Effects of Growth Regulators on Shoot Regeneration and Polysaccharide Production of Orostachys japonicus Berger

  • Kim, Won-Jung (Division of Forest Science, Gyeongsang National University) ;
  • Jung, Hee-Young (Division of Forest Science, Gyeongsang National University) ;
  • Min, Ji-Youn (Division of Forest Science, Gyeongsang National University) ;
  • Park, Dong-Jin (Division of Forest Science, Gyeongsang National University) ;
  • Kim, Yong-Duck (Division of Forest Science, Gyeongsang National University) ;
  • Kang, Young-Min (Division of Forest Science, Gyeongsang National University) ;
  • Choi, Myung-Suk (Institute of Agriculture & Life Sci., Gyeongsang Natl. Univ.)
  • Published : 2004.11.01

Abstract

Optimal culture conditions for efficient in vitro propagation and polysaccharide production of Orostachys japonicus were established. O. japonicus was cultured in media containing various growth regulators and carbon sources. The highest regeneration rate was achieved in 1.0 and $3.0\;mg\;l\;^{-1}$ of 2,4-D concentration, while the lowest was obtained in $10.0\;mg\;l\;^{-1}$ 2,4-D concentration. When different carbone sources were added in the culture medium, plant growth was high in 3% sucrose treatment. The micropropagated shoots were successfully acclimatized in artificial soils and produced comparable amont of polysaccharide compred to parent cultivated plants.

Keywords

References

  1. Ahn KS, Sim WS, Kim HM, Han SB, Kim IH (1996) Immunostimulating components from the root of Angelica gigas Nakai. Korean J. Pharmacogn. 27:254-261
  2. Ahn KS, Sim WS, Kim HM, Han SB, Kim IH (1998) Immunostimulating polysaccharide from cell culture of Angelica gigas Nakai. Biotechnology Letters 20(1):5-7 https://doi.org/10.1023/A:1005310609619
  3. Choi SD, Nam SH, Yang GJ, Cho MJ, Yang MS (1994) Plant regeneration from the stem tissue of Orostachys japonicus A. Berger. Korean J. Plant Tissue Culture 21(2):65-68
  4. Cunninghame ME, Hall JL (1985) A quantitative stereological analysis of the effect of indoleacetic acid on the dictyosomes in pea stem epidermal cells. Protoplasma 125: 230-234 https://doi.org/10.1007/BF01281242
  5. Dubois MC, Gilles KA, Hamilton JK, Rebers PA, Smith FRED (1956) Colorimetric method for determination of sugars and related sbstances. Analytical Chemistry 28(3):350-356 https://doi.org/10.1021/ac60111a017
  6. Eapen S, George L (1993) Somatic embryogenesis in peanut: Influence of growth regulators and sugars. Plant Cell Tiss. Org. Cult. 4: 151-156 https://doi.org/10.1007/BF00042273
  7. Francisco JP, Pablo M, Maritza B, Manuel P (2000) Effect of carbon source and sucrose concentration on growth and hexose accumulation of grape berries cultured in vitro. Plant Cell, Tissue and Organ Culture 61: 37-40 https://doi.org/10.1023/A:1006494918336
  8. Kim WJ, Kang YM, Park DJ, Huh GH, Lee BH, Choi MS (2004) Otimal culture conditions for In vitro propagation of Orostachys japonicus and enhancement of polysaccharide production. Korean J. Medicinal Crop Sci. 12(2) : 129-134
  9. Kojima Y, Kumazawa Y, Shibukawa N, Otsuka K, Mizunoe K (1980) 小鳥保彦 他. Proc. Symp. WAKAN-YAKU 13, 101
  10. Kumazawa Y, Mizunoe K, Otsuka Y (1982) Immunostimulating polysaccharides separated from hot water extract of Angelica acutiJoba Kitagawa (Yamato TohkD. Immunology 47,75-83
  11. Lazzeri PA, Hildebrand DF, Collins GB (1987) Soybean somatic embryogeneis: Effect of hormone and culture manipulations. plant Cell Tiss. Org. CuI. 190: 197-208
  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  13. Park HJ, Young HS, Kim JO, Rhee SH, Choi JS (1991) A study on the chemical constituents of Orostachys japonicus A. Berger. Korean J. Pharmacogn 22(2):78-84
  14. Proma K, Kofi EA, Richard FT (2002) Sugar profile of extracellular polysaccharides from different TremelJaspecies. International J. of Food Microbiology. 79:121-129 https://doi.org/10.1016/S0168-1605(02)00186-1
  15. Rao SR, GA Ravishankar (2002) Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol. Adv. 20: 101-153 https://doi.org/10.1016/S0734-9750(02)00007-1
  16. Quaite E, Parker RE, Steer MW (1983) Plant cell extension: structural implications for the origin of the plasma membrane. Plant Cell Environ 6: 429-432 https://doi.org/10.1111/j.1365-3040.1983.tb01277.x
  17. Shin DY, Lee YM, K HJ (1994) Anatomy and artificial seed propagation in anti-cancer plant Orostachys japonicus A. Berger. Korean J. Crop Sci. 39(2):146-157
  18. Taiz L, Zeiger E (1991) Plant Physiology. The Benjamin / Cummings Publishing Companym, Redwood City, CA
  19. Yamada H, Kawaguchi N, Ohmori T, Takeshita Y, Taneya S, Miyazaki T (1984a) Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides. Carbobydrate Res. 125,107-115. https://doi.org/10.1016/0008-6215(84)85146-0
  20. Yamada H, Kiyohara H, Cyong JC, Kojima Y, Kumazawa Y, Otsuka Y (1984b) Studies on polysaccharides from Angelica acutiloba. Part 1. Fractionation and biological properties of polysaccharides. Planta Med. 50, 163-167 https://doi.org/10.1055/s-2007-969661
  21. Yang MS, Choi SU (1992) Some biochemical component changes during the culture of Orostachys japonicus A. Berger. Korean J. Plant Tissue Culture 19(4):209-212
  22. Zhong JJ, Wang SJ (1998) Effects of nitrogen source on the production of ginseng saponin and polysaccharide by cell cultures of Panax quinquefolium. Process Biochemistry 33(6):671-675 https://doi.org/10.1016/S0032-9592(98)00030-2