Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Factors Influencing Preferential Utilization of RNA Polymerase Containing Sigma-38 in Stationary-Phase Gene Expression in Escherichia coli

  • Kim, Eun-Young (Genome Research Center for Enteropathogenic Bacteria, and Dept. of Microbiology, Chonnam National University Medical College) ;
  • Shin, Min-Sang (Genome Research Center for Enteropathogenic Bacteria, and Dept. of Microbiology, Chonnam National University Medical Colleg) ;
  • Rhee, Joon-Haeng (Genome Research Center for Enteropathogenic Bacteria, and Dept. of Microbiology, Chonnam National University Medical Colleg) ;
  • Hyon E. Choy (Genome Research Center for Enteropathogenic Bacteria, and Dept. of Microbiology, Chonnam National University Medical College)
  • Published : 2004.06.01
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Abstract

In order to understand the molecular basis of selective expression of stationary-phase genes by RNA polymerase containing$\sigma$$\^$38/ (E$\sigma$$\^$38/) in Escherichia coli, we examined transcription from the stationary-phase promoters, katEP, bo1AP, hdeABP, csgBAP, and mcbP, in vivo and in vitro. Although these pro-moters are preferentially recognized in vivo by E$\sigma$$\^$38/, they are transcribed in vitro by both E$\sigma$$\^$38/ and E$\sigma$$\^$70/ containing the major exponential $\sigma$, $\sigma$$\^$70/. In the presence of high concentrations of glutamate salts, how-ever, oldy E$\sigma$$\^$38/ was able to efficiently transcribe from these promoters, which supports the concept that the promoter selectivity of $\sigma$$\^$38/-containing RNA polymerase is observed only under specific reaction con-ditions. The examination of 6S RNA, which is encoded by the ssr1 gene in vivo, showed that it reduced E$\sigma$$\^$70/ activity during the stationary phase, but this reduction of activity did not result in the elevation of E$\sigma$$\^$38/ activity. Thus, the preferential expression of stationary-phase genes by E$\sigma$$\^$38/ is unlikely the con-sequence of selective inhibition of E$\sigma$$\^$70/ by 6S RNA.

Keywords

References

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