한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제33권8호
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  • Pages.1268-1272
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  • 2004
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  • 1226-3311(pISSN)
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  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
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병원성 식중독 미생물에 대한 천년초 선인장 추출물의 항균 활성

Antimicrobial Effect of the Extracts of Cactus Chounnyouncho(Opuntia humifusa) against Food Borne Pathogens

  • 이경석 (호서대학교 식품생물공학과) ;
  • 김민규 (호서대학교 식품생물공학과) ;
  • 이기영 (호서대학교 식품생물공학과)
  • Lee, Kyung-Seok (Dept. of Food and Biotechnology, Hoseo University) ;
  • Kim, Min-Gue (Dept. of Food and Biotechnology, Hoseo University) ;
  • Lee, Ki-Young (Dept. of Food and Biotechnology, Hoseo University)
  • 발행 : 2004.10.01
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초록

본 연구에서는 천년초 손바닥 선인장 유기용매 추출물의 항균성을 비교, 검토하고자 하였다. 70% ethanol로 추출하여 hexan, chloroform, ethyl acetate, buthanol, water로 순차 분획하여 ascorbic acid, benzoic acid와 항균효과를 비교하여 보았다. Escherichia coli 등 Gram positive bacteria 2종, Gram negative bacteria 2종, yeast 1종 등 총 6개의 병원성 식중독 미생물에 대해 항균효과를 검토한 결과 ethyl acetate 분획물이 가장 우수한 항균효과를 나타내었고 이는 동량의 benzoic acid보다 월등히 우수하였다. Gram positive bacteria와 Gram negative bacteria에 대해 유의적 차이가 없는 비슷한 항균효과를 보여주었으나 bacteria에 비해 yeast에서 항균효과는 낮게 나타났다.

The 70% ethanol extract from cactus Chounnyouncho (Opuntia humifusa) was fractionated subsequently by hexan, chloroform, ethyl acetate, buthanol and water. The ethyl acetate fraction exhibited strong antimicrobial activities by paper disk diffusion method on the five strains of food born bacteria Bacillus subtilis, Staphylococcus aureus subsp. aureus, Escherichia coli, Salmonella typhimurium and Pseudomonas fluorescens. It has a heat resistance to show inhibitory effect after heat treatment at 11$0^{\circ}C$ for 1 hr. The ethyl acetate fraction showed almost perfect growth inhibition at over 700 ppm on the tested strains.

키워드

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