한국초지조사료학회지 (Journal of The Korean Society of Grassland and Forage Science)

  • 제24권1호
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  • Pages.29-36
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  • 2004
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  • 2287-5824(pISSN)
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  • 2287-5832(eISSN)
한국초지조사료학회 (The Korean Society of Grassland and Forage Science)
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들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

Factors Affecting Callus Induction and Plant Regeneration from Mature Seed of Zoysiagrass (Zoysia japonica Steud.)

  • 이상훈 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 김범수 (경상대학교 응용생명과학부, 농업생명과학연구원) ;
  • 원성혜 (경북대학교 농업생명과학대학) ;
  • 조진기 (경북대학교 농업생명과학대학) ;
  • 김기용 (축산기술연구소) ;
  • 박근제 (축산기술연구소) ;
  • 성병렬 (축산기술연구소) ;
  • 이효신 (국립산림과학원) ;
  • 이병현 (경상대학교 응용생명과학부, 농업생명과학연구원)
  • 발행 : 2004.03.01
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초록

들잔디(Zoysia japonica Steud.)의 최적 조직 배양 조건을 확립하기 위하여 성숙종자로부터 최적 캘러스 유도 및 효율적인 식물체 재분화에 미치는 몇 가지 요인의 영향을 조사하였다. 성숙종자로부터 배발생 캘러스 유도율은 3mg/L 2,4-D가 첨가된 MS 배지에서 85%로 가장 높았으며 NAA 또는 IAA를 첨가한 처리구보다 높게 나타났다. 배발생 캘러스로부터 식물체 재분화는 0.1 mg/L 2,4-D와 5 mg/L BA가 첨가된 N6 배지에서 73.3%로 가장 높게 나타났다. 기본배지의 종류에 따른 배양효율의 차이는 배발생 캘러스 유도에는 MS 배지가, 식물체 재분화에는 N6배지가 가장 효과적이었다. 재분화 된 식물체는 1/2 MS rooting 배지에서 뿌리를 형성되어 pot에 이식하였을 때 정상적으로 분화되어 생장하였다. 본 연구를 통하여 확립된 단기간 고효율 재분화 시스템은 분자육종을 통한 신품종 들잔디의 개발에 유용하게 응용되어질 수 있을 것이다.

In an effort to optimize tissue culture responses of zoysiagrass(Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 mg/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 mg/L 2,4-D and 5 mg/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

키워드

참고문헌

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피인용 문헌

  1. Establishment of Callus Induction and Plant Regeneration System from Mature Seeds of Miscanthus sinensis vol.24, pp.5, 2011, https://doi.org/10.7732/kjpr.2011.24.5.628