Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
권호 표지

Biocontrol of Rhizoctonia solani Damping-off of Cucumber by Bacillus cereus KJA-118

Bacillus cereus KJA-118을 이용한 오이 모잘록병의 생물학적 방제

  • An, Kyu-Nam (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University) ;
  • Jung, Woo-Jin (Department of Animal Science, Chonnam National University) ;
  • Chae, Dong-Hyun (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University) ;
  • Park, Ro-Dong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University) ;
  • Kim, Tae-Hwan (Department of Animal Science, Chonnam National University) ;
  • Kim, Yong-Woong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University) ;
  • Kim, Young-Cheol (Division of Applied Plant Science, APSRC, Chonnam National University) ;
  • Cha, Gyu-Suk (Division of Civil and Environmental Engineering, Kwangju University) ;
  • Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University)
  • 안규남 (전남대학교 생물환경학과) ;
  • 정우진 (전남대학교 동물자원학과) ;
  • 채동현 (전남대학교 생물환경학과) ;
  • 박노동 (전남대학교 생물환경학과) ;
  • 김태환 (전남대학교 동물자원학과) ;
  • 김용웅 (전남대학교 생물환경학과) ;
  • 김영철 (전남대학교 농생물학과) ;
  • 차규석 (광주대학교 환경공학과) ;
  • 김길용 (전남대학교 생물환경학과)
  • Received : 2003.03.25
  • Accepted : 2003.07.23
  • Published : 2003.08.30
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Abstract

A bacterium, KJA-118 showing a strong chitinase activity, was isolated and identified as Bacillus cereus. The strain produced maximum level of chitinase, when grown aerobically at $30^{\circ}C$ for 4 days in basal broth containing 1% colloidal chitin in the initial pH adjusted to 6.0. Among various carbon sources such as crab shell powder, chitin powder, colloidal chitin, and R. solani mycelium, maximum chitinase activity was found in culture broth supplemented with R. solani mycelium. When KJA-118 was incubated with R. solani, the cell wall of the fungus was found to be completely destroyed. SDS-PAGE and active staining results revealed that KJA-118 produced three isoforms of chitinase with molecular weights of 68 kDa, 47 kDa, and 37 kDa. When the suspension of KJA-118 was treated to cucumber seedlings, reducing rate of damping-off caused by R. solani was about 28.1%.

해안가 토양으로부터 강력한 chitinase 활성을 가진 Bacillus cereus KJA-118이 분리.동정되었다. B. cereus KJA-118은 1% colloidal chitin이 포함된 배지를 pH 6.0으로 조절한 후 $30^{\circ}C$ 에서 4일동안 호기적으로 배양했을 때 가장 높은 chitinase 효소활성을 보였다. 탄소원 (crab shell powder, chitin powder, colloidal chitin, and R. solani 균사)에 따른 chitinase 활성은 R. solani 균사를 사용했을 때 가장 높았다. Glycol chitin 0.01%가 포함된 gel에서 전기영동 후 활성 염색한 결과, B. cereus KJA-118에 의해 생산되는 chitinase는 분자량이 68, 47, 37 KDa인 3개의 isoform이 검출되었다. 액체 배지에서 미리 배양한 B. cereus KJA-118과 R. solani를 다시 혼합 배양했을 때, 곰팡이의 세포벽이 완전히 파괴되었다. R. solan가 감염된 토양에 B. cereus KJA-118의 배양액을 처리했을 때 28.1%의 오이 모잘록병 억제 효과를 확인하였다.

Keywords

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