Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Enhanced Production of Succinic Acid by Metabolically Engineered Escherichia coli with Amplified Activities of Malic Enzyme and Fumarase

  • Hong, Soon-Ho (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering and Bioprocess Engineering Research Center) ;
  • Lee, Sang-Yup (Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical & Biomolecular Engineering and Bioprocess Engineering Research Center, Department of Biosystems and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology)
  • Published : 2004.07.01
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Abstract

A pfl ldhA double mutant Escherichia coli strain NZN 111 was used to produce succinic acid by overexpressing the E. coli malic enzyme gene (sfcA). This strain, however, produced a large amount of malic acid as well as succinic acid. After the analyses of the metabolic pathways, the fumB gene encoding the anaerobic fumarase of E. coli was co-amplified to solve the problem of malic acid accumulation. A plasmid, pTrcMLFu, was constructed, which contains an artificial operon (sfcA-fumB) under the control of the inducible trc promoter. From the batch culture of recombinant E. coli NZN 111 harboring pTrcMLFu, 7 g/L of succinic acid was produced from 20 g/L of glucose, with no accumulation of malic acid. From the metabolic flux analysis the strain was found under reducing power limiting conditions by severe reorientation of metabolic fluxes.

Keywords

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