Journal of Plant Biotechnology

  • 제31권1호
  • /
  • Pages.43-48
  • /
  • 2004
  • /
  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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이탈리안 라이그래스의 성숙종자 유래 캘러스로부터 효율적인 식물체 재분화

Efficient Plant Regeneration from Mature Seed-derived Callus of Italian ryegrass (Lolium multiflorum Lam.)

  • 우현숙 (경상대학교 응용생명과학부) ;
  • 이상훈 (경상대학교 응용생명과학부) ;
  • 이동기 (경상대학교 응용생명과학부) ;
  • 김진수 (경상대학교 응용생명과학부) ;
  • 원성혜 (경북대학교 농업과학기술연구소) ;
  • 이병현 (경상대학교 PMBBRC)
  • Woo, Hyun-Sook (Division of Applide Life Science, Gyeongsang National University) ;
  • Lee, Sang-Hoon (Division of Applide Life Science, Gyeongsang National University) ;
  • Lee, Dong-Gi (Division of Applide Life Science, Gyeongsang National University) ;
  • Kim, Jin-Soo (Division of Applide Life Science, Gyeongsang National University) ;
  • Won, Sung-Hye (Institute of Agricultural Science & Technology, Kyungpook National University) ;
  • Lee, Byung-Hyun (Division of Applide Life Science, Gyeongsang National University)
  • 발행 : 2004.03.01
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초록

이탈리안 라이그래스의 최적 조직배양 조건을 확립하기 위하여 성숙종자로부터 최적 배발생 캘러스 유도조건 및 효율적인 식물체 재분화 체계를 확립하였다. 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 5mg/L 2.4-D가 첨가된 MS배지에서 배발생 캘러스가 가장 높은 빈도로 유도되었다. 식물체 재분화는 배발생 캘러스를 1mg/L 2,4-D와 5mg/L BA가 첨가된 N6배지에서 배양했을 때 70%이상의 재분화율을 나타내었다. 기본배지의 종류에 따른 배양효율의 차이는 캘러스 유도에는 MS배지가, 식물체 재분화에는 N6배지가 효과적이었다. 배발생 캘러스의 형성율은 품종간에 큰 차이가 없었으나, 재분화 능력은 Jeanne 품종이 73%의 가장 높은 재분화율을 나타내어 품종간에 큰 차이를 나타내었다. 본 연구를 통하여 확립된 단기간 고효율 재분화 시스템은 분자육종을 통한 신품종 이탈리안 라이그래스의 개발에 유용하게 응용되어질 수 있을 것이다.

As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass (Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plant regeneration were evaluated. MS medium containing 5mg/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency was observed at above 70％ when embryogenic calli induced were transferred to N6 medium supplemented with 1mg/L 2,4-D and 5mg/L BA. Among several basic media tested, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. 'Jeanne' showed the highest regeneration frequency of 73％. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.

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