Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Molecular Detection and Characterization of Orf Virus from Outbreak of Contagious Pustular Dermatitis in Korean Indigenous Goats

한국 재래 산양의 전염성 농피성 피부병에서 orf virus의 검출과 B2L 유전자를 통한 계통발생학적 분석

  • Park, Jin-Ho (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Kim, Guk-Jong (Kim's Animal Clinic) ;
  • Choi, Wook (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Kim, Eun-Ha (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Han, Jae-Chul (Livestock Development and Research Institute) ;
  • Ou, Sung-Guk (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Lee, Jon-Hwa (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Cho, Mae-Rim (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Song, Hee-Jong (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University) ;
  • Chae, Jun-Seok (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
  • 박진호 (전북대학교 수의과대학, 생체안전성 연구소) ;
  • 김국중 (김동물병원) ;
  • 최욱 (전북대학교 수의과대학, 생체안전성 연구소) ;
  • 김은하 (전북대학교 수의과대학, 생체안전성 연구소) ;
  • 한재철 (전라북도 축산진흥연구소 익산지소) ;
  • 어성국 (전북대학교 수의과대학, 생체안전성 연구소) ;
  • 이존화 (전북대학교 수의과대학, 생체안전성 연구소) ;
  • 조매림 (전북대학교 수의과대학, 생체안전성 연구소) ;
  • 송희종 (전북대학교 수의과대학, 생체안전성 연구소) ;
  • 채준석 (전북대학교 수의과대학, 생체안전성 연구소)
  • Published : 2004.06.01
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Abstract

Orf virus (ORFV), a member of genus Parapoxvirus (family-Poxviridae), a causative agent of contagious ecthyma in sheep and goat leading to a condition commonly known as vesicular dermatitis. Recently, twelve goats from Iksan in Jeonbuk province were observed with clinical signs like necrotic vesicular lesions around the mucosa of mouth, nasal cavity, eye, ear, teats, abdomen and groin. Based on these clinical symptoms, contagious ecthyma infection was suspected. The skin scrapping was collected from lesions for isolation of DNA and subsequent PCR amplification of ORFV specific 235 bp region of B2L gene. All of the samples were found positive by PCR analysis. Sequencing and further phylogenetic analysis of the PCR product revealed 100% identity to Japan isolate of ORFV (Okinawa, GenBank accession number AB080769), and showed 99.6% of similarity to New Zealand strain (NZ-2, GenBank accession number U06671). It was concluded that ORFV strain detected in the present study is homologous to Japan isolate and New Zealand strain. The PCR test based on amplification of B2L gene is a highly useful tools for rapid and specific diagnosis of contagious ecthyma.

