Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Induction of a Sweetpotato Anion Peroxidase swpa2 Gene Expression by Stress-related Chemicals and Pectobacterium chrysanthemi

스트레스 관련 화합물 처리 및 병원균 감염에 의한 고구마 산성 퍼옥시다제 swpa2 발현 유도

  • Kim, Yun-Hee (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Ryu, Sun-Hwa (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kim, Kee-Yeun (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Kwon, Suk-Yoon (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB)) ;
  • Bang, Jae-Wook (Department of Biology, Chungnam National University) ;
  • Kwak, Sang-Soo (Laboratory of Environmental Biotechnology, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
  • 김윤희 (한국생명공학연구원 환경생명공학연구실) ;
  • 류선화 (한국생명공학연구원 환경생명공학연구실) ;
  • 김기연 (한국생명공학연구원 환경생명공학연구실) ;
  • 권석윤 (한국생명공학연구원 환경생명공학연구실) ;
  • 방재욱 (충남대학교 생물학과) ;
  • 곽상수 (한국생명공학연구원 환경생명공학연구실)
  • Published : 2004.03.01
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Abstract

Expression of an anionic peroxidase swpa2 gene isolated from cultured cells of sweetpotato (Ipomoea batatas) was investigated under various stress conditions by RT-PCR. The swpa2 gene was not expressed in any tissues of intact sweetpotato plant grown at the normal condition. The expression of this gene was strongly induced in leaf tissue by treatment of $H_2O$$_2$ (440mM). Treatment of NaCl (100mM), ABA (0.1mM) and methyl jasmonate(MeJA, 0.1mM) also induced the expression of swpa2 gene. Interestingly, salicylic acid (SA, 0.1 mM) did not induce the expression of swpa2 gene, indicating that anionic swpa2 POD is differently involved in SA and MeJA signaling pathways. In addition, swpa2 gene was strongly induced in sweetpoato leaf tissues infected with Pectobacterium chrysanthemi, indicating that swpa2 is involved in defense related to the pathogenesis of P. chrysanthemi in sweetpotato plants. These results strongly suggest that swpa2 gene is involved in overcoming oxidative stresses caused by both abiotic and biotic stress.

스트레스 관련 화합물 및 병원균을 고구마 (Ipomoea batatas)잎에 처리하여 고구마 배양세포에서 분리한 산성 퍼옥시다제 swpa2 유전자의 발현을 RT-PCR로 분석하였다. Swpa2유전자는 건강한 식물체 조직에서는 발현되지 않았으나 각종 스트레스에 의해 발현이 유도되었다. Swpa2는 과산화수소 (440mM)처리에 의하여 강하게 발현이 유도되었고 NaCl (100mM), ABA (0.1mM), methyl jasmonate (MeJA, 0.1mM) 처리에 의하여도 발현이 유도되었다. 그러나 salicylic acid (SA)처리에 의해서는 발현이 유도되지 않아, 이 유전자는 SA와 MeJA의 신호전달에는 서로 다르게 관여함이 시사되었다. 또한 swpa2유전자는 무름병을 유발하는 박테리아(Pectobacterium chrysanthemii)의 감염에 의하여 강하게 발현되므로, 이 POD는 병저항성에 관여함을 알 수 있었다. 따라서 swpa2유전자는 각종 비생물학적 스트레스 뿐 아니라 생물학적 스트레스에 수반되는 산화 스트레스를 극복하는데 관여함이 강하게 제시되었다.

