Applied Biological Chemistry

  • 제47권4호
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  • Pages.430-433
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  • 2004
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  • 2468-0834(pISSN)
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  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

유전자의 일시발현 분석용 숙주개발을 위한 카사블랑카백합(Lilium cv. Casablanca) 화분립의 이용

Utilization of Pollen Grain from Liluim cv. Casablanca as a Transient Gene Expression Host

  • 박희성 (대구가톨릭대학교 식물유전공학과)
  • Park, Hee-Sung (Department of Plant Biotechnology, Catholic University of Daegu)
  • 발행 : 2004.12.31
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초록

Lilium cv. Casablanca pollen grains stored at $-70^{\circ}C$ were grown in pollen germination medium with Agrobacterium tumefaciens LBA4404 cells harboring pBI121 for 18 hr at $27^{\circ}C$. Following this, cefotaxime (250 mg/L) was treated for 6 hr to eradicate the bacterial cells. Histochemical GUS analysis revealed that the transgenic pollen displayed deep blue color mostly from 12 hr after the co-cultivation. Presence of $200\;{\mu}M$ acetosyringone was determined not to be more effective for GUS transformation than its absence. GUS DNA integration in the transgenic pollen genomic DNA was clearly demonstrated by Southern blot analysis.

키워드

참고문헌

  1. Fisher, R., Vaquero-Martin, C., Sack, M., Drossard, J., Emans, N. and Commandeur U. (1999) Towards molecular farming in the future: transient protein expression in plants. Biotechnol. Appl. Biochem. 30, 113-116
  2. McCormick, S. (1993) Male gametophyte development. Plant Cell 5, 1265-1273 https://doi.org/10.1105/tpc.5.10.1265
  3. Taylor, L. P. and Helper, P. K. (1997) Pollen germination and tube growth. Annu. Rev. Plant Physiol. Plant Mol. Biol. 48, 461-491 https://doi.org/10.1146/annurev.arplant.48.1.461
  4. Aronen, T. S., Nikkanen, T. O. and Haggman, H. M. (1998) Compatability of different pollination techniques with microprojectile bombardment of Norway spruce and Scots pine pollen. Can. J. For. Res. 28, 79-86 https://doi.org/10.1139/cjfr-28-1-79
  5. Fernando, D. D., Owens, J. N. and Misra, S. (2000) Transientgene expression in pine pollen tubes following particle bombardment. Plant Cell Rep. 19, 224-228 https://doi.org/10.1007/s002990050003
  6. Hess, D., Dressler, K. and Nimmrichter, R. (1990) Transformation experiments by pipetting Agrobacterium into the spikelete of wheat (Triticum aestivum L.). Plant Sci. 72, 233-244 https://doi.org/10.1016/0168-9452(90)90087-5
  7. Tjokrokusumo, D., Heinrich, T., Wylie, S. Pottr, R, and McComb, J. (2000) Vacuum infiltration of Petunia hybrida with Agrobacterium tumefaciens to achieve plant transformation. Plant Cell Rep. 19, 792-797 https://doi.org/10.1007/s002990050009
  8. Luo, Z. X. and Wu, R. (1989) A simple method for the transformation of rice via the pollen-tube pathway. Plant Mol. Biol. Rep. 7, 69-77 https://doi.org/10.1007/BF02669248
  9. van der Leede-Plegt, L. M., Ven, B. C. E. K., Franken, J., van Tuyl, J. M., van Tunen, A. J. and Dons, H. J. M. (1997) Transgenic lilies via pollen-mediated transformation. Acta Hort. (ISHS) 430, 529-530
  10. Bethtold, N., Ellis, J. and Pelletier, G (1993) In planta Agrobaterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants, CR. Acad. Sci. Paris Life Sci. 316, 1194-1199
  11. Park, H. S. and Park, I. H. (2002). Analysis of UreB protein synthesis from transgenic lily pollen. Kor. J. Biotechnol. Bioeng. 17, 577-581
  12. Jefferson, R. A. (1987) Assaying chimeric genes in plants: the gus gene fusion system. Plant Mol. Biol. Rep. 5, 387-405 https://doi.org/10.1007/BF02667740
  13. Glick,B.R. and ompson, J.E.(1993)In Methods in Plant Molecular Biology and Biotechnology. CRC Press, Boca Raton, pp. 38-40
  14. Lohrke, S. M., Yang, H. and Jin, S. (2001) Reconstitution of acetosyhngone-mediated Agrobacterium tumefaciens virulencegene expression in the heterologous host Escherichia. coli. J. Bacteriol. 183, 3704-3771 https://doi.org/10.1128/JB.183.12.3704-3711.2001