생약학회지 (Korean Journal of Pharmacognosy)

한국생약학회 (The Korean Society of Pharmacognosy)
권호 표지

삼릉, 황금, 살송편버섯 혼합추출물의 항종양 작용

Antitumor Effects of SKT (Skullcap - Knope sedge - Trametes) Mixture Extract

  • 신숙정 (전북대학교 의과대학 미생물학교실) ;
  • 이정호 (전북대학교 의과대학 미생물학교실)
  • Shin, Sook-Jeong (Department of Microbiology, Chonbuk National University Medical School) ;
  • Lee, Jeong-Ho (Department of Microbiology, Chonbuk National University Medical School)
  • 발행 : 2004.12.30
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초록

SKT is consisted of skullcap radix, knope-sedge radix and trametes mushroom. SKT mixture extract has been used for curing breast cancer and cervical cancer as a folk medicine without any kind of experimental evidence to support the rationales for its clinical use. This study was undertaken to investigate the antitumor effects and toxicity of SKT. Tumor was induced by implantation of B16F10 melanoma cells $(1{\times}10^6\;cells/mouse)$ into abdominal skin in ICR mice and SKT application (5 mg/mouse, p.o.) was initiated 4 days prior to tumor induction and lasted for 42 days. SKT significantly inhibited not only tumor growth but also metastasis of i.v. implanted melanoma cells into lung and showed prolonged life span of tumor bearing mice. The combined theraphy of SKT with doxorubicin was more effective against tumor metastasis into lung. SKT almostly recovered serum SGPT to normal level of galactosamine/LPS-induced hepatitis mice. High dose of SKT did not show any acute side effects. But, in vitro SKT did not inhibit the growth of melanoma cells, which suggests that the antitumor effects of SKT might be menifested by indirect mechanisms.

