생약학회지 (Korean Journal of Pharmacognosy)

한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

조록나무 Proanthocyanidin의 ${\alpha}-Amylase$ 및 ${\alpha}-Glucosidase$에 대한 저해 효과

Inhibitory Effects of Proanthocyanidin Extracted from Distylium racemosum on ${\alpha}-Amylase$ and ${\alpha}-Glucosidase$ Activities

  • 안진권 (국립산림과학원 생물공학과) ;
  • 박영기 (국립산림과학원 생물공학과) ;
  • 박소영 (국립산림과학원 생물공학과) ;
  • 김용무 (강원대학교 생명공학부) ;
  • 이해익 (강원대학교 생명공학부) ;
  • 이위영 (국립산림과학원 생물공학과)
  • Ahn, Jin-Kwon (Div. Biotechnology, Korea Forest Research Institute) ;
  • Park, Young-Ki (Div. Biotechnology, Korea Forest Research Institute) ;
  • Park, So-Young (Div. Biotechnology, Korea Forest Research Institute) ;
  • Kim, Yong-Mu (Division of Biotechnology, Kangwon National University) ;
  • Rhee, Hae-Ik (Division of Biotechnology, Kangwon National University) ;
  • Lee, Wi-Young (Div. Biotechnology, Korea Forest Research Institute)
  • 발행 : 2004.12.30
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초록

Distylium racemosum Sieb. Et Zucc contains some compounds inhibit -amylase activity in experimental conditions. The inhibitory test showed that 50% acetone extracts from the bark and leaves of the plant strongly inhibited salivary -amylase activity. Proanthocyanidin(PA) which has strong inhibitory activity was extracted from the leaves by chromatography on Sephadex LH-20. The inhibitory activities and the inhibition kinetics of the PA were studied against three kinds of enzymes: human salivary ${\alpha}-Amylase$ (SAA), pork pancreatin ${\alpha}-Amylase$ (PAA) and yeast ${\alpha}-Glucosidase$ (AG). Then the activities of PA against SAA, PAA and AG were compared with those of acarbose, a commercial agent. The inhibitory activities of PA were stronger than those of acarbose. Inhibition kinetics of the PA showed competitive inhibition for SAA and PAA, and non competitive inhibition for GA.

