Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Isolation and Expression of Aluminum Induced Protein(ClAIP) Gene from Codonopsis lanceolata

더덕에서 Aluminum Induced Protein (ClAIP) 유전자의 분리 및 발현분석

  • Published : 2004.10.01
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Abstract

A cDNA clone (GenBank accession no. CF924621) homologous to aluminum induced protein gene was isolated and characterized from Codonopsis lanceolata (ClAIP). The ClAIP is 906 nucleotides long and has an open reading frame of 711 bp with a deduced amino acid sequence of 236 residues. The ClAIP shows high homology to A. marina (84%), G. hirsutum(83%), V. radiata (83%), A. thaliana (80%), B. nap us (78%) and T. aestivum (68%). The deduced amino acid sequence of ClAIP also has homology to the N-terminal end of plant Asn synthetase. This region does not contain the active sites of the enzyme and the significance of this conservation is currently not clear. To investigate the expression of ClAIP against several heavy metal stresses, we treated the sliced tap root of C. lanceolata with various heavy metals. The expression of ClAIP was increased by 25 uM $Al_2$(SO$_3$)$_4$ in proportion to incubation time and also increased by 50 uM CdCl$_2$.

더덕의 뿌리로부터 aluminum스트레스와 관련이 있는 aluminum induce protein(ClAIP)유전자를 분리하였다. ClAIP 유전자의 염기서열를 분석한 결과 906 bp 길이로, 236개의 아미노산으로 번역되는 711bp의 ORF를 가지고 있으며, A. marina(84%), G. hirsutum(84%), V. radiata(83%), A. thaliana(80%), B. hapus(78%), T. aestivum(68%) 등 다른 식물에서 밝혀져 있는 aluminum induce protein과 높은 상동성을 나타내었고, N-terminal에는 Asn synthetase영역이 존재하고 있다. 더덕에서 분리한 aluminum induced protein(ClAIP)을 aluminum처리 농도와 시간에 따른 ClAIP유전자의 발현양상을 알아보고자 50uM $Al^{3+}$ 를 처리 후 시간대별로 RT-PCR을 수행한 결과 시간이 지남에 따라 ClAIP유전자의 발현이 증가되는 것으로 나타났다 중금속, 염 , 온도에 대한 유전자의 발현양상을 조사하기 위 해 50uM CdCl,$_2$, 20 uM CuSO$_4$, 50uM Fe$_2$O$_3$, 100 uM NaCl를 2일과 42$^{\circ}C$에서 4시간 처리 후 발현량을 조사한 결과 카드뮴(Cd)에 대해서 특이적으로 반응하는 것으로 나타났다.

Keywords

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