Immobilized ${\beta}-Cyclodextrin$ as a Simple and Recyclable Method for Cholesterol Removal in Milk

  • Kwak, H.-S. (Department of Food Science and Technology, Sejong University) ;
  • Kim, S.-H. (Department of Food Science and Technology, Sejong University) ;
  • Kim, J.-H. (Department of Applied Chemistry, Sejong University) ;
  • Choi, H.-J. (Department of Food Science and Technology, Sejong University) ;
  • Kang, J. (Department of Applied Chemistry, Sejong University)
  • 발행 : 2004.08.01

초록

This study was designed to determine the optimum conditions of three different factors (mixing time, mixing temperature, and tube size) in reduction of cholesterol in milk using immobilized $\beta$-CD beads. Immobilized $\beta$-CD glass beads were prepared at different conditions of silaniza-tion and $\beta$-CD immobilization reactions. In result, the glass beads (diameter 1 mm) at 20 mM 3-isocyanatopropyltriethoxysilane and 30 mM $\beta$-CD without base showed the highest choles-terol removal rate as 41%. Using above immobilized $\beta$-CD glass beads, the cholesterol removal rate was 40.2% with 6 h of mixing time in 7 mm diameter tube at $10^{\circ}C$. After choles-terol removal from milk, the glass beads were washed for cholesterol dissociation and reused. In recycling study, the cholesterol removal rate was 41%, which was mostly same as that using new glass beads. These results indicated that cholesterol removal rate was about 40% with $\beta$-CD immobilized glass beads, however, the recycling efficiency was almost 100%.

키워드

참고문헌

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