Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High Frequency Shoot Formation and Plant Regeneration from Cotyledonary Hypocotyl Explants of Boxthorn (Lycium chinense Mill.) Seedlings

구기자나무(Lycium chinense Mill.) 유식물체의 자엽하배축으로부터 고효율의 부정아 형성과 식물체 재생

  • 조만현 (충청남도농업기술원 원예연구과) ;
  • 함인기 (충청남도농업기술원 원예연구과) ;
  • 이봉춘 (충청남도농업기술원 청양구기자시험장) ;
  • 김재훈 ((주)마이크로프랜츠) ;
  • 이원석 ((주)마이크로프랜츠) ;
  • 권석윤 (한국생명공학연구원 환경생명공학연구실) ;
  • 이행순 (한국생명공학연구원 식물세포공학연구실) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구실)
  • Published : 2004.09.01
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Abstract

To establish high frequency shoot formation from two cultivars (Cheongyangjaerae and Myungan) of boxthorn (Lycium chinense Mill.), hypocotyl segments with cotyledons from seedlings were used as explants. High frequency adventitious shoot formation (more than 80%) were obtained from hypocotyl segments with cotyledon on MS medium supplemented with 1.0 mg/L zeatin, when precultured for 3 weeks under dark conditions followed by transfer to light conditions. But there was no shoot induction in the explants cultured without preculture under dark conditions. Roots were induced from the shoots when transferred to rooting medium supplemented with 1.0 mg/L IAA for 4 weeks. Regenerated plantlets were grown to normal mature plants in soil.

두 품종 (청양재래, 명안)의 구기자나무 (Lycium chinense Mill.) 유식물체를 자엽하배축의 자엽 일부분만 남기고 절단 후, 절단면이 1.0 mg/L zeatin이 첨가된 MS배지에 접하도록 하여 암소에서 3주 배양한 후 명소로 옮겨 배양하였을 때 자엽하배축 절단면으로부터 부정아가 고빈도 (80% 이상)로 유도되었다. 그러나 자엽하배축절편을 암소에서 전 배양하지 않고, 명소에서만 배양 하였을 경우 부정아는 유도되지 않았다. 자엽하배축 절단면에서 유도된 부정아를 분리하여 1.0 mg/L IAA가 함유된 발근배지에서 4주간 배양하여 뿌리를 유도하였다. 잎과 뿌리가 잘 발달한 소식물체는 화분에 옮겨 키웠을 때 정상적인 형태의 재분화 식물체로 성장하였다.