Keywords

References

  1. Buttner M, Ecthyma of sheep and goats. Tierarztl Prax 1985; 13: 163-169
  2. Crumbie A. The orf virus: a disease of the fantling community. Community Nurse 1998; 4: 44-45
  3. Dela Concha-Bermejillo, Guo A, Zhang J, Waldron D. Severe persistent orf in young goats. J Vet Diagn Invest 2003; 15: 423-431 https://doi.org/10.1177/104063870301500504
  4. Dieterich RA, Spencer GR, Burger D, Gallina AM, VanderSchalie J, Contagious ecthyma in Alaskan musk-oxen and Dall sheep. J Am Vet Med Assoc 1981; 179: 1140-1143
  5. East NE, Birnie EF. Diseases of the udder, Vet Clin North Am Large Anim Pract 1983; 5: 591-600
  6. Fenner F. Poxviruses. In: Fields, B.K., Knipe, D.M., Howley, P.M. (Eds), Fields Voirology, 3rd ed. Philadelphia PA: Lippincott-Raven. 1996: 2673-2702
  7. Freeman G, Bron AJ, Juel-Jensen B. Ocular infection with orf virus. Am J Ophthalmol 1984; 97: 601-604
  8. Guss SB. Dairy goat herd health problems. J Am Vet Med Assoc 1975; 167: 1076-1079
  9. Haig DM, Mercer AA. Ovine diseases. Orf. Vet Res 1998; 29: 311-326
  10. Housawi F, Abu M, Elzein EM, al Afaleq AI, Amin MM. Sero-surveillance for orf antibodies in sheep and goats in Saudi Arabia employing the ELISA technique. J Comp Pathol 1992; 106: 153-158 https://doi.org/10.1016/0021-9975(92)90044-U
  11. Inoshima Y, Morooka A, Sentsui H. Detection and diagnosis of parapoxvirus by the polymerase chain reaction. J Virol Methods 2000; 84: 201-208 https://doi.org/10.1016/S0166-0934(99)00144-5
  12. Inoshima Y, Murakami K, Wu D, Sentsui H. Characterization of parapoxviruses circulating among wild Japanese serows (Capricornis crispus). Microbiol Immunol 2002; 46: 583-587
  13. Inoshima Y, Yamamoto Y, Takahashi T, Shino M, Katsumi A, Shimizu S, Sentsui H. Serological survey of parapoxvirus infection in wild ruminants in Japan in 1996-9. Epidemiol Infect 2001; 126: 153-156
  14. Jun MH, Cho SW, Chang KS, Cho YS, An DJ, An SW. Studies on isolation of Contagious Pustular Dermatitis (Orf) Virus from native black goats and its immunogenicity. J Kor Soc Virology 1995; 25: 189-198
  15. Linnabary RD, Powell HS, Holscher MA, Walker BK. Contagious ecthyma (Orf) in a goat herd. Vet Med Small Anim Clin 1976; 71: 1261-1263
  16. Mangana VO, Markoulatos P, Koptopoulos G, Nomikou K, Bakandritsos N, Papadopoulos O. Sheep poxvirus identification by PCR in cell cultures. J Virol Methods 1999; 77: 75-79 https://doi.org/10.1016/S0166-0934(98)00138-4
  17. Mercer AA, Yirrell DL, Whelan EM, Nettleton PF, Pow I, Gilray JA, Reid HW, Robinson AJ. A novel strategy for detennining protective antigens of the parapoxvirus, orf virus. Virology 1997; 229: 193-200 https://doi.org/10.1006/viro.1996.8433
  18. Mikarni T, Onuma M, Yonemichi H, Okada K, Fujimoto Y. Isolation of paravaccinia virus from sheep. Nippon Juigaku Zasshi 1978; 40: 109-113 https://doi.org/10.1292/jvms1939.40.109
  19. Nettleton PF, Brebner J, Pow I, Gilray JA, Bell GD, Reid HW. Tissue culture-propagated orf virus vaccine protects lambs from orfvirus challenge. Vet Rec 1996; 138: 184-186 https://doi.org/10.1136/vr.138.8.184
  20. Reid HW. Orf, In Martin, W. B., and Aitken, I. D. (eds), Diseases of sheep, 3rd ed. Oxford: Blackwell Science. 2000: 261-266
  21. Robinson AJ, Balassu TC. Contagious pustular dennatitis (orl). Vet Bulletin 1981; 51: 771-782
  22. Robinson RA. Sheep and goat zoonoses. Vet Clin North Am Large Anim Pract 1983; 5: 711-717
  23. Samuel WM, Chalmers GA, Stelfox JG, Loewen A, Thomsen JJ. Contagious ecthyma in bighorn sheep and mountain goat in western Canada. J Wildlife Dis 1975; 11: 26-31
  24. Sullivan JT, Mercer AA, Fleming SB, Robinson AJ. Identification and characterization of an orf virus homologue of the vaccinia virus gene encoding the major envelope antigen p37K. Virology 1994; 202: 968-973 https://doi.org/10.1006/viro.1994.1420
  25. Suzuki T, Minamoto N, Sugiyama M, Kinjo T, Suzuki Y, Sugimura M, Atoji Y. Isolation and antibody prevalence of a parapoxvirus in wild Japanese serows (Capricorniscrispus). J Wildlife Dis 1993; 29: 384-389
  26. Timoney JF, Gillespie JH, Scott FW, Barlough JE. Contagious pustular dennatitis (orl), In hagan's and Brunner's Microbiology and Infectious disease of domestic animals, 8th ed. Ithaca: Comstock Publishing Associates. 1998: 587-590
  27. Torfason EG, Gunadottir S. Polymerase chain reaction for laboratory diagnosis of orf virus infections. J Clin Virol 2002; 24: 79-84 https://doi.org/10.1016/S1386-6532(01)00232-3
  28. Ueda N. Detection of orf virus cross-reacting antigens with other poxvirus antigens using monoclonal antibodies. J Wakayama Med Soc 1996; 47: 443-451