생약학회지 (Korean Journal of Pharmacognosy)

한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

시험관내에서 홍화의 물 추출물이 T 및 B 림프구의 활성에 미치는 영향

Effect of Water Extract of Carthamus tinctorious L. on In Vitro Activity of T and B Lymphocytes

  • 최윤화 (전주대학교 과학기술학부 생명과학전공) ;
  • 도정수 (전주대학교 과학기술학부 생명과학전공) ;
  • 남상윤 (전주대학교 과학기술학부 생명과학전공)
  • Choi, Youn-Hwa (Department of Biological Science, School of Science and Technology, Jeonju University) ;
  • Do, Jeong-Su (Department of Biological Science, School of Science and Technology, Jeonju University) ;
  • Nam, Sang-Yun (Department of Biological Science, School of Science and Technology, Jeonju University)
  • 발행 : 2004.12.30
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초록

Based on the traditional application of Carthamus tinctorious L. (CF) as a component of Korean medicinal decoctions, in the present study, we investigated in vitro an immunomodulatory activity of water extract of CF(WECF). Water extract of CF significantly increased the in vitro proliferative responses of spleen cells (SPC). However, addition of WECF during anti-CD3 activation resulted in a significant decrease in SPC proliferation. Flow cytometric analysis showed that WECF addition chanced T and B cell frequencies in anti-CD3-activated spleen cell populations. Using purified cells, it was revealed that WECF is mitogenic to B cells but rather inhibitory to T cell Proliferation. Upon anti-CD3 stimulation, high concentration (1 mg/ml) of WECF significantly inhibited T cell proliferation until day 2 of stimulation. At day 3, anti-CD3-activated cells exposed to WECF recovered their proliferation to the level comparable to control. Although B cell proliferation was also inhibited in proliferation at day 1, it recovered sooner and then was rather augmented by WECF at day 3. These data indicate that WECF down-regulates lymphocyte proliferation at early phase of activation but T cells are more vulnerable than B cells to WECF, However, CD4+ and CD8+ T cells did not differ in WECF-mediated immunotoxicity. Data of propidium iodide (PI) staining showed that WECF accelerates activated T cell, but not B cell, apoptosis and WECF concurrently inhibited cytokine production of activated T cells. Taken together, WECF exhibits B cell mitogenic activity and differential toxicity more pronounced to T cells, suggesting a possible in vivo application of WECF for specific control of T cells without alteration of B cell activity.

키워드

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