Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
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Formation of an Intestine-Cartilage Composite Graft for Tracheal Reconstruction

기관 재건을 위한 장과 연골의 복합 이식판 개발

  • Jheon, Sang-Hoon (Dept. of Thoracic and Cardiovascular Surgery, College of Medicine, Catholic University of Daegu) ;
  • Lee, Sub (Dept. of Thoracic and Cardiovascular Surgery, College of Medicine, Catholic University of Daegu) ;
  • Jung, Jin-Yong (Dept. of Anesthesiology, College of Medicine, Catholic University of Daegu) ;
  • Kong, Jun-Hyuk (Biomedical Research Institute, Kyungpook National University) ;
  • Lim, Jeong-Ok (Biomedical Research Institute, Kyungpook National University) ;
  • Kim, Yu-Mi (Biomedical Research Institute, Kyungpook National University) ;
  • Jin, Chun-Jin (Biomedical Research Institute, Kyungpook National University) ;
  • Park, Tae-In (Biomedical Research Institute, Kyungpook National University) ;
  • Lee, jae-Ik (Dept. of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine) ;
  • Sung, Seok-Whan (Dept. of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine) ;
  • Choh, Joong-Haeng (Dept. of Thoracic and Cardiovascular Surgery, Seoul National University College of Medicine)
  • 전상훈 (대구가톨릭대학교 의과대학 흉부외과학교실) ;
  • 이섭 (대구가톨릭대학교 의과대학 흉부외과학교실) ;
  • 정진용 (대구가톨릭대학교 의과대학 마취과학교실) ;
  • 공준혁 (경북대학교 생명과학연구소) ;
  • 임정옥 (경북대학교 생명과학연구소) ;
  • 김유미 (경북대학교 생명과학연구소) ;
  • 김광춘 (경북대학교 생명과학연구소) ;
  • 박태인 (경북대학교 생명과학연구소) ;
  • 이재익 (서울대학교 의과대학 흉부외과학교실) ;
  • 성숙환 (서울대학교 의과대학 흉부외과학교실) ;
  • 조중행 (서울대학교 의과대학 흉부외과학교실)
  • Published : 2004.06.01
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Abstract

Background: Tracheal transplantation is necessary in patients with extensive tracheal stenosis, congenital lesions and other oncologic conditions but bears. many critical problems compared to other organ transplantations. The purpose of this study was to develop intestine-cartilage composite grafts for potential application in tracheal reconstruction by free intestinal graft. Material and Method: Hyaline cartilage was harvested from trachea of 2 weeks old New Zealand White Rabbits. Chondrocytes were isolated and cultured for 8 weeks. Cultured chondrocytes were seeded in the PLGA scaffolds and mixed in pluronic gel Chondrocyte bearing scaffolds and gel mixture were embedded in submucosal area of stomach and colon of 3 kg weighted New Zealand White Rabbits under general anesthesia. 10 weeks after implantation, bowels were harvested for evaluation. Result: We identified implantation site by gross examination and palpation. Developed cartilage made a good frame for shape memory. Microscopic examinations included special stain s howed absorption of scaffold and cartilage formation even though it was not fully matured. Conclusion: Intestine-cartilage composite graft could be applicable in the future as tracheal substitute and should be further investigated.

배경: 암이나 협착 등의 각종 기관질환으로 광범위한 기관절제가 필요한 경우에는 기관 이식이 필요하나, 다른 장기의 이식술과 비교하여 많은 어려움이 있다. 이에 본 연구에서는 이상적인 기관 대체물을 개발하기 위한 노력의 일환으로, 조직 공학적 기법을 통하여 기관 재건에 적용할 수 있는 소장-연골 복합 이식판의 개발이 가능한가를 알아보고자 하였다. 대상 및 방법: 생후 2주 된 토끼의 기관과 이개로부터 각각 연골세포를 채취하여 8주간 배양하였다. 배양된 초자 연골세포와 탄성 연골세포를 담체(PLGA)에 심거나 플루로닉 겔에 혼합한 후에, 4 종류의 혼합체를 토끼의 위장과 대장의 점막하 조직에 이식하고 10주 후에 연골 형성 여부를 평가하였다. 결과: 육안과 촉진으로 이식 부위를 판별할 수 있었으며, 현미경적 소견상 담체의 흡수와 연골의 형성을 확인할 수 있었다. 특히 초자 연골세포-담체 혼합체에서 연골의 형태를 잘 갖추고 있었다. 결론: 장-연골 복합 이식판 개발의 전망은 밝으며, 이상적인 기관 대체물로서 기관 재건에 기여할 가능성이 있다고 사료된다.

Keywords

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