Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Effects of Puerariae radix herb-acupuncture on nitric oxide synthase expression in the hippocampus of alcohol-intoxicated Sprague-Dawley rats

갈근약침(葛根藥鍼)이 알콜 중독(中毒) 백서(白鼠) 해마(海馬)의 Nitric Oxide Synthase에 미치는 영향(影響)

  • Jung, Jae-houn (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Se-Myung University) ;
  • Kim, Ho-hyun (Department of Physiology, College of Oriental Medicine, Se-Myung University) ;
  • Lee, Eun-yong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Se-Myung University)
  • 정재훈 (세명대학교 한의과대학 침구학교실) ;
  • 김호현 (세명대학교 한의과대학 생리학교실) ;
  • 이은용 (세명대학교 한의과대학 침구학교실)
  • Received : 2003.05.17
  • Accepted : 2003.07.12
  • Published : 2003.08.20
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Abstract

Objective: The purpose of this study was to determine the effects of Puerariae radix(PR) herb-acupuncture on nitric oxide synthase(NOS) expression in hippocampus of alcohol-intoxicated Sprague-Dawley(SD) rats. Methods: SD rats were randomly assigned into 6 groups; normal group, control group, alcohol with herb-acupuncture group(0.3, 3, 30 and 300mg/kg PR). Normal groups were received with NaCl, while alcohol intoxication groups were injected intraperitoneally with alcohol(2g/kg) twice per day for 3 days. Herb-acupuncture groups were injected on Zhongwan(CV12) for 5 consecutive days. For the detection of NADPH-d-positive cells in hippocampus, immunohistochemistry was performed. Results: n control group, a significant decrease in NADPH-d-positive cells was observed compared to normal group. In alcohol with herb-acupuncture group, NADPH-d-positive cells increased significantly compared to control group. Conclusions: The present results revealed that NOS expression is enhanced in the hippocampus of SD rats through PR herb-acupuncture in an acute alcoholic intoxication condition.

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Acknowledgement

Supported by : 세명대학교