Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Effect of Antioxidant on Development of Embryos in Korean Native Goats

한국 재래산양의 체외수정란 생산에 대한 항산화제의 효과

  • C. Y. Choi (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A.) ;
  • D. S. Son (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • Y. K. Kim (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • M. H. Han (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • U. G. Kweon (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • S. H. Choi (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • Y. H. Choy (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • S. B. Choi (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • Y. M. Cho (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A.)
  • Published : 2004.04.01
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Abstract

본 연구는 우리나라 고유의 유전자원인 재래산양의 체내수정란 생산기술을 확립하고자 수행하였다. 흡입법(aspiration)과 세절법(slicing)에 의해 난소 한 개당 회수된 난자의 수는 3.9개와 4.1개를 나타내어 slicing방법이 aspiration방법보다는 많은 숫자의 난자를 회수하였으나 유의적인 차이는 나타내지 않았다. 회수된 난자의 등급별 분포는 aspiration방법에서 Grade I, Grade II, Grade III, Grade IV의 비율이 10.3%, 20.5%, 38.5%, 30.8%를 나타내었으며, slicing법에서는 9.8%, 22.0%, 39.0%, 29.3%를 나타내어 Grade III과 Grade IV의 비율이 70% 이상을 차지하였다. 회수된 난자를 체외성숙 시킨 결과 Grade I과 Grade II에서는 85% 이상이 metaphase H (MII)까지 도달하였으나, Grade III과 Grade IV는 40% 이하의 체외성숙율을 나타내었다. 체외수정용 배양액으로 BO를 사용하였을 경우 Grade I 및 II에서 84.4%의 난분할율을 나타내어 TALP를 사용하였을 때의 58.8%보다 높은 난할율을 보였다. 또한 배양액의 종류별 체외발달율에 있어서는 상실배 및 배반포기배로의 발달은 mSOF를 배양액으로 이용하였을 경우 15.0%의 발달율을 나타내었으며, 체외배양 시 항산화물질인 glutathione (GSH)을 첨가함으로서 26.8%의 상실배 및 배반포배로의 발달율을 나타내었다.

Keywords

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