Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characteristics of Polyhydroxyalkanoates Producing Pseudomonas sp. MBEL21

신규 Pseudomonas sp. MBEL21 균주의 Polyhydroxyalkanoates 생산 특성

  • 최종일 (한국과학기술원 생명화학공학과, 생물공정연구센터 및 대사공학 국가지정연구실) ;
  • 이승환 (한국과학기술원 생명화학공학과, 생물공정연구센터 및 대사공학 국가지정연구) ;
  • 이상엽 (한국과학기술원 생명화학공학과, 생물공정연구센터 및 대사공학 국가지정연구실, 한국과학기술원 바이오시스템학과)
  • Published : 2004.06.01
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Abstract

Pseudomonas sp. MBEL21 was newly isolated from soil samples and found to accumulate medium-chain-length Polyhydroxyalkanoates(MCL-PHAs) using oleic acid as a sole carbon source. Among the various nutrient limiting conditions examined, including nitrogen, sulfur and phosphorus, only phosphorus limitation supported the accumulation of MCL-PHAs up to 15 wt％ of dry cell weight in flask cultures. MCL-PHAs produced by Pseudomonas sp. MBEL21 was mainly composed of 3-hydroxy-5-cis-tetradecenoate. Fed-batch culture of Pseudomonas sp. MBEL21 by novel feeding strategies based on cell growth charcteristics was carried out under phosphorus limitation using oleic acid as a sole carbon source. The final cell concentration and PHA content of 82 g/L and 28 wt％, respectively, were obtained. Furthermore, PHA consisted of MCL-hydroxyalkanoates and 3-hydroxybutyrate could be produced using olive oil as a sole carbon source.

토양으로부터 oleic acid 를 탄소원으로 이용하여 MCL-PHA의 생산능이 우수한 균주를 분리하여 Pseudomonas sp. MBEL21이라 명명하였다. Pseudomonas sp. MBEL21의 PHA함량을 증가시키기 위하여 여러 가지 필수 영양분의 제한 조건에서 배양한 결과, 인산이 제한된 조건에서 가장 높은 함량의 PHA를 축적하였다. 고농도의 MCL-PHA생산을 위한 Pseudomonas sp. MBEL21의 유가식 배양 전략을 개발하여 28.1 wt％의 함량으로 23 g/L. MCL-PHA를 생산하였다. 또한, 분리된 Pseudomonas sp. MBEL21을 olive oil을 탄소원으로 이용하여 배양한 결과 9.3 wt％의 PHA가 축적되어졌으며, MCL-hydroxyalkanoate와 함께 3-hyoxy-butyrate 의 단량체들로 이루어진 PHA가 확인되어졌다. 이러한 결과들은 분리된 신규 Pseudomonas sp. MBEL21이 저가의 olive oil로부터 생분해성 탄성체의 응용분야를 갖는 MCL-PHA를 효율적으로 생산할 수 있다는 것을 보여준다.

