}$bel, U., B. Engelen, A. Felske, J. Snaider, A. Wieshuber, R.I. Amann, W. Ludwig and H. Backhaus, 1996. Sequence heterogeneities of genes encoding 16S rRNAs in Paenibacillus polymyxa detected by temperature gradient gel electrophoresis. J. Bacteriol, 178: 5636-5643
  27. Olsen, G.J., C.R. Woese and R. Overberbeek, 1994. The winds of (evolutionary) change: Breathing new life into microbiology. J. Bacteriol., 176: 1-6
  28. Posada, D. and K.A. Crandall, 1998. MODELTEST: testing the model of DNA substitution. Bioinformatics, 14: 817-818
  29. Rehnstam-Holm, A.-S., A. Godhe and D.M. Anderson, 2002. Molecular studies of Dinophysis (Dinophyceae) species from Sweden and North America. Phycologia, 41: 348-357
  30. Saldarriaga, J.F., F.J.R. Taylor, P.J. Keeling and T. Cavalier-Smith, 2001. Dinoflagellate nuclear SSU rRNA phylogeny suggests multiple plastid losses and replacements. J. Mot. Evol., 53: 204-213
  31. Saunders, G.W., D.R.A. Hill, J.P. Sexton and R.A. Andersen, 1997. Small-subunit ribosomal RNA sequences from selected dinoflagellates: testing classical evolutionary hypothesis with molecular systematic methods, In: Origins of Algae and their Plastids, edited by Bhattacharya, D., Springer, New York, pp. 237-259
  32. Scholin, C.A. and D.M. Anderson, 1996. LSU rDNA-based RFLP assays for discriminating species and strains of Alexandrium (Dinophyceae). J. Phycol., 32: 1022-1035
  33. Scholin, C.A., D.M. Anderson and M.L. Sogin, 1993. The existence of two distinct small-subunit rRNA genes in the North American toxic dinoflagellate Alexandrium fundyense (Dinophyceae). J. Phycol., 29: 209-216
  34. Scholin, C.A., M. Herzog, M. Sogin and D.M. Anderson, 1994. Identification of group- and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). II. Sequence analysis of a fragment of the LSU rRNA gene. J. Phycol., 30: 999-1011
  35. Scholin, C.A., G.M. Hallegraeff and D.M. Anderson, 1995. Molecular evolution of the Alexandrium tamarense 'species complex' (Dinophyceae): dispersal in the North American and West Pacific regions. Phycotogia, 34: 472-485
  36. Swofford, D.L., 2001. 'PAU$P^{*}$: Phylogenetic Analysis Using Parsimony (* and Other Methods), Version 4.0b8', Sinauer, Sunderland
  37. Thompson, J.D., D.G. Higgins and T.J. Gibson, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting position-specific gap penalties and weight matrix choice. Nucteic Acids Res., 22: 4673-4680
  38. Uribe, P.C., B.A. Su$\'{a}$rez-Isla and R.T. Espejo, 1999. Ribosomal RNA heterogeneity and identification of toxic dinoflagellate cultures by heteroduplex mobility assay. J. Phycol., 35: 884-888
  39. van Oppen, M.J.H., J.L. Olsen, W.T. Stam, C. van den Hoek and C. Wiencke, 1993. Arctic-antarctic disjunctions in the benthic seaweeds Acrosiphonia arcta (Chlorophyta) and Desmarestia viridis/willii (Phaeophyta) are of recent origin. Mar. Biol., 115: 381-386
  40. Yentsch, C.M., B. Dale and J.W. Hurst, 1978. Coexistence of toxic and non-toxic dinoflagellates resembling Gonyaulax tamarensis in New England coastal waters. J. Phycol., 14: 330-332
  41. Yeung, P.K.K., K.F. Kong, F.T.W. Wong and J.T.Y. Wong, 1996. Sequence data for two large-subunit rRNA genes from an Asian strain of AIexandrium catenella. Appl. Environ. Microbiol., 62: 4199-4201
  42. Zardoya, R., E. Costas, V. L$\'{o}$pez-Rodas, A. Garriod-Pertierra and J.M. Bautista, 1995. Revised dinoflagellate phytogeny inferred from molecular analysis of large-subunit ribosomal RNA gene sequences. J. Mol. Evol., 41: 637-645