Journal of Microbiology and Biotechnology

  • 제14권4호
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  • Pages.783-788
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  • 2004
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Production of Recombinant Proteins as Immuno-Analytical Markers of Genetically-Modified Organisms (GMO)

  • Hwang, Ok-Hwa (Graduate School of Biotechnology, Korea University) ;
  • Park, Hyuk-Gu (Graduate School of Biotechnology, Korea University) ;
  • Paek, Eui-Hwan (BioDigit Laboratories Corp., Technology Incubation Center, Biotechnology Building, Korea University) ;
  • Paek, Se-Hwan (Graduate School of Biotechnology, Korea University, Department of Biotechnology, Korea University) ;
  • Park, Won-Mok (Graduate School of Biotechnology, Korea University)
  • 발행 : 2004.08.01
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초록

Marker proteins of genetically-modified organisms (GMO) and their antibodies were prepared and characterized as major components of an analytical system. We selected two GMO markers, neomycin phosphotransferase II and 5- enolpyruvylshikimate-3-phosphate synthase, and produced them from E. coli employing genetic recombination technology. After purification, their structural conformation and binding affinities to the respective antibodies were characterized. The results showed that the recombinant proteins were identical with commercially obtained reference proteins. We further used them as immunogens to raise polyclonal antibodies capable of discriminating GMO containing protein from non-GMO. Well-characterized marker proteins and antibodies will be valuable as immunoreagents in constructing analytical systems such as biosensors and biochips to measure quantities of GMO.

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