Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea

  • Guk, Sang-Mee (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Yong, Tai-Soon (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Park, Soon-Jung (Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine) ;
  • Park, Jae-Hwan (Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Chai, Jong-Yil ( Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
  • Published : 2004.06.01
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Abstract

Cryptosporidium parvum oocysts were isolated from a child suffering from acute gastroenteritis and successfully passaged in a calf and mice (designated hereafter SNU-H1) in the Republic of Korea; its molecular genotype has been analyzed. The GAG microsatellite region was amplified by a polymerase chain reaction (PCR), with a 238 base pair product, which is commonly displayed in C. parvum. The isolate was shown to be a mixture of the genotypes 1 (anthroponotic) and 2 (zoonotic). To study its infectivity in animals, 2 calves and 3 strains of mice were infected with the SNU-H1; in these animals, the propagation of both genotypes was successful. In immunosup-pressed (ImSP) BALB/c and C57BU6 mice the number of oocysts decreased after day 10 post-infection (PI); but in ImSP ICR mice, they remained constant until day 27 PI. The results show that both the C. parvum genotypes 1 and 2 can be propagated in calves and ImSP mice.

Keywords

References

  1. Akiyoshi DE, Feng X, Buckholt MA, Widmer G, Tzipori S (2002) Genetic analysis of a Cryptosporidium parvum human genotype 1 isolate passaged through different host species. Infect Immun 70: 5670-5675 https://doi.org/10.1128/IAI.70.10.5670-5675.2002
  2. Caccio S, Homan W, Camilli R, Traldi G, Kortbeek T, Pozio E (2000) A microsatellite marker reveals population heterogeneity within human and animal genotypes of Cryptosporidium parvum. Parasitology 120: 237-244
  3. Chai JY, Shin SM, Yun CK, Yu JR, Lee SH (1990) Experimental activation of cryptosporidiosis in mice by immunosuppression. Korean J Parasitol 28: 31-37 https://doi.org/10.3347/kjp.1990.28.1.31
  4. Chai JY, Lee SH, Guk SM, Lee SH (1996) An epidemiological survey of Cryptosporidium parvum infection in randomly selected inhabitants of Seoul and Chollanam-do. Korean J Parasitol 34: 113-119 https://doi.org/10.3347/kjp.1996.34.2.113
  5. Chai JY, Guk SM, Han HK, Yun CK (1999) Role of intraepithelial lymphocytes in mucosal immune responses of mice experimentally infected with Cryptosporidium parvum. J Parasitol 85: 234-239 https://doi.org/10.2307/3285625
  6. Chai JY, Kim NY, Guk SM, et al. (2001) High prevalence and seasonality of cryptosporidiosis in a small rural village occupied predominantly by aged people in the Republic of Korea. Am J Trop Med Hyg 65: 518-522
  7. Fayer R, Ungar BLP (1986) Cryptosporidium spp. and cryptosporidiosis. Microbiol Rev 50: 458-483
  8. Feng X, Rich SM, Tzipori S, Widmer G (2002) Experimental evidence for genetic recombination in the opportunistic pathogen Cryptosporidium parvum. Mol Biocheml Parasitol 119: 55-62
  9. Giles M, Webster KA, Marshall JA, Catchpole J, Goddard TM (2001) Experimental infection of a lamb with Cryptosporidium parvum genotype 1. Vet Rec 149: 523-525
  10. Guk SM, Yong TS, Chai JY (2003) Role of murine intestinal intraepithelial lymphocytes and lamina propria lymphocytes against primary and challenge infections with Cryptosporidium parvum. J Parasitol 89: 270-275
  11. McLauchlin J, Amar C, Pedraza-Diaz S, Nichols GL (2000) Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in 1,705 fecal samples from humans and 105 fecal samples from livestock animals. J Clin Microbiol 38: 3984-3990
  12. Morgan UM, Constantine CC, Forbes DA, Thompson RCA (1997) Differentiation between human and animal isolates of Cryptosporidium parvum using a rDNA sequencing and direct PCR analysis. J Parasitol 83: 825-830 https://doi.org/10.2307/3284275
  13. Morgan UM, Fall A, Ward LA, et al. (2002) Cryptosporidium hominis n. sp. (Apicomplexa: Cryptosporidiidae) from Homo sapiens. J Eukaryot Microbiol 49: 433-440 https://doi.org/10.1111/j.1550-7408.2002.tb00224.x
  14. Morgan UM, Xiao L, Hill BD, et al. (2000) Detection of the Cryptosporidium parvum “Human” genotype in Dugong (Dugong dugon). J Parasitol 86: 1352-1354
  15. Spano F, Putignani L, Crisanti A, et al. (1998) Multilocus genotypic analysis of Cryptosporidium parvum isolates from different hosts and geographical origins. J Clin Microbiol 36: 3255-3259
  16. Widmer G, Akiyoshi D, Buckholt MA, et al. (2000) Animal propagation and genomic survey of a genotype 1 isolate of Cryptosporidium parvum. Mol Biochem Parasitol 108: 187-197
  17. Widmer G, Lin L, Kapur V, Feng X, Abrahamsen MS (2002) Genomics and genetics of Cryptosporidium parvum: the key to understanding cryptosporidiosis. Microbes Infect 4: 1081-1090
  18. Xiao L, Bern C, Limor J, et al. (2001) Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J Infect Dis 183: 492-497
  19. Yu JR, Lee JK, Seo M, et al. (2004) Prevalence of cryptosporidiosis among the villagers and domestic animals in several rural areas of Korea. Korean J Parasitol 42: 1-6