Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Antioxidant Activity and Total Phenolic Content of Triphala churna

  • Jayajothi, E. (Department of Pharmacognosy and Pharmaceutical Chemistry, C. L. Baid Metha College of Pharmacy) ;
  • Elavarasu, T. (Department of Pharmacognosy and Pharmaceutical Chemistry, C. L. Baid Metha College of Pharmacy) ;
  • Hamsaveni, M. (Department of Pharmacognosy and Pharmaceutical Chemistry, C. L. Baid Metha College of Pharmacy) ;
  • Sridhar, S.K. (Department of Pharmacognosy and Pharmaceutical Chemistry, C. L. Baid Metha College of Pharmacy)
  • Published : 2004.02.28
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Abstract

Triphala churna is a widely used herbal formulation that contains equal proportion of dried fruit powder of Emblica officinalis, Terminalia chebula and Terminalia belirica. In the Indian system of medicine, it is used in cleaning wounds, urinary disorders, diabetes mellitus, leprosy, constipation, eyesight promotion, piles, and as a rejuvenator. In the present study, the methanolic extract of 5 commercial Triphala was evaluated for antioxidant activity by 1,1-diphenyl-2-picryl-hydrazyl free radical scavenging method, total phenolic content by Folin-Ciocalteu method and gallic acid equivalents (GAE) by high performance thin layer chromatographic (HPTLC) method. All extracts exhibited antioxidant activity significantly. The $IC_{50}$ of the extracts ranged between $7.16\;to\;12.96\;{\mu}g/ml$. The total phenolic content of the extracts was found to be 195.3-296.4 mg of GAE/gm of GAE/gm dw. The HPTLC chromatographic data reveal that the content of GAE present in the extract was found to be $7.17-4.11\;{\mu}g/ml$. The study reveals that out of the churnas analysed, C was found to exhibit the most potent antioxidant activity. A clear correlation between $IC_{50}$ and content of GAE nor the total phenolic content could be observed. The study reveals that the consumption of Triphala would exert several beneficial effects by virtue of its antioxidant activity.

