Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Development of Superovulation Method in Korean Native Goats

한국 재래산양의 과배란 처치 방법 개선

  • C. Y. Choi (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A.) ;
  • D. S. Son (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • Y. K. Kim (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • M. H. Han (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • U. G. Kweon (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • S. H. Choi (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • Y. H. Choy (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • S. B. Choi (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A) ;
  • Y. M. Cho (Animal Genetic Resources Station, National Livestock Research Institute, R. D. A.)
  • Published : 2004.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

한국 재래산양 체내수정란 생산에 대한 발정동기화 및 과배란 유도방법과 회수된 수정란의 동결 융해 후 생존율을 조사하였다. 발정동기화를 위해 CIDR+FSH 및 CIDR+PMSG의 방법을 이용한 결과, 배란점 및 회수된 수정란의 수는 CIDR+FSH 처리구에서 16.3개 및 9.4개, CIDR+PMSG 처리구에서 16.4개 및 8.7개를 나타내어 두 처리구간에 유의적 차이는 인정되지 않았다. 회수된 수정란을 형태학적으로 평가한 결과 CIDR+FSH 처리구에서 Gade A, B, C 및 D는 75.8%, 15.2%, 4.5% 및 4.5%를 나타낸 반면 CIDR+PMSG 처리구에서는 52.5%, 16.4%, 16.4% 및 14.8%였으며, 이식 가능한 수정란 (Grade A, B) 수는 CIDR+FSH 처리구가 유의적(P<0.05)으로 높게 나타났다. 회수된 수정란의 완만 동결 융해 후 생존성은 CIDR+FSH 처리구에서 73.3%, CIDR+PMSG 처리구에서 63.3%이었으며, 두 군간의 유의적차이는 인정되지 않았다. 따라서 본 결과는 한국 재래산양 체내수정란의 생산과 회수된 수정란의 보존을 위해서 CIDR+FSH로 발정동기화 시키는 것이 효과적이었다.

Keywords

References

  1. Baril G, Casamitjana P, Perrin J and Vallet JC. 1989. Embryo production, freezing and transfer in Angora, Alpine, and Saanen goats. Zughthyg., 24:101-115
  2. Baril G, Traldi Al, Cognie Y, Leboeuf B, Beckers JF and Mermillod P. 2001. Successful direct transfer of vitrified sheep embryos. Theriogenology, 56:299-305 https://doi.org/10.1016/S0093-691X(01)00564-7
  3. Bilton RJ and Moore NW. 1976. In vitro culture, storage and transfer of goat embryos. Austr. J. Biol. Sci., 29:125-129
  4. Chemineau P, Procureur R, Cognie Y, Lefevre PC, Locatelli A and Chupin D. 1986. Production, freezing and transfer of embryos from a bluetongue-infected goat herd without bluetongue transmission. Theriogenology, 26:279-290 https://doi.org/10.1016/0093-691X(86)90147-0
  5. Choe CY, Rho GJ and Choe SY. 1998. Effect of gonadotropin treatments on ovarian response, ovulation and embryo production in rabbits. Kor. J. Emb. Trans., 13:127-137
  6. Colleau JJ, Heyman Y and Renard JP. 1998. Les biotechnologies de la reproduction chez les bovins et leurs applications reelles ou potentielles en selection. INRA. Prod. Anim., 11:41-56
  7. Dobrinsky JR. 2002. Advancements in cryopreservation of domestic animal embryos. Theriogenology, 57:285-302 https://doi.org/10.1016/S0093-691X(01)00672-0
  8. Edwards LJ, Batt PA, Gandolfi F and Gardner DK. 1997. Modifications made to culture medium by bovine oviduct epithelial cells: Changes to carbohydrates stimulate bovine embryo development. Mol. Reprod. Dev., 46:146-154 https://doi.org/10.1002/(SICI)1098-2795(199702)46:2<146::AID-MRD5>3.0.CO;2-Q
  9. Gary ML and Raymond WWJr. 1983. Bovine embryo morphology and evaluation. Theriogenology, 20:407-416 https://doi.org/10.1016/0093-691X(83)90201-7
  10. Gordon I, Boland MP, MxGovern H and Lymm G. 1987. Effect of season on superovulatory responses and embryo quality in holstein cattle in Saudi Arabia. Theriogenology, 27:231 https://doi.org/10.1016/0093-691X(87)90108-7
  11. Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T and Machida T. 1990. A simple method, for mouse embryo cryopreservation in a low toxicity vitrification, without appreciable loss of viabillty. J. Reprod. Fertil., 89:91-97 https://doi.org/10.1530/jrf.0.0890091
  12. Katska L. 1984. Comparison of two methods for recovery of ovarian from slaughter cattle. Anim. Reprod. Sci., 7:461-463 https://doi.org/10.1016/0378-4320(84)90049-6
  13. Li R, Cameron AWN, Batt PA and Trounson AO. 1990. Maximum survival of frozen goat embryos is attained at the expanded, hatching and hatched blastocyst stages of development. Reprod. Fertil. Dev., 2:345-350 https://doi.org/10.1071/RD9900345
  14. Lu KH, Gorden I, Gallagher M and McGovern H. 1987. Pregnancy established in cattle by transfer of embryos derived from in vitro fertilization of oocytes matured in vitro. Vet. Rec., 121:259-260 https://doi.org/10.1136/vr.121.11.259
  15. Majumdar AC, Kharche SD, Tyagi S and Taru Sharma G. 1997. Effect of pretreatment with hCG and $estradiol-17\beta$ on superovulation and embryo recovery in goats. Theriogenology, 47: 176 https://doi.org/10.1016/S0093-691X(97)82303-5
  16. Massip A. 2001. Crypreservation of embryos of farm animals. Reprod. Domest. Anim., 36:49-55 https://doi.org/10.1046/j.1439-0531.2001.00248.x
  17. Nicholas FW. 1996. Genetic improvement through reproductive technology. Anim. Reprod. Sci., 42:205-214 https://doi.org/10.1016/0378-4320(96)01511-4
  18. Niemann H. 1991. Cryopreservation of ova and embryos from livestock: Current status and research need. Theriogenology, 35:109-124 https://doi.org/10.1016/0093-691X(91)90151-3
  19. Rho GJ, Hahnel AC and Betteridge KJ. 2001. Comparisons of oocyte maturation times and three methods of sperm preparation for their effects on the production of goat embryos in vitro. Theriogenology, 56:503-516 https://doi.org/10.1016/S0093-691X(01)00581-7
  20. Shea BF, Janzen RE and McDermand DP. 1984. Seasonal variation in response to stimulation and related embryo transfer procedures in Alberta over a nice year period. Theriogenology, 21:186-195 https://doi.org/10.1016/0093-691X(84)90316-9
  21. Smith C. 1986. Use of embryo transfer in genetic of sheep. Anim. Prod., 42:81-88 https://doi.org/10.1017/S000335610001775X
  22. Yoon WS, Lee CS, Goldman I, Fand NS, Koo DB, Han YM, Shin ST, Yoo OJ, Park CS and Lee KK. 1997. Studies on the superovulation and collection of microinjectable embryos in Korean native goat (Capra hircus aegagrus). Kor. J. Anim. Repr., 21:373-379
  23. Zarrow MX and Wilson ED. 1961. The influence of age on superovulation in the immature rat and mouse. Endocr., 69:851-855 https://doi.org/10.1210/endo-69-4-851