Korean Journal of Plant Taxonomy (식물분류학회지)

The Korean Society of Plant Taxonomists (한국식물분류학회)
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Phylogeny of Scopolia Jacq. s. str. based on ITS sequences

ITS 염기서열에 의한 미치광이풀속의 계통

  • Received : 2003.09.26
  • Accepted : 2003.11.08
  • Published : 2003.12.31
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Abstract

Internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA were determined for 14 individuals representing eight taxa from Scopolia s. str. and related genera, Anisodus and Atropanthe. We found that the ITS sequences of Korean endemic species, S. parviflora, are significantly different from its allied species, S. japonica. This is contradictory to traditional taxonomic treatments in which those species are regarded as conspecific. S. parviflora exhibited closer relationship to S. carniolica, which is disjunctly distributed in Europe. In spite of substantially high sequence divergence between S. japonica and S. parvlflora/S. carniolica clade, morphological resemblance is evident among the species. Morphological stasis concept (retardation of morphological differentiation or evolution of similar characters among the disjuncts in a similar ecological habitat) was referred to understand this rather unusual evolutionary feature. S. lutescens, another Korean endemic species, shared almost identical ITS sequences with S. parviflora. Lack of diagnostic character distinguishing the taxa suggests that they are conspecific. Anisodus carniolicoides, which was originally described in Scopolia, was grouped with A. luridus and A. tanguticus. The monophyletic Anisodus formed a sister group relationship with a monotypic genus Atropanthe.

Scopolia s. str.(미치광이풀속) 및 그 근연속인 Anisodus, Atropanthe 등을 대표하는 8 분류군으로부터 얻은 14개체를 대상으로 핵 ribosome DNA의 ITS 염기서열 결정을 실시하였다. 그 결과 한반도 고유종인 S. parviflora(미치광이풀)은 ITS 전체 길이와 염기서열 변이에 있어서 근연종인 S. japonica와 매우 큰 차이가 있음이 밝혀졌다. 이와 같은 결과는 두 종을 동일시했던 대부분의 분류학적 견해와 배치되는 것이다. 한편, S. parviflora는 유럽에 격리분포하는 종인 S. carniolica와 더 가까운 계통유연관계를 나타냈다. S. japonica가 S. parviflora 및 S. carniolica와 높은 유전적 차이를 나타내는 것과는 대조적으로 매우 높은 형태적 유사성을 보이는 것은 이들이 오랜 격리기간에도 불구하고 유사한 생육환경에 처함으로 인해 형태진화가 지연되었거나 비슷한 형질을 갖는 방향으로 진화가 일어났을 것이라는 형태정체(morphological stasis) 개념으로 이해되었다. 다른 한반도 고유종인 S. lutescens(노랑미치광이풀)은 S. parviflora와 ITS 염기서열이 거의 동일하였고 계통수 상에서도 두 종에 속하는 개체들이 전혀 구분되지 않는 것으로 밝혀졌다. 자연개체군 내에서 이들 두 종을 구분하는 주요 식별형질들의 유용성도 결여되어 S. lutescens는 S. parviflora의 품종 혹은 단순한 개체 변이로 이해되었다. 한편, Scopolia속으로 최초 기재되었다가 화분의 형질에 의해 Anisodus속으로 이전되었던 A. carniolicoides는 A. luridus 및 A. tanguticus와 단계통군을 형성하였으며 Anisodus는 단형속인 Atropanthe와 자매군 관계를 형성하였다.

Keywords

Acknowledgement

Supported by : 한림대학교

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