Keywords

References

  1. Baga M, Chibbar RN, Kartha KK (1995) Molecular cloning and expression analysis of peroxidase genes from wheat. Plant Mol Biol 29:647-662 https://doi.org/10.1007/BF00041156
  2. Choi SM, Lee OS, Kwon SY, Kwak SS, Yu DY, Lee HS (2003) High expression of a human lactoferrin in transgenic tobacco cell cultures. Biotech Lett 25: 213-218 https://doi.org/10.1023/A:1022341917735
  3. dark CA, Moyer JW (1988) Compendium of sweetpotato disease APS Press, St Paul. pp 74 truncatula precedes infection by Rhizobium meliloti. Plant Cell 7: 43-55 https://doi.org/10.1007/BF00272975
  4. Curtis MD, Rae AL, Rusu AG, Hanison SJ, Manners JM (1997) A peroxidase gene promoter induced by phytopathogens and methyl jasmonate in transgenic plants. Mol Plant Microb Interact 3: 326-338
  5. Doares SH, Narvaez-Vasquez J, Conconin A, Ryan CA (1995) Salicylic acid inhibits synthesis of proteinase inhibitors in tomato leaves induced by systemin and jasmonic acid. Plant Physiol 108: 1741-1746
  6. Felton GW, Donato K, Delvecchio RJ, Duffey SS (1989) Activation of plant foliar oxidase by insect feeding reduces nutritive quality of foliage for noctuid herbivores. J Chem Ecol 15: 2667-2694 https://doi.org/10.1007/BF01014725
  7. Gazarian IG, Lagrimini LM, Gillian AA, Thorneley NF (1996) Mechanism of indole-3-acetic acid oxidation by plant peroxidase: anaerobic stopped-flow spectrophotometric studies on horseradish and tobacco peroxidases. Biochem J 313: 841-847
  8. Hiraga S, Yamamoto K, Ito H, Sasaki K, Matsui H, Honma M, Nagamura Y, Sasaki T, Ohashi Y (2000) Diverse expression profiles of 21 rice peroxidase genes. FEBS Lett 471: 245-250 https://doi.org/10.1016/S0014-5793(00)01409-5
  9. Hiraga S, Sasaki K, Ito H, Ohashi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol 42: 462-468 https://doi.org/10.1093/pcp/pce061
  10. Huh GH, Lee SJ, Bae YS, Liu JR, Kwak SS (1997) Molecular cloning and characterization of cDNAs for anionic and neutral peroxidase from suspension cultured cells of sweetpotato and their differential expression in response to stress. Mol Gen Genet 255: 382-391 https://doi.org/10.1007/s004380050510
  11. Kim KY, Huh GH, Lee HS, Kwon SY, Hur Y, Kwak SS (1999) Molecular characterization of cDNAs for two anionic peroxidases from suspension cultures of sweetpotato. Mol Gen Genet 261: 941-947 https://doi.org/10.1007/s004380051041
  12. Kim KY, Kwon HK, Kwon SY, Lee HS, Hur Y, Bang JW, Choi KS, Kwak SS (2000) Differential expression of four sweet potato peroxidase genes in response to abscisic acid and ethephon. Phytochemistry 54:19-22 https://doi.org/10.1016/S0031-9422(00)00014-5
  13. Kim KY, Kwon SY, Lee HS, Hur Y, Bang JW, Kwak SS (2003) A novel oxidative stress-inducible peroxidase promoter from sweetpotato: molecular cloning and characterization in transgenic tobacco plants and cultured cells. Plant Mol Biol 51:831-838 https://doi.org/10.1023/A:1023045218815
  14. Kim SK, Kwak SS, Jung KH, Min SR, Park IH, Liu JR (1994) Selection of plant cell lines for high yields of peroxidase. J Biochem Mol Biol 27:132-137
  15. Klotz KL, Liu TT, Liu L, Lagrimini LM (1998) Expression of the tobacco anionic peroxidase gene is tissue-specific and developmentally regulated. Plant Mol Biol 36: 509-520 https://doi.org/10.1023/A:1005939600344
  16. Kunkel BN, Brooks DM (2002) Cross talk between signalling pathways in pathogen defense. Curr Opin Plant Biol 5:325-331 https://doi.org/10.1016/S1369-5266(02)00275-3
  17. Kwak SS, Kim SK, Lee MS, Jung KH, Park IH, Liu JR (1995) Acidic peroxidases from suspension-cultures of sweetpotato. Phytochemistry 39: 981-984 https://doi.org/10.1016/0031-9422(95)00098-R
  18. Kwon SY, Choi SM, Lee OS, Kwak SS, Lee HS (2003) Transgenic ginseng cell lines that produce high levels of a human lactofemn. Planta Med 69:1005-1008 https://doi.org/10.1055/s-2003-45146
  19. Lagnrnini LM (1996) The role of the tobacco of anionic peroxidase in growth and development, In: Obinger C, Bumer U, Ebermann R, Penel C, Greppin H, (eds), Plant Peroxidase: Biochemistry and Physiology, University of Agriculture, Vienna and University of Geneva, pp 235-242
