Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Downregulatory Effect of AGI-1120 $({\alpha}-Glucosidase Inhibitor)$ and Chaga Mushroom (Inonotus obliquus) on Cellular $NF-{\kappa}B$ Activation and Their Antioxidant Activity

AGI-1120과 차가버섯의 $NF-{\kappa}B$ 활성화 억제 및 항산화 효과

  • Song, Hee-Sun (Department of Clinical Pathology and Food & Nutrition, and Bioindustry & Technology Research Institute, Kwangju Health College) ;
  • Lee, Young-Jong (Department of Clinical Pathology and Food & Nutrition, and Bioindustry & Technology Research Institute, Kwangju Health College) ;
  • Kim, Seung-Kyoon (Department of Clinical Pathology and Food & Nutrition, and Bioindustry & Technology Research Institute, Kwangju Health College) ;
  • Moon, Won-Kuk (En-Biotechnology Co., Ltd) ;
  • Kim, Dong-Woo (En-Biotechnology Co., Ltd) ;
  • Kim, Yeong-Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Moon, Ki-Young (Department of Clinical Pathology and Food & Nutrition, and Bioindustry & Technology Research Institute, Kwangju Health College)
  • 송희순 (광주보건대학 임상병리과, 식품영양과, 생명산업기술연구소) ;
  • 이영종 (광주보건대학 임상병리과, 식품영양과, 생명산업기술연구소) ;
  • 김승균 (광주보건대학 임상병리과, 식품영양과, 생명산업기술연구소) ;
  • 문원국 (엔바이오테크놀러지 연구소) ;
  • 김동우 (엔바이오테크놀러지 연구소) ;
  • 김영식 (서울대학교 약학대학 천연물과학연구소) ;
  • 문기영 (광주보건대학 임상병리과, 식품영양과, 생명산업기술연구소)
  • Published : 2004.03.30
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Abstract

Effect of AGI $({\alpha}-Glucosidase\;Inhibitor)-1120$, pine (Pinus densiflora) bark extract and Chaga mushroom (Inonotus obliquus) - and Chaga mushroom mycelium extracts on cellular $NF-{\kappa}B$ activation in malignant human keratinocytes (SCC-13) were evaluated to elucidate the possible correlation of $NF-{\kappa}B$ with antioxidant activity. The antioxidant activities of these natural products were examined in three different evaluation methods, i.e., lipid peroxidation value (POV) evaluation test, and 1,1diphenyl-2-picrylhydrazyl radical (DPPH) and nitric oxide (NO) scavenging test. In a cell-based $NF-{\kappa}B$ monitoring assay systern, all samples revealed the downregulatory profiles on the cellular $NF-{\kappa}B$ activity. AGI -1120 (1, 2 mg) and Chaga mushroom extract (0.05, 0.1 mg) downregulated the $NF-{\kappa}B$ activity in a dose-dependent manner. Chaga mushroom mycelium extract (5 mg) significantly inhibited the $NF-{\kappa}B$ activity (p<0.05). Although AGI-1120 and Chaga mushroom mycelium extract exhibited no antioxidant activities evaluated in pay, Chaga mushroom extract showed antioxidant in a dose-dependent manner at concentrations of $0.05{\sim}1$ mg. While AGI-1120 and Chaga mushroom extract possessed a relatively potential DPPH radical scavenging activity, the NO scavenging activity of Chaga mushroom extract $(SC_{50}:47\;{mu}g)$ was higher than the known antioxidant, vitamin C $(SC_{50}:77\;{mu}g)$. These results suggest that AGI-1120 and Chaga mushroom- and Chaga mushroom mycelium extracts may serve as an useful radical scavenging antioxidant agents with $NF-{\kappa}B$ inhibitory effect in human skin.

Keywords

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