키워드

참고문헌

  1. Schmidt, J. D., Gibbons, R. P., Johnson, D.E., Prout, G. R., Scott, W. W. and Murphy, G. P. (1976) Chemotherapy of advanced prostatic cancer. Evaluation of response parame-ters. Urology. 7: 602-610 https://doi.org/10.1016/0090-4295(76)90085-6
  2. Roland, J. F., Chmielewicz, Z. F., Weiner, B. A, Gross, A. M., Boening, O. P., Luck, J. V., Bardos, T. J., Really, H. C., Sugiura, K, Stock, C. C., Lucas, E. H., Byerrum, R U., and Stevens, J. A (1987) Calvacine, a new antitumor agent. Science. 132-141
  3. Ortaldo, J. R and Longo, D.L. (1988) Human natural lymphocyte effector cell, Definition, analysis of a activity and effectiveness. J Nati. Can. Inst. 80: 999-1008 https://doi.org/10.1093/jnci/80.13.999
  4. Nielsin, H. J., Moesgaard, F., and Kehlet, H. (1989) Ran-itidine for prevention of postoperative suppression of delay-ted hypersensitivity. Am. J. Surg. 157: 291-294 https://doi.org/10.1016/0002-9610(89)90553-9
  5. Franz G. (1989) Polysaccharides in pharmacy; current appli-cation and future concepts. Planta. Med. 55: 493-497 https://doi.org/10.1055/s-2006-962078
  6. Ikekawa, T., Uehara, N., Maeda, Y., Nakanishi, M., and Fukuoka, F. (1969) Antitumor activity of aqueous extract of edible mushrooms. Cancer. Res. 9: 734-736
  7. Hamuro, J., Maeda, Y. Y., Arai, Y., Fukuoka, F., and Chihara, G. (1971) The significance of the higher structure of the polysaccha rides lentinan and pachymaran with regard to their antitumour activity. Chem. BioI. Interact. 3: 69-71 https://doi.org/10.1016/0009-2797(71)90026-3
  8. Hirata. N., Tsuzuki, A, Ohno, N., Saita, M., Adachi, Y., and Yadomae, T. (1998) Cytokine synthesis of human monocytes stimulated by triple or single helical conformer of an antitumour (3)-beta-D-glucan preparation, sonifilan. Zentralbl. Bakterio. 288: 403-413 https://doi.org/10.1016/S0934-8840(98)80015-7
  9. Kiyohara. H., Matsumoto, T., and Yamada, H. (2004) Combination effects of herbs in multi-herbal formula: Expression of juzen-tai-ho-to's immuno-modulatory activity on the intestinal immun sys- system. Evid Based Complement Alternat Med. 1(1): 83-91 https://doi.org/10.1093/ecam/neh004
  10. Yano, H., Mizoguchi, A., Fukuda, K., Haramaki, M., Ogasawara, S., Momosaki, S., and Kojiro, M. (1994) The herbal medicine sho-sai- ko-to inhibits proliferation of cancer cell lines by inducing apoptosis and arrest at the GO/G1 phase. Cancer. Res. 15:54(2): 118-154
  11. 신숙정, 이정호, 문용(1989) 황금 추출물이 면역기능, 미생물증식 및 세균변이에 미치는 영향. 대한면역학회지, 20: 343-348
  12. Razina, T. G., Udintsev, S.N., Tiutrin, l.l., Borovskaia, TG., and Iaremenko, K.V. (1989) The role of thrombocyte aggregation funtion in the mechanism of the antimetastatic action of an etract of Baikal skullcap. Vopr. Onkol. 35: 331-342
  13. Hansen, M.B., Nielsen, S. E., and Berg, K. (1989) Reex-amination and futher development of a precise and rapid dye method for measuring cell growth/cell kill. J. Immunol. Methods. 119: 203-210 https://doi.org/10.1016/0022-1759(89)90397-9
  14. Moorikawa, K., Takeda, R., and Yamazaki, M. (1985) Induction of tumoricidal activity of polymorphonuclear leukocytes by a linear beta-1,3-D-blucan and other immunomodulators in murine cells. Cancer. Res. 45: 1496-1501
  15. 이정호, 김학군, 하대유(1993) 화살나무의 항종양 작용과 그 기전. 대한면역학회지, 15: 243-253
  16. Ohno, T., Inoue, M., and Ogihara, Y. (2002) Suppressive effect of hichimo tsukokato(Kampo medicine) on pulmonary metastasis of B16 melanoma cells. BioI. Pharm. Bull. 25: 880-884 https://doi.org/10.1248/bpb.25.880
  17. Tiegs, G., Wolter, M., and Wendel, A. (1989) Tumor necrosis factor is terminal mediator in galactosamin/endotoxininduced hepatitis in mice. Biochem. Pharmacol. 38: 627-631 https://doi.org/10.1016/0006-2952(89)90208-6
  18. Tan, C., Etcubanas, E., Wollner, N., Rosen, G., Gilladoga, A, Showel, J., Murphy, M.L., and Krakoff, l.H. (1973) Adriamycin, an antitumor antibiotic in the treatment of neoplastic disease. Cancer. 32: 9-17 https://doi.org/10.1002/1097-0142(197307)32:1<9::AID-CNCR2820320102>3.0.CO;2-6
  19. Maeda, N. (2004) Kampo medicine and new immunological parameter. Rinsho. Byori. (Article in japanese) 52(5): 448-451
  20. Ikegami, F., Fujii, Y., ishihara, K., and Satoh, T. (2003) Toxicological aspects of Kampo medicines in clinical use. Chem. BioI. IN-teract. 15(3): 235-240
  21. Zee-Cheng, R.K. (1992) Shi-quan-da-bu-tang(ten significant tonic decoction),SQT. A potent Chinese biological response modifier in cancer immunotherapy, potentiation and detoxification of anticancer drugs. Methods Find. Exp. Clin. Pharmacol. 14(9): 725-736