YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effect of Pharbitidis Seed Extract on the Antioxidant Enzyme Activity in B16F10 Murine Melanoma Cells by Oxidative Stress

산화적 스트레스상태에서 B16F10 Murine Melanoma 세포의 항산화효소 활성에 대한 흑축추출물의 효과

  • 김안근 (숙명여자대학교 약학대학) ;
  • 차은정 (숙명여자대학교 약학대학)
  • Published : 2004.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

The purpose of this study was to evaluate the effect of pharbitidis seed extract (PE) on antioxidant enzymes. The cytotoxicities of PE were measured by 3- (4,5-dimethlthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; The change of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activity assay were measured. The SOD activities by PE-treated groups were lower than control group's one. In the co-treated with hydrogen peroxide ($H_2O$$_2$) group, SOD activity was higher than $H_2O$$_2$ treated group's activity In the case of GPx, GPx activities were increased in both PE-treated and co-treated with $H_2O$$_2$ group. In the case of CAT $H_2O$$_2$ treated group's activityies were very increased. The CAT activities by PE-treated groups were lower than control group's one, but the activity of co-treated group with H $_2$O$_2$ was higher than that of control group's one. These results suggest that PE has antioxidant activity.

Keywords

References

  1. Free Radical Biol. Med. v.19 Antioxidant enzyme levels as a function of growth state in cell culture Terry D. Oberley;Janice L. Schultz;Ning Li;Larry W. Oberley https://doi.org/10.1016/0891-5849(95)00012-M
  2. J. Dermatol Sci. v.14 Time course of changes in antioxidant enzymes in human skin fibroblasts after UV irradiation Yasuko, Shindo;Takashi, Hashimoto https://doi.org/10.1016/S0923-1811(96)00578-6
  3. J. Am. Acad. Dermatol. v.38 Protective effect against sunburn of combined systemic ascorbic acid (vitamin C) and a-tocopherol (vitamin E) Bernadette,E.K.;Marianne,D.;Bernhard,P. https://doi.org/10.1016/S0190-9622(98)70537-7
  4. Casarett and Doull's toxicology Mechanisms of toxicity Zoltan, Gregus;Curtis D. Klaassen
  5. Casarett and Doull's toxicology Toxic responses of the heart and vascular systems Kenneth S. Ranos;Enrique Chacon;Jr.Daniel Acosta
  6. British Journal of Nutrition v.84 The effect of vitamin C or vitamin E supplementation on basal and H₂O₂-induced DNA damage in human lymphocytes Lisa A. Brennan;Gerard M. Morris;Gillian R. Wasson;Bernadette M. Hannigan;Yvonne A. Barnett https://doi.org/10.1079/096582197388680
  7. Rev. Med. Chir. Soc. Med. Nat. Iasi. v.103 Oxidative stress and anti-oxidants in the diet in pathological processes at the level of the cardiovascular system Gavat,V.;Voroniuc,O.
  8. Free Radical Biol. Med. v.24 Alterations of anti-oxidant enzyme expression in response to hydrogen peroxide Elke, Rohrdanz;Regine, Kahl https://doi.org/10.1016/S0891-5849(97)00159-7
  9. Eur. J. Biochem. v.47 Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase Stefan, Marklund;Gudrun, Marklund https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  10. Anal. Biochem. v.137 The pyrogallol assay for superoxide dismutase: absence in glutathione artifact Eugene F. Roth,Jr.;Harriet S. Gilbert https://doi.org/10.1016/0003-2697(84)90344-0
  11. Methods in Enzymology v.105 Assays of glutathione peroxidase Flohe, Leopold;Wolfgang A. Gunzler https://doi.org/10.1016/S0076-6879(84)05013-8
  12. J. Lab. & Clin. Med. v.70 Studies on the qauntitative and qualitative characterization of erythrocyte glutathione peroxidase Donald E. Paglia;William N. Valentine
  13. Methods in Enzymology v.105 Catalase in vitro Hugo, Abei https://doi.org/10.1016/S0076-6879(84)05013-8