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The Korean Association of Immunobiologists (대한면역학회)
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B-1 Cells Differ from Conventional B (B-2) Cells: Difference in Proliferation

B세포의 증식에 있어 B-1 임파구와 B-2 임파구의 차이점에 대한 연구

  • Yeo, Seung Geun (Department of Otolaryngology, College of Medicine, Kyung Hee University) ;
  • Cho, Joong Saeng (Department of Otolaryngology, College of Medicine, Kyung Hee University) ;
  • Park, Dong Choon (Department of Obstetric and Gynecology, College of Medicine, Catholic University) ;
  • Rothstein, Thomas L. (Department of Medicine, School of Medicine and Immunobiology Unit, Evans Memorial Department of Clinical Research, Medical Center, Boston University)
  • 여승근 (경희대학교 의과대학 이비인후과학교실) ;
  • 조중생 (경희대학교 의과대학 이비인후과학교실) ;
  • 박동춘 (가톨릭대학교 의과대학 산부인과학교실) ;
  • Published : 2004.09.30
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Abstract

Background: B-1 cells differ from conventional B-2 cells both phenotypically and functionally. The aim of this study was to investigate the difference between peritoneal B-1 cells and splenic B-2 cells in proliferation. Methods: We obtained sorted B-1 cells from peritoneal fluid and B-2 cells from spleens of mice. During the culture of these cells, immunoglobulin secreted into the culture supernatants was evaluated by enzymelinked immunosorbent assay. Entering of S phase in response to LPS-stimuli was measured by proliferative assay. Results: Spontaneous Immunoglobulin M production occurred in peritoneal B-1 cells but not in splenic B-2 cells. LPS stimulated peritoneal B-1 cells secreted IgM at day 1, but splenic B-2 cells at day 2. In thymidine incorporation, peritoneal B-1 cells entered actively S phase after 24hours LPS-stimulation but splenic B-2 cells entered actively S phase after 48 hours. Conclusion: IgM secretion and S phase entering occurred early in peritoneal B-1 cells compared to splenic B-2 cells.

Keywords

References

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