한국식품과학회지 (Korean Journal of Food Science and Technology)

한국식품과학회 (Korean Society of Food Science and Technology)
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국내산 주요 갈조류 추출물의 항혈액응고 활성

Anticoagulant Activities of Brown Seaweed Extracts in Korea

  • 발행 : 2004.12.31
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초록

국내산 주요 갈조류 18종의 추출용매 및 온도에 따른 항혈액응고 활성을 측정한 결과 미역, 다시마, 감태, 대황, 곰피, 톳, 괭생이 모자반의 열수 추출물의 APTT가 190.0초 이상으로 활성이 높았으며, 이 때의 추출온도는 $90^{\circ}C$였다. 감태의 에탄올 불용성분의 항혈액응고 활성은 $500\;{\mu}g/mL$에서 에탄을 가용성 물질에 비하여 2배 이상 증가하였으며, 에탄을 불용성분의 분자량에 따른 항혈액응고 활성은 $100\;{\mu}g/mL$ 농도에서 EKJ-eim 1(100 kDa 이상)이 190초 이상으로 EKJ-eim 2(100-50 kDa) 및 EKJ-eim 3(50-10 kDa)에 비해 좋았으며, 이 분회의 화학적 조성성분은 fucose, xylose, mannose, galactose, glucose, sulfate로 구성되어 있으며, 몰비는 1 : 0.05 : 0.10 : 0.15 : 0.17 : 1.46으로 2 mole의 fucose에 3 mole의 황산기가 결합되어 있는 산성다당임을 확인하였다.

Effects of extraction conditions and molecular fractionation on anticoagulant activities of major brown seaweeds in Korea were investigated. Hot water extracts of C. costata, U. pinnatifida (Sporophyte), L. japonica, K. crassifolia, E. stolonifera, E. bicyclis, S. horneri, and E. kurome increaced activated partial thromboplastin time (APTT) over 190 seconds, which may be related to intrinsic pathway of blood coagulation. Hot water extract of E. Kurome (EKJ) was further fractionated by ethanol precipitation. EKJ-eim, ethanol-insoluble material of EKJ, showed higher anticoagulant activity than EKJ. EKJ-eim was further fractioned with ultrafiltration. EKJ-eim 1, (over 100 kDa) fraction showed higher APTT activity than EKJ-eim. A EKJ-eim 1 was sulfated polysaccharide consisting of fucose, xylose, mannose, galactose, glucose and, sulfate at molar ratio of 1 : 0.05 : 0.10 : 0.15 : 0.17 : 1.46. The anticoagulant activity increased as sulfate content and molecular weight increased.

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