Journal of Gastric Cancer

The Korean Gastric Cancer Association (대한위암학회)
권호 표지

Classification of Microsatellite Alterations Detected in Endoscopic Biopsy Specimens of Gastric Cancers

단순반복염기서열의 변이 형태에 따른 위암 내시경 조직의 유전자형 분류

  • Choi Young Deok (Departments of Microbiology, College of Medicine, The Catholic University of Korea) ;
  • Choi Sang Wook (Departments of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Jeon Eun Jeong (Departments of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Jeong Jeong Jo (Departments of Internal Medicine, College of Medicine, The Catholic University of Korea) ;
  • Min Ki Ouk (Departments of Microbiology, Pathology College of Medicine, The Catholic University of Korea) ;
  • Lee Kang Hoon (Departments of Microbiology, Radiology College of Medicine, The Catholic University of Korea) ;
  • Lee Sung (Departments of Microbiology, Surgery College of Medicine, The Catholic University of Korea) ;
  • Rhyu Mun Gan (Departments of Microbiology, College of Medicine, The Catholic University of Korea)
  • 최영덕 (가톨릭대학교 의과대학 미생물학교실) ;
  • 최상욱 (가톨릭대학교 의과대학 내과학교실) ;
  • 전은정 (가톨릭대학교 의과대학 내과학교실) ;
  • 정정조 (가톨릭대학교 의과대학 내과학교실) ;
  • 민기옥 (가톨릭대학교 의과대학 병리학교실) ;
  • 이강훈 (가톨릭대학교 의과대학 방사선과학교실) ;
  • 이성 (가톨릭대학교 의과대학 외과학교실) ;
  • 유문간 (가톨릭대학교 의과대학 미생물학교실)
  • Published : 2004.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Purpose: Individual gastric cancers demonstrate complicated genetic alterations. The PCR-based analysis of polymorphic microsatellite sequences on cancer-related chromosomes has been used to detect chromosomal loss and microsatellite instability. For the purpose of preoperative usage, we analyzed the correspondance rate of the microsatellite genotype between endoscopic biopsy and surgical specimens. Materials and Methods: Seventy-three pairs of biopsy and surgical specimens were examined for loss of heterozygosity and microsatellite instability by using 40 microsatellite markers on eight chromosomes. Microsatellite alterations in tumor DNAs were classified into a high-risk group (baselinelevel loss of heterozygosity: 1 chromosomal loss in diffuse type and high-level loss of heterozygosity: 4 or more chromosomal losses) and a low-risk group (microsatellite instability and low-level loss of heterozygosity: 2 or 3 chromosomal losses in diffuse type or $1\∼3$ chromosomal losses in intestinal type) based on the extent of chromosomal loss and microsatellite instability. Results: The chromosomal losses of the biopsy and the surgical specimens were found to be different in 21 of the 73 cases, 19 cases of which were categorized into a genotype group of similar extent. In 100 surgical specimens, the high-risk genotype group showed a high incidence of nodal involvement (19 of 23 cases: $\leq$5 cm; 23 of 24 cases: >5 cm) irrespective of tumor size while the incidence of nodal involvement for the low-risk genotype group depended on tumor size (5 of 26 cases: $\leq$5 cm; 18 of 27 cases: >5 cm). Extraserosal invasion was more frequent in large-sized tumor in both the high-risk genotype group ($\leq$5 cm: 12 of 23 cases; >5 cm: 23 of 24 cases) and the low-risk genotype group ($\leq$5 cm: 7 of 26 cases; >5 cm: 16 of 27 cases). The preoperative prediction of tumor invasion and nodal involvement based on tumor size and genotype corresponded closely to the pathologic tumor stage (ROC area >0.7). Conclusion: An endoscopic biopsy specimen of gastric cancer can be used to make a preoperative genetic diagnosis that accurately reflect the genotype of the corresponding surgical specimen.

Keywords