키워드

참고문헌

  1. Haslam, E. (1996) Natural polyphenols (vegetable tannins) as drugs: possible modes of action. J. Nat. Prod. 59: 205-215 https://doi.org/10.1021/np960040+
  2. Bernays, E. A, Cooper Driver, G., and Bilgener, M. (1989) Herbivores and plant tannins. In Begon, M., Fitter, AH., MacFadyen, A (Eds.), Advances in cological Research. vol. 19, 263-302. Academic Press, London https://doi.org/10.1016/S0065-2504(08)60160-9
  3. Aerts, R. J., Barry, T. N., and McNabb, W.C. (1999) Polyphenols and griculture: beneficial effects of proanthocyanidins in forages. Agric. Ecosys. Environ. 75: 1-12 https://doi.org/10.1016/S0167-8809(99)00062-6
  4. Yamagishi, M., Natsume, M., Osakabe, N., Nakamura, H., Furukawa, F., Imazawa, T., Nishikawa, A, and Hirose, M. (2002) Effects of cacao liquor proanthocyanidins on PhIPinduced mutagenesis in vitro, and in vivo mammary and pancreatic tumorigenesis in female Sprague-Dawley rats. Cancer Lett. 185: 123-130 https://doi.org/10.1016/S0304-3835(02)00276-8
  5. Bagchi, D., Sen, C. K., Ray, S. D., Das, D. K., Bagchi, M., Preuss, H. G., and Vinson, J. A (2003) Molecular mechanisms of cardioprotection by a novel grape seed proanthocyanidin extract. Mutat. Res. 523: 78-97
  6. Jagan Mohan Rao, L., Yada, H., Ono, H., Ohnishi-Kameyama, M., and Yoshida, M. (2004) Occurrence of antioxidant and radical scavenging proanthocyanidins from the Indian minor spice nagkesar (Mammea longifolia planch and triana syn). Bioorg. Med. Chem. 12: 31-36 https://doi.org/10.1016/j.bmc.2003.10.052
  7. Feng G. H., Richardson, M., Chen, M. S., Kramer, K. J., Morgan, T. D., and Reek, G. R. (1996) -Amylase inhibitors from wheat: Amino acid sequences and patterns of inhibition of insect and human $\alpha$-amylases. Insect Biochem. Molec. Biol. 26: 419-426 https://doi.org/10.1016/0965-1748(95)00087-9
  8. Markwick, N. P., Laing, W. A., Christeller, J. T., Reid, S. J., and Netwton, M. R. (1996) $\alpha$-amylase activities in larval midgut extraxcts from four species of Lepidoptera (Tortricidae and Gelechiidae): Response to pH and to inhibitors from wheat, barley, kidney bean, and streptomyces. J. Eeon. Entomol. 89: 39-45
  9. Rekha, M. R., Sasikiran, K., and Padmaja, G. (2003) Inhibitor potential of protease and $\alpha$amylase inhibitors of sweet potato and taro on the digestive enzymes of root crop storage pests. J. Stored Prod. Res. In press
  10. 문주석, 신창식, 최진상, 박석규, 심기환 (1995) 한국산 쌀보리 $\alpha$-amylase 저해물질의 분리 밑 정제. J. Korean Soc. Food Nutr. 24: 556-562
  11. Takahashi, T., Hiramoto, S., Wato, S., Nishimoto, T., Wada, Y., Nagai, K., and Yamaguchi, H. (1999) Identification of essential amino acid residues of an $\alpha$-amylase inhibitor from Phaseolus vulgaris white kidney beans. J. Biochem. 126: 838-844 https://doi.org/10.1093/oxfordjournals.jbchem.a022524
  12. Yamada, T., Hattori, K., and Ishimota, M. (2001) Purification and characterization of two $\alpha$-amylase inhibitors from seeds of tepary bean (Phaseolus acutifolius A. Gray). Phytochemistry 58: 59-66 https://doi.org/10.1016/S0031-9422(01)00178-9
  13. 전승호, 류일환, 박승택, 이갑상 (2001) White kidney bean (Phaseolus vulgaris)로부터 $\alpha$-amylase 저해제의 분리/정제. Korean J. Food Sci. Technol. 33: 117-121
  14. Miller, B. S. and Keen, E. (1947) The amylase inhibitor of Leoti sorghum. Archiv. Biochem. 15: 251-261
  15. 김제학, 김정우, 김하원, 심미자, 최응칠, 김병각 (1985) $\alpha$-amylase 저해제 생산 방선균의 선별과 분류 및 aamylase 저해제의 분리와 Kinetics 연구. Kor. J. Appl. Microbiol. Bioeng. 13: 223-232
  16. Lee, T. B. (1979) lllustrated flora of Korea. 424. Hyangmoonsa, Seoul
  17. Park, Y., Lee, W Y., Ahn, J. K., Lee, H. I., Chin, H. S., and Kwon, Y. J. (2003) Antioxidant compounds from Distylium racemosum leaves. J. Wood Sci. Technol. 31: 67-72 https://doi.org/10.1021/es960095y
  18. Booker, F. L., Anttonen, S., and Heagle, A. S. (1996) Catechin, proanthocyanidin and lignin contents of loblolly pine (Pinus taeda) needles after chronic exposure to ozone. New Phytol. 132: 483-492 https://doi.org/10.1111/j.1469-8137.1996.tb01868.x
  19. Bohm, B. A (1998) IntroductIon to flavonoids. In Ravindranath, B. (Eds.), Chemistry and biochemistry of organic natural products. 503. Hardwood academic publishers, India.
  20. Yoon, S. H. and Robyt, Y. J. (2003) Study of the inhibition of four alpha amylases by acarbose and its $4^{IV}-\alpha-$maltohexaosyl and $4^{IV}-\alpha-$maltododecaosyl analogues. Carbohydrate Res. 338: 1969-1980 https://doi.org/10.1016/S0008-6215(03)00293-3
  21. Blanco, L. A and Iturbe, C. F. A (1981) Purification and characterization of an $\alpha-$amylase inhibitor from maize(Zeamays). J. Food Biochem. 5: 1-14 https://doi.org/10.1111/j.1745-4514.1981.tb00658.x
  22. Mitsunaga, T., Abe, L., Kontani, M., Ono, H., and Tanaka, T. (1997) Inhibitory effects of bark proanthocyanidins on the activities of glucosyltransferases of Streptococcus sobrinus. J. Wood Chem. Technol. 17: 327-340 https://doi.org/10.1080/02773819708003134