  20. Linsmaier EM, Skoog F (1965) Organic growth factor requirements of tobacco tissue cultures. Physiol Plant 18: 100-127 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
  21. Mader M, Fussl DF (1982) Role of peroxidase in lignification of tobacco cells. Plant Physiol 70:1132-1134 https://doi.org/10.1104/pp.70.4.1132
  22. Naqvi SM, Park KS, Yi SY, Lee HW, Bok SH, Choi DI (1998) A glycine-rich RNA-binding protein gene is differentially expressed during acute hypersensitive response following tobacco mosaic virus infection in tobacco. Plant Mol Biol 37: 571-576 https://doi.org/10.1023/A:1006031316476
  23. Neil S, Desikan R, Hancock J (2002) Hydrogen peroxide signalling. Curr Opin Plant Biol 5: 388-395 https://doi.org/10.1016/S1369-5266(02)00282-0
  24. Niki T, Mitsuhara I, Seo S, Ohtsubu N, Ohashi Y (1998) Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) Protein genes in wounded mature tobacco leaves. Plant Cell Physiol 39: 500-507 https://doi.org/10.1093/oxfordjournals.pcp.a029397
  25. Omann F, Beauliew N, Tyson H (1993) cDNA sequence and tissue specific expression of an anionic peroxidase. Genome 37: 137-147 https://doi.org/10.1139/g94-018
  26. Park SY, Ryu SH, Kwon SY, Lee HS, Kim JG, Kwak SS (2003a) Molecular cloning and characterization of six peroxidase cDNAs from cell cultures of sweetpotato and their differential expressions in response to stress. Mol Genet Genomics 269: 542-552 https://doi.org/10.1007/s00438-003-0862-y
  27. Park SY, Ryu SH, Kwon SY, Kim JG, Kwak SS (2003b) Molecular cloning and characterization of a peroxiredoxin cDNA from cell cultures of sweetpotato. Korean J Plant Biotechnol 30:135-141 https://doi.org/10.5010/JPB.2003.30.2.135
  28. Park SY, Ryu SH, Kwon SY, Kim JG, Kwak SS (2004) Molecular cloning of a cytosolic ascorbate from cell cultures of sweetpotato and its expression in response to stress. Mol Genet Genomics (in press)
  29. Roberts E, Kutchan T, Kolattukudy PE (1988) Cloning and sequencing of cDNA for a highly anionic peroxidase from potato and the induction of its mRNA in suberizing potato tubers and tomato fruits. Plant Mol Biol 11:15-26 https://doi.org/10.1007/BF00016010
  30. Schenk PM, Kazan K, Wilson I, Anderson JP, Richmond T, Somerville SC, Manners JM (2000) Coordinated plant defense responses in Arabidopsis revealed by microarray analysis. Proc Natl Acad Sci USA 97:11655-11660 https://doi.org/10.1073/pnas.97.21.11655
  31. Tognolli M, Penel C, Greppin H, Simon P (2002) Analysis and expression of the class III peroxidase large gene family in Arabidopsis thaliana. Gene 288:129-138 https://doi.org/10.1016/S0378-1119(02)00465-1
  32. Vignutelli A, Wastemack C, Apel K, Bohlmann H (1998) Systemic and local induction of an Arabidopsis thionin gene by wounding and pathogens. Plant J 14: 285-295 https://doi.org/10.1046/j.1365-313X.1998.00117.x
  33. Welinder KG (1992) Superfamily of plant, fungal, and bacterial peroxidases. Curr Opin Struct Biol 2: 388-393 https://doi.org/10.1016/0959-440X(92)90230-5
  34. Welinder KG, Justesen AF, Kjasrsgard IVH, Jensen RB, Rasmussen SK, Jespersen HM, Duroux L (2002) Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana. Eur J Biochem 269:6063-6081 https://doi.org/10.1046/j.1432-1033.2002.03311.x
  35. Xu Y, Chang PLC, Liu D, Narashimhan ML, Kashchandra GR, Hasegawa PM, Bressan RA (1994) Plant defense genes are synergistically induced by ethylene and methyl jasmonate. Plant Cell 6: 1077-1085 https://doi.org/10.1105/tpc.6.8.1077
  36. Ye XS, Pan SQ, Kuc J (1990) Activity, isozyme pattem, and cellular localization of peroxidase as related to systemic resistance of tobacco to blue mold (Peronospora tabacina) and to tobacco mosaic virus. Physiol Biochem 80:1295-1299
  37. Zimmerlin A, Wojtaszek P, Bolwell GP (1994) Synthesis of dehydrogenation polymers of ferulic with highly specificity by a purified cell wall peroxidase from French bean (Phaseolus uulgaris L.). Biochem J 299: 747-75

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