Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Immunolocalization of the 150 kDa protein in cyst fluid of Taenia solium metacestodes

  • Yang, Hyun-Jong (Department of Parasitology, College of Medicine, Ewha Womans University) ;
  • Chung, Young-Bae (Department of Parasitology, College of Medicine and Institute of Medical Science, Cheju National University)
  • Published : 2004.06.01
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Abstract

The 150 kDa protein of cyst fluid (CF) of Taenia solium metacestodes was purified by ammonium sulfate fractionation and Superose 6 HR gel filtration chromatography. The purified protein consisted of three subunits (15, 10 and 7 kDa proteins), which were analyzed with the use of a 7.5-15％ gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SOS-PAGE). Immunofluorescence study was carried out by using immunize specific polyclonal antibody. Positive reactions were noticed at bladder walls, calcareous corpuscles, granules of cyst fluid and some host tissue surrounding the bladder wall of the metacestodes. These results suggest that the 150 kDa protein was secreted into host tissues, inducing immune responses in the host, and it may play important roles in the cellular physiology of the parasites.

Keywords

References

  1. Cho SY, Kim SI, Kang SY, Kong Y (1988) Biochemical properties of a purified protein of Taenia solium metacestodes. Korean J Parasitol 26: 87-94 https://doi.org/10.3347/kjp.1988.26.2.87
  2. Chung JY, Bahk YY, Huh S, Kang SY, Kong Y, Cho SY (1999) A recombinant 10-kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. J Infect Dis 180: 1307-1315 https://doi.org/10.1086/315020
  3. Chung JY, Yun DH, Eom KS, Kang SY, Kong Y, Cho SY (2002) Taenia solium: identification of specific antibody binding regions of metacestode 10-kDa protein. Exp Parasitol 100: 87-94
  4. Chung YB, Lee M, Yang HJ, et al. (2002) Characterization of partially purified 8 kDa antigenic protein of Clonorchis sinensis. Korean J Parasitol 40: 83-88
  5. Del Brutto OH, Rajshekhar V, White AC Jr, et al. (2001) Proposed diagnostic criteria for neurocysticercosis. Neurology 57: 177-183
  6. Laclette JP, Merchant MT, Wilms K (1987) Histological and ultrastructural localization of antigen B in the metacestode of Taenia solium. J Parasitol 73: 121-129 https://doi.org/10.2307/3282356
  7. Pawlowski ID, Yap KW, Thompson RC (1988) Observation on the possible origin, formation and structure of calcareous corpuscles in taeniid cestodes. Parasitol Res 74: 293-296 https://doi.org/10.1007/BF00539581
  8. Tsang VC, Brand JA, Boyer AE (1989) An enzyme-linked immunoelectrotransfer blot and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159: 50-59 https://doi.org/10.1093/infdis/159.1.50
  9. Yang HJ, Chung JY, Yun D, et al. (1998) Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes. Parasite Immunol 20: 483-488 https://doi.org/10.1046/j.1365-3024.1998.00179.x