Keywords

References

  1. Ahn, M. J., Kim, C. Y, Lee, J. S., Kim, T. G., Kim, S. H., Lee, C. HIV-l integrase by galloyl glucoses from Tenninalia chebula and flavonol glycoside gallates from Euphorbia pekinensis. Ptanta Med. 68, 457-459 (2002) https://doi.org/10.1055/s-2002-32070
  2. Ajitha, M. and Rajnarayana, K., Role of free radicals in human diseases, Indian Drugs. 38, 545-555 (2001)
  3. Anila, L. and Vijayalakshmi, N. R., Beneficial effects of flavonoids from Sesamum indicum, Emblica officinalis and Momordica charantia. Phytother. Res. 14, 592-595 (2000) https://doi.org/10.1002/1099-1573(200012)14:8<592::AID-PTR772>3.0.CO;2-#
  4. Anila, L. and Vijayalakshmi, N. R., Flavonoids from Emblica officnalis and Mangifera indica-effectivensss for dyslipidemia. J. Ethnopharmacol. 79, 81-87 (2002) https://doi.org/10.1016/S0378-8741(01)00361-0
  5. Asmawi, M. Z., Kankaanranta, H., Moilanen, E. and Vapaatalo, H., Antiinflammatory activides of Embtica officinalis Gaertn leaf extracts. J. Pharm. Pharmacol. 45, 581-584 (1993) https://doi.org/10.1111/j.2042-7158.1993.tb05605.x
  6. Bhattacharya, S. K., Bhattacharya, A., Sairam, K. and Ghosal, S., Effect of bioactive tannoid principles of Emblica officinalis on ischemia-reperfusion-induced oxidative stress in rat heart. Phytomedicine. 9, 171-174 (2002) https://doi.org/10.1078/0944-7113-00090
  7. Bhattacharya, A., Kumar, M., Ghosal, S., Bhattacharya, S. K. 2000. Effect of bioactive tannoid principles of Emblica officinalis on iron-induced hepatic toxicity in rats. Phytomedicine. 7, 173-175 https://doi.org/10.1016/S0944-7113(00)80091-4
  8. Biswas, S., Talukder, G. and Sharma, A., Protection against cytotoxic effects of arsenic by dietary supplementation with crude extract of Emblica officinalis fruit. Phytother. Res. 13, 513-516 (1999) https://doi.org/10.1002/(SICI)1099-1573(199909)13:6<513::AID-PTR525>3.0.CO;2-6
  9. Chaudhuri, R. K., Emblica cascading antioxidant: a novel natural skin care ingredient. Skin Pharmacol. Appl. Skin Physiol. 15, 374-380 (2002) https://doi.org/10.1159/000064544
  10. Dash, V. B. and KashyaP, V.L., Materia Medica of Ayurveda. Concept Publishing Company, New Delhi, India, 1987
  11. Droge, W., Agingrelated changes in the thiol/disulfide redox state: implications for the use of thiol antioxidants. Exp. Gerontol. 37, 1333-1345 (2002) https://doi.org/10.1016/S0531-5565(02)00175-4
  12. Ferrari, C. K. and Torres, E. A., Biochemical pharmacology of functional foods and prevention of chronic diseases of aging. Biomed Pharmacother. 57, 251-260 (2003) https://doi.org/10.1016/S0753-3322(03)00032-5
  13. Freund, J. E. and Walpore, R. E., Mathematical Statistics. Prentice-Hall Inc, New Jersey, 1980
  14. Haque, R., Bin Hafeez, B., Ahmad, I., Parvez, S., Pandey, S. and Raisuddin, S., Protective effects of Emblica officinalis Gaertn. in cyclophosphamide-treated mice. Hum. Exp. Toxicol. 20, 643-650 (2001) https://doi.org/10.1191/096032701718890568
  15. Hamada, S., Kataoka, T, Woo, J. T, Yamada, A., Yoshida, T., Nishimura, T., Otake, N. and Nagai, K., Immunosuppressive effects of gallic acid and chebulagic acid on CTL-mediated cytotoxicity. Biol. Pharm. Bull. 20, 1017-1019 (1997) https://doi.org/10.1248/bpb.20.1017
  16. Hatano, T., Edmatsu, R., Hiramatsu, M., Mori, A., Fujita, Y., Yasuhara, A., Yoshida, T. and Okuda, T., Effects of the interaction of tannins and related polyphenols on superoxide anion radical and on 1,1-dipheny1-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37, 2016-2021 (1989) https://doi.org/10.1248/cpb.37.2016
  17. Kar, A., Choudhary, B.K. and Bandyopadhyay, N.G., Comparative evaluation of hypoglycaemic activity of some Indian medicinalplants in alloxan diabetic rats. J. Ethnopharmacol. 84, 105-108 (2003) https://doi.org/10.1016/S0378-8741(02)00144-7
  18. Kaur, S., Arora, S., Kaur, K. and Kumar, S., The in vitro antimutagenic activity of Thphalaan Indian herbal dmg. Food Chem. Toxicol. 40, 527-534 (2002) https://doi.org/10.1016/S0278-6915(01)00101-6
  19. Khan, M. T., Lampronti, L., Martello, D., Bianchi, N., Jabbar, S., Choudari, M. S., Datta, B. K. and Gambari, R., Identification of pyrogallol as an antiproliferative compound present in extracts from the medicinal plant Emblica officinatis;. effects on in vitro cell growth of human tumor cell lines. Int. J. Oncol. 21, 187-192 (2002)
  20. Kumazawa, S., Taniguchi, M., Suzuki, Y, Shimura, M., Sunkwon, M. and Nakayama, T., Antioxidant activity of polyphenols in carob pods. J. Agric. Food Chem. 50, 373-377 (2001)
  21. Kusumoto, I. T., Kakiuchi, N., Hatton, M., Namba, T., Sutaradjo, S. and Shimotohnok, K., Screening of some Indonesian medicinal plants for inhibitory effect on HIV-1 protease. Shoyakugaku. Zasshi. 46, 190-193 (1992)
  22. Moeslinger, T., Fiiedl, R., Volf, I., Brunner, M., Koller, E. and Spieckermann, P. G., Inhibition of inducible nitdc oxide synthesis by the herbal preparation Padma 28 in macrophage cell line. Can. J. Physiol. Pharmacol. 78, 861-866 (2000) https://doi.org/10.1139/cjpp-78-11-861
  23. Sabu, M. C. and Kuttan, R., Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property. J. Ethnopharmacol. 81, 155-160 (2002) https://doi.org/10.1016/S0378-8741(02)00034-X
  24. Sairam, M., Neetu, D., Yogesh, B., Anju, B., Dipti, R, Pauline, T., Sharma, S.K., Sarada, S.K., Ilavazhagan, G., Kumar, D. and Selvamurthy, W., Cyto-protective and immuno-modulating properties of Amla (Embtica ojficinaUs) on lymphocytes: an in-vitro study. J. Ethwpharmacol. 81, 5-10 (2002) https://doi.org/10.1016/S0378-8741(01)00421-4
  25. Saleem, A., Ahotupa, M. and Pihlaja, K., Total phenolics concentration and aiitioxidant potential of extracts of medicinal plants of Pakistan. Z. Naturforsch[C]. 56, 973-978 (2001)
  26. Saleem, A., Husheem, M., Harkonen, P. and Pihlaja, K., Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit. J. Ethnophamacol. 81, 327-336 (2002) https://doi.org/10.1016/S0378-8741(02)00099-5
  27. Shaila, H. P., Udupa, S. L. and Udupa, A. L., Hypolipidemic activity of three indigenous drugs in experimentally induced atherosclerosis. Int. J. Cardiol. 67, 119-124 (1998) https://doi.org/10.1016/S0167-5273(98)00281-2
  28. Shin, T. Y, Jeong, H. J., Kim, D. K., Kim, S. H., Lee, J. K., Kim, D. K., Chae, B. S., Kim, J. H., Kang, H. W., Lee, C. M., Lee, K. C., Park, S. T., Lee, E. J., Lim, J. R, Kim, H. M. and Lee, Y. M., Inhibitory action of water soluble fraction of Terminalia chebula on systemic and local anaphylaxis. J. Ethnopharmacol. 74, 133-140 (2001) https://doi.org/10.1016/S0378-8741(00)00360-3
  29. Sivarajan, V. V. and Balachandran, I., Ayurvedic Drugs and their Plant Sources. Oxford and IBH Publishing Company, New Delhi, India, 1994
  30. Suguna, L., Singh, S., Sivakumar, R, Sampath, P. and Chandrakasan, G., Influence of Terminalia chebula on dermal wound healing in rats. Phytother. Res. 16, 227-231 (2002) https://doi.org/10.1002/ptr.827
  31. Thorat, S. R, Rege, N. N., Naik, A. S., Thatte, U. M., Joshi, A., Panikar, K. N., Bapat, R. D. and Dahanukar, S. A., Emblica officinatis: a novel thempy for acute pancreatitis-an experimental study. HPB. Surg. 9, 25-30 (1995) https://doi.org/10.1155/1995/51310