Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of E. coli Phosphofructokinase Gene in an Autotrophic Bacterium Acidithiobacillus thiooxidans

  • Tian, Ke-Li (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Lin, Jian-Qun (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Liu, Xiang-Mei (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Liu, Ying (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Zhang, Chang-Kai (State Key Laboratory of Microbial Technology, Shandong University) ;
  • Yan, Wang-Ming (State Key Laboratory of Microbial Technology, Shandong University)
  • Published : 2004.02.01
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Abstract

A plasmid pSDK-l containing the Escherichia coli phosphofructokinase-l gene (pfkA) was constructed, and transferred into extremely acidophilic Acidithiobacillus thiooxidans Tt-7 by conjugation with the aid of plasmid RP4 at a frequency of $10^{-5}$ per recipient. This plasmid was stable in A. thiooxidans. The pfkA gene from E. coli could be expressed in this obligately autotrophic bacterium, but the enzyme activity (21.6 U/g protein) was lower than that in E. coli (K12: 85.9 Dig protein; DF1010 carrying plasmid pSDK-l: 96.6 U/g protein). In the presence of glucose, the Tt-7 transconjugants consumed glucose, leading to a better growth yield.

Keywords

References

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