Keywords

References

  1. Int J Biol Macromol. v.16 Biosynthesis from gluconate of a random copolyester consisting of 3-hydroxybutyrate and medium-chain-length 3-hydroxyal-kanoates by Pseudomonas sp. 61-3 Abe, H.;Y. Doi;T. Fukushima;H. Eya https://doi.org/10.1016/0141-8130(94)90036-1
  2. Eur. J. Appl. Microbiol. Biotechnol. v.6 A rapid gas chromatographic method for the determination of poly-3-hydroxybutyric acid in microbial biomass Braunegg, G.;B. Sonnleitner;R. M. Lafferty https://doi.org/10.1007/BF00500854
  3. J. Environ. Poly. Degrad. v.5 Synthesis of poly(hydroxyalkanoate) from hydrolyzed linseed oil. Casini, E.;T. C. de Rijk;P. de Waard;G. Eggink
  4. Microbial polyesters Doi, Y.
  5. Industrial Crops and Products v.1 Oleic acid as a substrate for poly-3-hydroxyal-kanoate formation in Alcaligenes eutrophus and Pseudomonas putida Eggink, G.;H. vander Wal;G. N. M. Huijberts;P. de Waard https://doi.org/10.1016/0926-6690(92)90014-M
  6. Developments in crystalline polymers v.2 Biologically produced PHA polymers and copolymers Holmes, P. A.;D. C. Bassett(ed.)
  7. Appl. Microbiol. Biotechnol. v.46 Production of poly(3-hydroxyalkanoates) by Pseudomonas putida KT2442 in continuous cultures Huijberts, G. N. M.;G. Eggink https://doi.org/10.1007/s002530050810
  8. Enzyme Microb. Technol. v.24 High cell density fermentations of Pseudomonas oleovorans for the production of mcl-PHAs in two-liquid phase media Kellerhals, M. B.;W. Hazenberg;B. Witholt https://doi.org/10.1016/S0141-0229(98)00113-6
  9. Enzyme Microb. Technol. v.20 Enhanced yield and a high production of medium-chain-length poly(3-hydroxyalkanoates) in a two-step fedbatch cultivation of Pseudomonas putida by combined use of glucose and octanoate Kim, G. J.;I. Y. Lee;S. C. Yoon;Y. C. Shin;Y. H. Park https://doi.org/10.1016/S0141-0229(96)00179-2
  10. Biotechnol. Bioeng. v.67 Production of poly(3-hydroxybutyrate-co-3-hydroxy-hexanoate) by high cell density cultivation of Aeromonas hydrophila Lee, S. H.;D. H. Oh;W. S. Ahn;Y. Lee;J. Choi;S. Y. Lee https://doi.org/10.1002/(SICI)1097-0290(20000120)67:2<240::AID-BIT14>3.0.CO;2-F
  11. Trends Biotechnol. v.14 Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria Lee,S. Y. https://doi.org/10.1016/0167-7799(96)10061-5
  12. Biotechnol. Bioeng. v.49 Bacterial polyhydroxyalkanoates Lee, S. Y. https://doi.org/10.1002/(SICI)1097-0290(19960105)49:1<1::AID-BIT1>3.0.CO;2-P
  13. Biotechnol. Bioprocess Eng. v.1 High cell density cultivation of Pseudomonas oleovorans for the production of poly(3-hydroxyal-kanoates) Lee, S. Y. https://doi.org/10.1007/BF02949145
  14. Biotechnol. Bioeng. v.68 Production of Medium-chain-length polyhy-droxyalkanoates by high-cell-density cultivation of Pseudomonas putida under phosphorus limitations Lee, S. Y.;H. H. Wong;J. Choi;S. H. Lee;S. C. Lee;C. S. Han https://doi.org/10.1002/(SICI)1097-0290(20000520)68:4<466::AID-BIT12>3.0.CO;2-T
  15. Biotechnol. Bioprocess Eng. v.4 Fed-batch culture of Aeromonas hydrophila for the production of poly(3-hydroxy-butyrate-co-3-hydroxyhexanoate) using two carbon sources Lee, Y.;S. H. Lee;S. Y. Lee https://doi.org/10.1007/BF02931928
  16. Ann. Inst. Pasteur v.41 Products of dehydration and of poly-merization of β-hydroxybutyric acid Lemoigen, M.
  17. Macromolecules v.23 Physical characteristics of poly(3-hydroxyalkanoates) and poly (3-hydroxyalkanoates) produced by Pseudomonas oleovorans grwon on aliphatic hydrocarbons Preusting, H.;A. Nijenhuis;B. Witholt https://doi.org/10.1021/ma00221a007
  18. Biotechnol. Bioeng. v.41 High cell density cultivation of Pseudomonas oleovorans: Growth and production of poly(3-hydroxyalkanoates) in two liquid phase batch and fed-batch systems Preusting ,H.;R. van Houten;A. Hoefs;E. van Langenberghe;O. Faver-Bulle;B. Witholt https://doi.org/10.1002/bit.260410507
  19. Appl. Environ. Microbiol. v.57 Continuous production of long-side-chain poly-β-hydroxyalkanoates by Pseudomonas oleovorans Ramsay, B. A.;I. Saracovan;J. A. Ramsay;R. H. Marchessalt
  20. Biotechnol. Bioeng. v.55 Production of Poly(3-hydroxybutyrate) by high cell density fed-batch culture of Alcaligenes eutrophus with phosphate limitation Ryu, H. W.;S. K. Hahn;Y. K. Chang;H. N. Chang https://doi.org/10.1002/(SICI)1097-0290(19970705)55:1<28::AID-BIT4>3.0.CO;2-Z
  21. Trends Biotechnol. v.16 Bacterial and other biological systems for polyester production Steinbuchel, A.;B. Fuchtenbusch https://doi.org/10.1016/S0167-7799(98)01194-9
  22. FEMS Microbiol. Lett. v.128 Diversity of bacterial polyhydroxyalkanoic acid Steinbuchel, A.;H. E. Valentin https://doi.org/10.1111/j.1574-6968.1995.tb07528.x
  23. Appl. Environ. Microbiol. v.56 Formation of polyesters consisting of medium-chain-length 3-hydroxyalkanoic acids from gluconate by Pseudomonas aeruginosa and oter fluorescent pseudomonads Timm, A.;A. Steinbuchel