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Biocontrol of Rhizoctonia solani Damping-off of Cucumber by Bacillus cereus KJA-118  

An, Kyu-Nam (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University)
Jung, Woo-Jin (Department of Animal Science, Chonnam National University)
Chae, Dong-Hyun (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University)
Park, Ro-Dong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University)
Kim, Tae-Hwan (Department of Animal Science, Chonnam National University)
Kim, Yong-Woong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University)
Kim, Young-Cheol (Division of Applied Plant Science, APSRC, Chonnam National University)
Cha, Gyu-Suk (Division of Civil and Environmental Engineering, Kwangju University)
Kim, Kil-Yong (Division of Applied Bioscience and Biotechnology, Institute of Agriculture Science and Technology, APSRC, Chonnam National University)
Publication Information
Korean Journal of Soil Science and Fertilizer / v.36, no.4, 2003 , pp. 247-255 More about this Journal
Abstract
A bacterium, KJA-118 showing a strong chitinase activity, was isolated and identified as Bacillus cereus. The strain produced maximum level of chitinase, when grown aerobically at $30^{\circ}C$ for 4 days in basal broth containing 1% colloidal chitin in the initial pH adjusted to 6.0. Among various carbon sources such as crab shell powder, chitin powder, colloidal chitin, and R. solani mycelium, maximum chitinase activity was found in culture broth supplemented with R. solani mycelium. When KJA-118 was incubated with R. solani, the cell wall of the fungus was found to be completely destroyed. SDS-PAGE and active staining results revealed that KJA-118 produced three isoforms of chitinase with molecular weights of 68 kDa, 47 kDa, and 37 kDa. When the suspension of KJA-118 was treated to cucumber seedlings, reducing rate of damping-off caused by R. solani was about 28.1%.
Keywords
Biological control; Chitinase activity; Bacillus cereus KJA-l18; Rhizoctonia solani;
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1 Asaka, 0., and M. Shoda. 1996. Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl. Environ. Microbiol. 62:4081-4085
2 Bradford, M.M. 1976. A rapid and sensitive method for the quantilation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254   DOI   PUBMED   ScienceOn
3 Downing, K.J., and J.A. Thomson. 2000. Introduction of the Serratia marcescens chiA gene into an endophytic Pseudomonas fluorescens for the biocontrol of phytopathogenic fungi. Can. J. Microbiol. 46:363-369   DOI   ScienceOn
4 Emmert, E.A.B., and J. Handelsman. 1999. Biocontrol of plant disease: a Gram positive perspective. FEMS Microbiol. Lett. 171:1-9   DOI   ScienceOn
5 Kang, S.W., M.J. Chung, I.H. Yeo, and D.H. Kim. 1998. Isolation and characterization of Pseudomonas vesicularis KW-15 for producing chitinase. Korean J. Chitm Chitosan. 3:303-312
6 Kwok, 0.C.H., P.C. Fahy, H.A.J. Hoitink, and G.A. Kuter. 1987. Interactions between bacteria and Trichoderma hamanum in suppression of Rhiwctonia damping-off in bark compost media. Phytopathol. 77:1206-1212   DOI
7 Osbum, R.M., J.L. Milner, E.S. Oplinger, R.S. Smith, and J. Handelsman. 1995. Eft'ect of Bucillus cereus UW85 on the yield of soybean at two field sites in Wisconsin. Plant Dis. 79:551-556   DOI   ScienceOn
8 Patil, R.S., V. Ghormade, and M.V. Deshpande. 2000. Chitinolytic enzymes: an exploration. Enzyme Microb. Tech. 26:473-483   DOI   ScienceOn
9 Shah, D.M. 1997. Genetic engineering for fungal and bacterial diseases. Curr. Opin. Biotech. 8:208-214   DOI   PUBMED   ScienceOn
10 Trudel, J., and A. Asselin. 1989. Detection of chitinase activity after polyacrylamide gel electrophoresis. Anal. Biochem. 178:362-366   DOI   ScienceOn
11 Young, J.P.W., H.L. Downer, and B.D. Eardly 1991. Phylogeny of phototrophic Rhiwbium strain BTAil by polymerase chain reaction-based sequencing of a 16S rRNAgene segment. J. Bacteriol. 173:2271-2277   DOI
12 Elad, Y., I. Chet, and Y. Henis. 1982. Degradation of plant pathogenic fungi by Trichoderma hurzicinum. Can. J. Microbiol. 28:719-725   DOI
13 Ordentlich, A., Y. Elad, and I. Chet. 1988. The role of chitinase of Serratia marcescens in biocontrol of Scterotium rolfsii. Phytopathol. 78:84-88
14 Singh, P.P., Y.C. Shin, C.S. Park, and Y.R. Chung. 1998. Biological control of Fusarium wilt of cucumber by chiyinolytic bacteria. Phytopathol. 89:92-99
15 Mabuchi, N., I. Hashizume, and Y. Araki. 2000. Characterization of chitinases excreted by Bacillus cereus CH. Can. J. Micorobiol. 46:370-375   DOI   ScienceOn
16 Pleban, S., L. Chernin, and I. Chet. 1997. Chitinolytic activity of an endophytic strain of Bacillus cereus. Lett. Appl. Microbiol. 25:284-288   DOI   ScienceOn
17 Godoy, G., R. Rodriguez-Kabana, and G. Morgan-Jones. 1982. Parasitism of eggs of Heterodera gtycines and Melodogyne arenaria by fungi isolated from cysts of H. glycines. Nematropica. 12:111-119
18 Wang, S.L., and J.R. Hwang. 2001. Microbial reclamation of shellfish wastes for the production of chitinases. Enzyme Microb. Technol. 28:376-382   DOI   ScienceOn
19 Inbar, J., and I. Chet. 1991. Evidence that chitinase produced by Aeroinonas caviae is involved in the biological control of soil-bome plant pathogens by this bacterium. Soil Biol. Biochem. 23:973-978   DOI   ScienceOn
20 National Institute of Agricultural Science and Technology. 2000. The method of soil and plant analysis. Rural Development Administration, Suwon, Korea
21 Bagnasco, P., L. Fuente, G. Gualtieii, F. Noya, and A. Arias. 1998. Fluorescent Pseudomonas spp. as biocontrol agents against forage legume root pathogenic fungi. Soil Biol. Biochem. 30:1317-1322   DOI   ScienceOn
22 Agrios, G. N. 1988. Plant Pathology (3rd ed.). Academic Press, INC, San Diego, USA
23 Trachuk, L.A., T.M. Shemiakina, G.G. Chestukhina, and V.M. Stepanov. 1996. Bacillus cereus chitinase: Isolation and characteristics. Biokhimiia. 61:357-368
24 Baek, J.M., C.R. Howell, and C.M. Kenerley. 1999. The role of an extracellular chitinase from Trichoderma virens Gv29-8 in the biocontrol of Rhizoctonia solani. Curr. Genet. 35:41-50   DOI   ScienceOn
25 Manocha, M.S. and V. Govindsamy. 1998. Chitinolytic enzymes of fungi and their involvement in biocontrol of plant pathogens. P. 309-327. In J.B. Greg and L.D Kuykendall (ed.) Plant-microbe interactions and biological control. Marcel Dekker, New York, USA