Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Micropropagation of an Endangered Species, Stellera rosea Nakai by Tissue Culture

멸종위기식물 피뿌리풀의 기내증식

  • Han, Mu-Seok (Warm-temperate Forest Research Center, Korea Forest Research Institute) ;
  • Moon, Heung-Kyu (Division of Biotechnology, Korea Forest Research Institute) ;
  • Kang, Young-Jae (Warm-temperate Forest Research Center, Korea Forest Research Inst) ;
  • Kim, Won-Woo (Warm-temperate Forest Research Center, Korea Forest Research Inst) ;
  • Kang, Byung-Seo (Warm-temperate Forest Research Center, Korea Forest Research Inst) ;
  • Byun, Kwang-Ok (Warm-temperate Forest Research Center, Korea Forest Research Inst)
  • 한무석 (국립산림과학원 난대산림연구소) ;
  • 문흥규 (국립산림과학원 산림유전자원부) ;
  • 강영제 (국립산림과학원 난대산림연구소) ;
  • 김원우 (국립산림과학원 난대산림연구소) ;
  • 강병서 (국립산림과학원 난대산림연구소) ;
  • 변광옥 (국립산림과학원 난대산림연구소)
  • Published : 2004.03.01
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Abstract

In order to develop an efficient micropropagation technique for an endangered species, Stellera rosea N., stem node cultures were conducted on MS medium supplemented with cytokinins. Generally, BA was better than zeatin on shoot proliferation from stem nodes, whereas zeatin showed more effective on shoot elongation. In vitro rooting of shoots was achieved by application of an auxin pre-culturing method. Overall rooting rate was relatively low and differed depending on the culture period. Pre-culturing of shoots for 15 days at 1.0mg/L IBA revealed a slightly better rooting efficiency reaching 30％ rooting rate than NAA. Root induction rate by NAA also varied with concentration of NAA and culture periods. Total 51％ of the rooted plantlets survived on artificial soil mixture and grew normally without any distinct morphological variation. The results suggest that the endangered Stetllera plants are propagated via in vitro culture system, but still need to more study for the improvement of rooting and acclimatization of the plantlets in soil.

멸종위기에 직면한 피뿌리풀 (Stellera roseaN.)의 기내증식법을 개발하고자 액아 마디를 MS 배지에 BAP와 zeatin을 처리하여 다경 (multiple shoot)를 유도하고 기내발근에 미치는IBA및 NAA처리 효과를 조사하였다. 액아 마디로부터 다경유도는 BA가 현저히 양호한 반면 줄기의 생장은 zeatin이 BA보다 효과적이었다. 증식된 줄기로부터 기내 발근은 오옥신 처리로 가능하였으나 발근율은 대체로 저조하였고 오옥신의 전처리 기간에 따라 차이를 보였다. IBA가 NAA보다 다소 좋은 발근효과를 보였고 1.0mg/L 농도로 15일간 배양시 30%까지 발근되었다. NAA역시 처리농도 및 처리기간에 따라 발근율에 차이를 보였다. 발근묘는 인공토양에서 51%가 활착되어 정상생장이 가능하였다. 이상의 결과는 발근 및 순화조건을 좀더 개선하면 피뿌리풀의 효율적인 기내번식이 가능함을 시사해준다.

Keywords

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