Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Fabrication of Protein A-Viologen Hetero Langmuir- Blodgett Film for Fluorescence Immunoassay

  • Lee, Woochang (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Chun, Bum-Suk (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Oh, Byung-Keun (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Lee, Won-Hong (Department of Chemical and Biomolecular Engineering, Sogang University) ;
  • Park, Jeong-Woo (Department of Chemical and Biomolecular Engineering, Sogang University)
  • Published : 2004.07.01
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Abstract

Protein A molecular thin film was fabricated as a platform of antibody-based biosensor. For the immobilization of the protein A thin film, a viologen multilayer was built up using the Langmuir-Blodgett (LB) technique, and then, protein A was adsorbed on the viologen LB film by an electrostatic interaction force, which was formed as a hetero-film structure. For the deposition of viologen, surface pressure area ($\pi$-A) isotherm was investigated. The fabricated protein A-viologen hetero LB film was investigated using atomic force microscopy (AFM). Using the developed molecular film, antibody immobilization and fluorescence measurement was carried out.

Keywords

References

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