Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Molecular Characterization of Iridovirus in Cultured Turbot, Scophthazmus maximus

양식산 Tubot, Scophthalmus maximus 감염원으로 확인된 Iridovirus의 분자생물학적 연구

  • Published : 2004.02.01
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Abstract

The high mortality of cultured turbot, Scophthalmus maximus was occurred from Gochang in June, 2003. Infect fish with iridovirus showed a lower feed intake and lethargic. These fish exhibited pale body color, extended abdominal and exophthalmus. Histopathological studies showed basophilic enlarged cells from kidney, spleen, gill, heart, muscle, stomach, intestine, liver and pancreas of these fish. In PCR amplification with red sea bream iridovirus (RSIV) specific primer set of a DNA fragment of 286 bp was obtained from infect turbot with the virus. The strain showed the high homologies with RSIV, LBIV (largemouth bass iridovirus), GSIV (giant seaperch iridovirus) and SBIV (sea bass iridovirus).

2003년 6월과 7월, 고창에서 사육중인 turbot이 약 90% 누적폐사되었고, 주 증상은 안구돌출, 비장 비대 그리고 장내 내용물이 없는 것으로 관찰되었다. 병리조직학적 검사에서 RSIV와 유사한 이형비대세포가 모든 장기에서 관찰되어 RSIV검출을 위한 primer로 PCR을 실시하였다. 그 결과 PCR에서 특이밴드가 모든 조직에서 검출되어졌고, 염기서열 분석 결과 기존에 보고된 strain과 88%이상의 유사성을 나타내는 것으로 확인 되어져서 터봇의 페사 원인으로 이리도바이러스감염이 관여됨을 확인할 수 있었다.

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References

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