The Effects of Chungganhaeju-tang(Qingganjiejiu-tang) on $NF{\kappa}B$ Activation and Apoptosis of Kupffer Cells

청간해주탕(淸肝解酒湯)이 kupffer cell의 $NF{\kappa}B$ 활성화 및 세포사멸에 미치는 영향

  • Han Chang-Woo (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Kim Young-Chul (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Woo Hong-Jung (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University) ;
  • Lee Jang-Hoon (Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University)
  • 한창우 (경희대학교 한의과대학 간계내과학교실) ;
  • 김영철 (경희대학교 한의과대학 간계내과학교실) ;
  • 우홍정 (경희대학교 한의과대학 간계내과학교실) ;
  • 이장훈 (경희대학교 한의과대학 간계내과학교실)
  • Published : 2004.12.01

Abstract

Objectives : Previous studies showed that treatment with Chungganhaeju-tang prevents hepatic inflammation and apoptosis in alcoholic liver disease. The purpose of our study is to determine if any relations exsists between the transcription factor $NF{\kappa}B$, an orchestrating expression of a large number of genes and inhibitory effects of Chungganhaeju-tang on ethanol induced apoptosis. Materials and Methods : To assess the role of $NF{\kappa}B$, we blocked NFkB activation by delivering to the kupffer cells $I{\kappa}B{\Delta}N$, a dominant negative $NF{\kappa}B$ inhibitor, and investigated if Chungganhaeju-tang still prevented apoptosis. Results : When $NF{\kappa}B$ activation was blocked, there was no inhibitory effect of Chungganhaeju-tang on ethanol induced apoptosis of kupffer cells. Conclusion : This result suggests that Chungganhaeju-tang protects the liver from ethanol induced apoptosis by activating the $NF{\kappa}B$ that plays a key role in porotecting mechanism and reducing inflammatory cytokine gene expression.

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