Clinical and Experimental Pediatrics
- 제46권8호
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- Pages.789-794
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- 2003
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- 2713-4148(eISSN)
저산소-허혈 손상이 신생 흰쥐의 뇌 선조체(Striatum) Monoamine 대사에 미치는 영향
Effect of Hypoxia-Ischemia on Striatal Monoamine Metabolism in Neonatal Rat Brains
- 지윤희 (단국대학교 의과대학 소아과학교실) ;
- 김형건 (단국대학교 의과대학 약리학교실) ;
- 박우성 (단국대학교 의과대학 소아과학교실) ;
- 장영표 (단국대학교 의과대학 소아과학교실)
- Jee, Youn Hee (Department of Pediatrics, Collage of Medicine, Dankook University) ;
- Kim, Hyung Gun (Department of Pharmacology, Collage of Medicine, Dankook University) ;
- Park, Woo Sung (Department of Pediatrics, Collage of Medicine, Dankook University) ;
- Chang, Young Pyo (Department of Pediatrics, Collage of Medicine, Dankook University)
- 투고 : 2003.03.21
- 심사 : 2003.05.10
- 발행 : 2003.08.15
초록
목 적: 신생 흰쥐의 뇌에 저산소-허혈을 유발하여 선조체 monoamine과 그 대사물들의 변화를 관찰하여, 저산소-허혈 손상 시 dopamine과 monoamine의 역할을 관찰하고자 하였다. 방 법 : 생후 5-6일 된 신생 흰쥐의 우측 총경동맥을 결찰 후 우측 선조체에 microdialysis probe를 삽입하였다. 2시간 동안의 안정기를 거친 후, probe를 통해 기저치를 수집하고, 바로 8% 산소에 2시간 동안 노출시키고, 2시간 동안 회복시키며 20분 간격으로 수집한 샘플을 HPLC를 통해 분석하였다. 결 과 : 1) Dopamine은 저산소-허혈기에 급격히 증가하는 경향을 보였으나 통계적 유의성은 없었다(P>0.05). 2) DOPAC은 저산소-허혈 동안 기저치의
Purpose : We intended to evaluate the effect of hypoxia-ischemia on extracellular striatal monoamine metabolism in neonatal rat brains by in vivo microdialysis. Methods : The right common carotid arteries of five or six-day old rats were surgically ligated, and the probes for microdialysis were inserted into the right striatum with stereotaxic instrument. After stabilization for two hours, artificial cerebrospinal fluid was infused via the probe for microdialysis and samples were collected during hypoxia-ischemia and recovery periods at 20 minute intervals. The concentrations of DA(dopamine), DOPAC(3,4-di-hydroxyphenyl acetic acid), HVA(homovanillic acid), NE(norepinephrine), and 5-HIAA(5-hydroxy indole-acetic acid) were measured by HPLC(high performance liquid chromatography) and the changes were analysed. Results : The striatal levels of dopamine metabolites such as DOPAC and HVA, were significantly decreased during hypoxia-ischemia, and increased to their basal level during reoxygenation(P<0.05). Dopamine mostly increased during hypoxia but statistically not significant(P>0.05). DOPAC showed the most remarkable decrease(