Keywords

References

  1. Choi YE, Yang DC, Yoon ES, Choi KT (1999) Plant regeneration via direct embryo axis-like shoot and root formation from excised cotyledon explants of ginseng seedlings. In Vitro Cell Dev Biol 35: 210-213 https://doi.org/10.1007/s11627-999-0079-9
  2. Choi YE, Yang DC, Yoon ES, Choi KT (1998) Plant regeneration via adventitious bud formation from cotyledon explants of Panax ginseng C. A. Meyer. Plant Cell Rep 17: 731-736 https://doi.org/10.1007/s002990050474
  3. Choi PS, Cho DY, Soh WY (2003) Plant regeneration from immature embryo cultures of Vigna unguiculata. Biol Plantarum 47: 305-308
  4. Colojin-Hooymans CM, Hakkert JC, Jansen J, Custers JBM (1994) Competence for regeneration of cucumber cotyledons is restricted to specific development stages. Plant Cell Tiss Org Cult 39: 211-217 https://doi.org/10.1007/BF00035972
  5. Dong JZ, Jia SR (1991) High efficiency plant regeneration from cotyledons of watermelon (Citrullus vulgaris Schrad.). Plant Cell Rep 9: 559-562
  6. Fujiwara K, Kozai T (1995) Physical microenvironment and its effects. In: J Aitken-Christie, T Kozai, ML Smith, Automation and environmental control in plant tissue culture, Kluwer Academic Publishers, Dordrecht, pp 319-369
  7. Gambley RL, Dodd WA (1990) An in vitro technique for the production de novo of multiple shoots in cotyledon explants of cucumber (Cucumis sativus L.). Plant Cell Tiss Org Cult 20: 177-183
  8. Ham IK, Kim HS, Lee MA, Jo MH, Lee EM (1999) Growth characteristics of tissue cultured plantlets by lighting direction and light intensity. Korean J Plant Res 12: 139-144
  9. Hachey JE, Sharma KK, Moloney MM (1991) Efficient shoot regeneration of Brassica campestris using cotyledon explants cultured in vitro. Plant Cell Rep 9: 549-554
  10. Hotta M, Ogata K, Nitta A, Hosikawa K, Yanagi M, Yamazaki K (1989) Sekai yuhyou-shokubutu jiten (useful plant of the world). Heibonsha, Tokyo, pp 640-641
  11. Jeong BR, Fujiwara K, Kozai T (1995) Environmental control and photoautotrophic micropropagation. Hort Rev 17: 125-172
  12. Jo MH, Ham IK, Lee MA, Lee EM, Song NH, Han GH, Woo IS (2002a) Effects of sealing materials and photosynthetic photon flux of culture vessel on growth and vitrification in carnation plantlets in vitro. J Korean Soc Hort Sci 43: 133-136
  13. Jo MH, Ham IK, Park SK, Lee BC, Han GH, Woo IS (2002b) Plant regeneration through leaf explant culture of boxthorn. Korean J Hort Sci Technol 20 (suppl. Ⅱ): 134
  14. Kim BW, Choi MS, Roh KS, Park YG (1993) Somatic embryogenesis from leaf callus of Lycium chinense Mill. Kor J Plant Tissue Cult 20: 91-96
  15. Kim DC, Chung HJ, Min BH, Yang DC (2002) Plant regeneration from explant types and cultivars of boxthorn (Lycium chinense Mill). Korean J Plant Biotechnology 29: 15-18 https://doi.org/10.5010/JPB.2002.29.1.015
  16. Kim JW, Han SK, Kwon SY, Lee HS, Lim YP, Liu JR, Kwak SS (2000) High frequency shoot induction and plant regeneration from cotyledonary hypocotyl explants of cucumber (Cucumis sativus L.) seedlings. J Plant Physiol 157: 136-139 https://doi.org/10.1016/S0176-1617(00)80147-9
  17. Lee BC, Kim SD, Yun TS, Lee BH, Ra SW, Park YC, Woo IS, Kim DH, Jeon TS (2000a) Production of new cultivar in boxthorn (Lycium chinense Mill.). Research reports of Chungcheongnam-do Agriculture Research and Extension Services, Yesan, Korea, pp 417-424
  18. Lee EM, Jo MH, Song NH, Woo IS, Lee YB, Kwak SS (2000b) Light influences, morphogenesis and protein content on callus differentiation of cucumber (Cucumis sativus L.). Korean J Plant Tiss Cult 27: 213-217
  19. Lee JS, Kwon KW, Bae CH, Yang DC (2001) Advanced regeneration and genetic transformation of Lycium chinense harboring salt tolerance genes. Korean J Plant Tiss Cult 28: 47-52
  20. Li W, Zhang DW (1990) Medical and aromatic perennial crops. In: Ammirato PV, Evans DA, Sharp WR, Yamada Y, Handbook of plant cell culture, Vol 6, McGraw-Hill, USA, pp 116-126
  21. Liu CS (1991) An efficient method for selecting embryogenic callus from Lycium chinense Mill. cell culture. Plant Sci 79: 99-103 https://doi.org/10.1016/0168-9452(91)90075-J
  22. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  23. Park YG, Choi MS, Kim BW, Chung WI, Noh KS (1995) Factors affecting introduction of rolC gene in Lycium chinense Mill. Korean J Plant Tiss Cult 22: 329-334
  24. Yakov IR, Vladimr AR, Nickolai MP (1990) Regeneration of Lycium barbarum L. plant from leaf tissue, callus culture and callus protoplasts. Plant Cell Rep 9: 84-87