Ocean and Polar Research

  • 제26권4호
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  • Pages.551-557
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  • 2004
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  • 1598-141X(pISSN)
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  • 2234-7313(eISSN)
한국해양과학기술원 (Korea Institute of Ocean Science & Technology)
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참돔의 lipoprotein lipase 유전자 다형성

Polymorphisms of the Lipoprotein Lipase Gene of Red Seabream, Pagrus major

  • 장요순 (한국해양연구원 해양생물자원연구본부) ;
  • 홍경표 (한국해양연구원 해양생물자원연구본부) ;
  • 노충환 (한국해양연구원 해양생물자원연구본부)
  • 발행 : 2004.12.31
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초록

Polymorphism of the lipoprotein lipase (LPL) gene which plays an important role in regulation of lipid deposition was analysed in two red seabream (pagrus major) populations (KF4, cultured KORDI line, n=100 : JPN, imported from Japan, n=100). We amplified a DNA fragment (1,091 bp) including the exon 2 region of the LPL gene, and conducted PCR-RFLP analysis using MspI and AluI. The PCR products were also sequenced. Two alleles (A and B) were found in MspI digestion and Sve alleles (A, B, C, D and E) in AluI digestion. The sequenced data revealed four nucleotide substitutions including one transversion at the MspI recognition site (nt 2,235, $C{\rightarrow}10$) and three transitions at the AluI recognition sites (nt 1,721, $A{\rightarrow}G;$ nt 2,319, $C{\rightarrow}T;$ nt 2,319, $T{\rightarrow}C$). Among them, substitutions at the nt 2,235 and 2,319 sites which are located in the exon 2 were proved to be silent point mutations. MspI polymorphism resulted in 3 genotypes, and the allele frequency was significantly different between the two fish populations, KF4 and JPN. In the case of AluI polymorphism, the 5 alleles (A, B, C, D, E) comprised 12 genotypes of the 5 alleles. KF4 population, alleles D and I were specific to the LPL gene Polymorphisms would be useful DNA markers for red seabream population.

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참고문헌

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