Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Bacteriocin Produced by Lactobacillus bulgaricus

  • Kim, Hyun-Jin (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Kim, Ji-Hyun (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Son, Jeong-Hwa (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Seo, Hyo-Jin (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Park, So-Jin (Department of Biotechnology and Bioengineering, Pukyong National University) ;
  • Paek, Nam-Soo (Mediogen Corp.) ;
  • Kim, Sung-Koo (Department of Biotechnology and Bioengineering, Pukyong National University)
  • Published : 2004.06.01
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Abstract

The antimicrobial substance produced by Lactobacillus bulgaricus was inactivated by protease. It showed inhibitory activity against Staphylococcus aureus ATCC6538, Streptococcus agalactiae ATCC14364, some Gram-positive and Gram-negative bacteria, and characteristics of a bacteriocin. The optimal temperature and culture time for the production of bacteriocin were $30^{\circ}C$ and 10 h, respectively, in the culture of L. bulgaricus. The bacteriocin production started in the exponential phase and reached a maximum at the early stationary phase. Using Staph. aureus ATCC6538 and Strep. agalactiae ATCC14364, known as common bovine mastitis pathogens, as indicator strains for determination of the bacteriocin activity, the antimicrobial activity of the bacteriocin was found to be stable in acidic and neutral pH's (2- 7) even at lOOT, whereas it was lost at high pH (10- 11) and $100^{\circ}C$. The mode of action for the antimicrobial activity was bacteriocidal, and the molecular weight determined by SDS-PAGE and overlay method was 14 kDa.

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References

  1. J. Microbiol. Biotechnol. v.6 Isolation of Leuconostoc mesenteroides subsp. mesenteroides DU-0608 with antibacterial activity from kimchi and Characterization of its bacteriocin Cha,D.S.;D.M.Ha
  2. J. Biotechnol. v.95 Influence of growth conditions on the production of a nisin-like bacteriocin by Lactococcus lactis subsp. lactis A164 isolated from kimchi Cheigh,C.I.;H.J.Choi;H.Park;S.B.Kim;M.C.Kook;T.S.Kim;J.K.Hwang;Y.R.Pyun https://doi.org/10.1016/S0168-1656(02)00010-X
  3. Food Microbiol. v.15 Comparative antimicrobial activity of enterocin L50, pediocin PA-1, nisin A and lactocin S against spoilage and foodborne pathogenic bacteria Chintas,L.M.;P.Casaus;M.F.Fernandez;P.E.Hernandez https://doi.org/10.1006/fmic.1997.0160
  4. J. Microbiol. Biotechnol. v.8 Inhibition of lactic acid bacteria in kimchi fermentation by nisin Choi,M.H.;Y.H.Park
  5. J. Dairy Res. v.47 Prevalence of different species of coagulase-negative staphylococci on teats and in milk samples from dairy cows Devriese,L.A.;H.D.Keyser https://doi.org/10.1017/S0022029900020999
  6. Am. J. Vet. Res. v.52 Effects on post milking teat on the colonization of S. aureus in chapped teat skin Fox,L.K.;J.A.Nagy;J.K.Hiller;J.D.Cronrath;D.A.Ratkowsky
  7. J. Microbiol. Biotechnol. v.4 Identification of bacteriocin-producing lactic acid bacteria from kimchi and partial characterization of their bacteriocin Ha,D.M.;D.S.Cha;S.G.Han
  8. Microbiol. Rev. v.59 Bacteriocins of gram-positive bacteria Jack,R.W.;R.T.John;R.Bibek
  9. J. Dairy Sci. v.5 Economic losses resulting from mastitis Janzen,J.J.
  10. Int. J. Food Microbiol. v.71 Bacteriocins: Safe, natural antimicrobials for food preservation Jennifer,C.;J.M.Thomas;F.N.Ingolf;L.C.Michael https://doi.org/10.1016/S0168-1605(01)00560-8
  11. J. Microbiol. Biotechnol. v.6 Evaluation of optimum conditions for bacteriocin production from Lactococcus sp. JB-42 isolated from kimchi Jo,Y.B.;K.M.Bae;S.K.Kim;H.K.Jun
  12. Kor. J. Vet. Res. v.41 Identification and antimicrobial susceptibility of microorganisms isolated from bovine mastitis milk Kang,H.J.;I.C.Kim;j.h.kIM;W.G.Son;D.S.Lee
  13. Food Sci. Biotechnol. v.9 Purification and molecular characterization of a bacteriocin from Pediococcus sp. KCA1303-10 isolated from fermented flatfish Kim,C.H.;G.E.Ji;C.Ahn
  14. Kor. J. Vet. Publ. Hlth. v.16 Patterns of mastitic pathogens and antibiotic susceptibility in milk of dairy cattle Kim,J.M.;S.C.Jung;J.Y.Lee;O.S.Seo;J.M.Park
  15. Food Sci. Biotechnol. v.9 Bacteriocin production by Lactococcus lactis KCA2386 isolated from white kimchi Ko,S.H.;C.Ahn
  16. Appl. Microbiol. Biotechnol. v.45 Lantibiotic nisin Z fermentative production by Lactococcus lactics IO: Relationship between production of the lantibiotic and lactate and cell growth Matsusaki,H.;N.Endo;K.Sonomoto;A.Ishizaki https://doi.org/10.1007/s002530050645
  17. Int. J. Food Microbiol. v.64 Detection and preliminary characterization of a bacteriocin (plantaricin 35d) produced by a Lactobacillus plantarum strain Messi,P.;M.Bondi;C.Sabia;R.Battini;G.Manicardi https://doi.org/10.1016/S0168-1605(00)00419-0
  18. J. Microbiol. Biotechnol. v.12 Synergistic effects of bacteriocin-producing Pediococcus acidilactici K10 and organic acids on inhibiting Escherichia coli O157:H7 and applications in ground beed Moon,G.S.;W.J.Kim;M.Kim
  19. J. Appl. Microbiol. v.88 production of sakacin P by Lactobacillus sakei in a completely defines medium Moretro,T.;I.M.Aasen;I.Storro;L.Axelsson https://doi.org/10.1046/j.1365-2672.2000.00994.x
  20. Eur. Food Chem. Proc. v.1 Bacteriocin Production by a Lactobacillus strain isolated from fermented meat Mortvedt,C.I.;I.F.Nes
  21. Int. J. Food Microbiol. v.70 Nutritional factors affecting the production of two bacteriocins from lactic acid bacteria on whey Nelson,P.G.;M.L.Rua;P.Lorenzo https://doi.org/10.1016/S0168-1605(01)00551-7
  22. J. Microbiol. Biotechnol. v.13 Purification and characterization of an antilisterial bacteriocin produced by Leuconostoc sp. W65 Oh,S.;M.Kim;J.J.Churey;R.W.Worobo
  23. Arch. Microbiol. v.154 Casein 80: Purification and characterization of a new bacteriocin from Lactobacillus casei Rammelsberg,M.;E.Muller;F.Radler https://doi.org/10.1007/BF00248963
  24. Int. J. Food Microbiol. v.80 Heterologous production of bacteriocins by lactic acid bacteria Rodriguez,J.M.;M.I.Martinez;N.Horn;H.M.Dodd https://doi.org/10.1016/S0168-1605(02)00153-8