Journal of Microbiology

  • 제42권2호
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  • Pages.139-142
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  • 2004
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Isolation of Citrobacter sp. Mutants Defective in Decolorization of Brilliant Green by Transposon Mutagenesis

  • Jang, Moon-Sun (Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Young-Mi (Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Park, Yong-Lark (Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Cho, Young-Su (Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University) ;
  • Lee, Young-Choon (Faculty of Biotechnology, College of Natural Resources and Life Science, Dong-A University)
  • 발행 : 2004.06.01
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초록

To identify genes involved in the decolorization of brilliant green, we isolated random mutants generated by transposon insertion in brilliant green-decolorizing bacterium, Citrobacter sp. The resulting mutant bank yielded 19 mutants with a complete defect in terms of the brilliant green color removing ability. Southern hybridization with a Tn5 fragment as a probe showed a single hybridized band in 7 mutants and these mutants appeared to have insertions at different sites of the chromosome. Tn5-inserted genes were isolated and the DNA sequence flanking Tn5 was determined. By comparing these with a sequence database, putative protein products encoded by bg genes were identified as follows: bg 3 as a LysR-type regulatory protein; bg 11 as a MalG protein in the maltose transport system; bg 14 as an oxidoreductase; and bg 17 as an ABC transporter. The sequences deduced from the three bg genes, bg 2, bg 7 and bg 16, showed no significant similarity to any protein with a known function, suggesting that these three bg genes may encode unidentified proteins responsible for the decolorization of brilliant green.

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