Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
권호 표지

Plant Regeneration from Leaf Segment Culture of Chrysanthemum zawadskii ssp. coreanum

한라구절초 잎절편 배양에 의한 식물체 재분화

  • Published : 2004.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

The effect of plant growth regulators and gelling agents for plant regeneration from leaf segment of Chrysanthemum zawadskii ssp. coreanum was investigated. NAA was more effective than BA for plant regeneration. MS medium supplemented with NAA 1 mg/L was the most effective in plant regeneration. The effect of agar and gelite as gelling agent was compared. Agar(0.8%) was more effective than gelite(0.2%) in plant regeneration. Regenerated shoots was successfully increased by shoot grafting in MS medium supplemented with NAA 0.1 mg/L in vitro, and hardened by shoot grafting in artificial soil mix(Peatmoss : Perlite = 1 : 1).

기내에서 삽목 배양중인 한라구절초[( Chrysanthemum zawadskii ssp. coreanum(Nakai) Y.N. Lee)]의 잎으로부터 효과적인 식물체 재생을 위한 NAA와 BA농도 및 배지고형물의 효과를 조사하였다. 0.2% Gelite를 고형물로 하였을 때 한라구절초의 잎 재분화에는 BA농도보다는 NAA 농도가 더 영향을 미쳤으며, NAA 농도가 1 mg/L 이상이면 모든 조직 에서 재분화를 보였다. 신초수는 NAA 단용 처리에서 많은 경향이었으며 NAA 1 mg/L 처리시가 가장 양호하였다. 배지고형물로 agar과 gelite를 가지고 재분화 효과를 조사한 결과, gelite 첨가시 잎절편 당 신초수가 많았으며 agar 첨가시에는 잎절편당 신초수가 적은 반면에 신초의 길이가 길게 나타났다. 한라구절초의 잎절편으로부터 재분화에는 MS에 3% sucrose, 0.8% agar와 NAA 1 mg/L를 첨가하여 25 $^{\circ}C$, 2,000 lux 정도에서 배양하는 것이 효과적이었다.

Keywords

References

  1. Plant Growth Reg. v.15 Polyamine metabolism and in vitro cell multiplication and differentiation in leaf explants of Chrysanthemum morifolium Ramat Arabaud,M.;M.Carre;J.Martin-Tanguy https://doi.org/10.1007/BF00024104
  2. Physiol. Mol. Plant Path. v.39 Cultivar-strain specificity between Chrysanthemum morifolium and Agrobacterium tumefaciens Bush,A.L.;S.G.Pueppke https://doi.org/10.1016/0885-5765(91)90038-J
  3. Amer. J. Bot. v.63 Plantlets from petal segments, petal epidermis, and shoot tips of the periclinal chimera, Chrysanthemum morifolium 'Indianapolis' Bush,S.L.;E.D.Earle;R.W.Langhans https://doi.org/10.2307/2442032
  4. Plant Cell Tiss. Org. Cult. v.21 Shoot regeneration from stem and leaf explants of Dendranthema grandiflora Tzvelev(syn. Chrysanthemum morifolium Ramat.) Kaul,V.;R.M.Miller;J.F.Hutchinson;D.Richards https://doi.org/10.1007/BF00034487
  5. Kor. J. Plant Tiss. Cult. v.26 no.1 Plant regeneration from leaf segment cultures of Chrysanthemum(Dendranthema gradiflora Tzvelev) Lee,Y.K.;Y.J.Kwon;K.M.Lee;N.I.Hyung
  6. Physiol. Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murashige,T.;F.Skoog https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  7. Ann. Bot. v.40 Abnormalities in Chrysanthermum regenerated from long term cultures Sutter,E.;R.W.Langhans